Visualization of brain perfusion by contrast-enhanced ultrasonography : a preliminary study in rabbit

为了评价超声造影剂（半乳糖帕米汀酸：Levovist）对兔脑血流灌注的显示作用，我们在麻醉状态下，在兔颅骨上开一个孔，用它作为声窗。采用右侧大脑中动脉夹闭法制作脑缺血模型。采用HDI 5000（ATL）型超声能量多普勒仪，探头频率5- 12 Mhz，分别在静脉注射造影剂（150 mg/人）前后获得能量多普勒图像和谐波图像。使用造影剂，小动脉清晰地显示了能量多普勒图像，但没有观察到脑实质的增强。在谐波图像上观察到脑灌注增强。增强在造影剂的第一次通过时最强。洗脱速度非常快，未观察到造影剂汇集。在缺血模型中，通过使用造影剂清楚地观察到缺血侧的脑灌注不足。结论：谐波超声造影可观察脑血流灌注情况，超声造影可用于评价脑缺血性疾病。

We evaluated the usefulness of contrast-enhance media of ultrasonography (galacutose parmitine acid : Levovist) to visualize the brain perfusion in rabbit.We made a hole in skull bone of the rabbit under anesthesia, and we used it for acoustic window. Ischemic model was made by occlusing the right middle cerebral artery. Power Doppler Images and Harmonic images before and after intravenous injection of contrast media (150mg/body) were obtained using HDI5000 (ATL) equipped with linear type 5-12Mhz probe. Using contrast media, small arteries were clearly visualized by Power Doppler images, but enhancement of brain parenchyma was not observed. Enhancement of brain perfusion was observed on Harmonic images. Enhancement was strongest on the first pass of the contrastmedia. Washout was very fast and pooling of contrast media was not observed. In ischemic models, hypoperfusion of the brain on the ischemic side was clearly observed by using contrast media. We concluded brain perfusion can be observed on Harmonic ultorasonographic imaging by using contrast media, and contrast enhanced ultrasonography may useful for evaluating ischemic diseases in human brain.