The effects of repetitive transcranial magnetic stimulation on neurogenesis

重复经颅磁刺激对神经发生的影响

• 批准号: 13671001
• 负责人: UKAI Satoshi
• 金额: $ 0.7万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671001/
• 关键词: TMS; neurogenesis; HFOs; SEPs; 神経細胞新; うつ病

The basis for therapeutic effect and optimum stimulus condition of rTMS (repetitive transcranial magnetic stimulation) is still unknown. We investigated the therapeutic mechanisms of rTMS in the light of neurogeneais in the dentate gyrus of the rats and the modulation of HFOs (high frequency oscillations) in the healthy human subjects.1. We aimed to examine the possibility that rTMS triggers hippocampal neurogenesis, and if so, to obtain optimum rTMS stimulus condition using neurogenesis as an indicator. Rats were given either real or sham rTMS. For the real rTMS group, rTMS was administered (10 Hz, 150 pulses, 120% motor threshold /day) for 14 days and the two groups were compared. The effects of rTMS .on neurogenesis in the dentate gyrus using bromodeoxyuridine (BrdU) immunohistochemistry, which identifies newly generated cells. Cells were labeled for neuronal nuclear protein (NeuN) to identify neurons.2. HFOs of SEPs (somatosensory evoked potentials) in 7 healthy human subjects were recorded before and after the slow rTMS on the primary sensory area (0.5 Hz, 50 pulses, 80% motor threshold). 6 of the 7 subjects showed significant increase of the amplitude and the power of HFOs after rTMS, while no subjects showed after sham rTMS. These results suggest that slow rTMS might directly increase the activity of GABAergic inhibitory interneurons in the cortex and suppress cortical excitability through these inhibitory interneurons. Studies of HFOs changes with other rTMS conditions are needed to obtain the optimum stimulus condition.

重复经颅磁刺激(RTMS)的疗效基础和最佳刺激条件尚不清楚。我们从大鼠齿状回神经发生和健康受试者高频振荡(HFO)的调节方面探讨了rTMS的治疗机制。我们的目的是研究rTMS触发海马神经发生的可能性，如果是这样的话，以神经发生为指标，获得最佳的rTMS刺激条件。给大鼠真的或假的rTMS。真正的rTMS组接受rTMS(10 Hz，150次脉冲，120%运动阈值/天)治疗14天，并与对照组进行比较。用溴脱氧尿嘧啶核苷(BrdU)免疫组织化学方法研究rTMS对齿状回神经发生的影响，以识别新生成的细胞。用神经元核蛋白(NeuN)标记细胞以鉴定神经元。记录7名健康受试者体感诱发电位(体感诱发电位)在0.5赫兹、50次脉冲、80%运动阈值慢速rTMS前、后的变化。7例受试者中有6例rTMS后HFO波幅和功率明显增加，而假rTMS后无1例出现。这些结果提示，慢rTMS可能通过直接增强大脑皮层GABA能抑制性中间神经元的活性，从而抑制皮质兴奋性。为了获得最佳的刺激条件，需要研究HFOS在其他rTMS条件下的变化。

项目成果

Shunsuke Kawaguchi: "Interictal spikes in the fusiform and inferior temporal gyri of an epileptic patient with colored elementary visual auras : a 5-year longitudinal MEG ECD study"Neuroreport. 14. 637-640 (2003)

Shunsuke Kawaguchi：“具有彩色基本视觉先兆的癫痫患者的梭状回和颞下回的发作间期尖峰：一项为期 5 年的纵向 MEG ECD 研究”Neuroreport。

K.Hirata, Y.Koga, K.Nagata, K.Yamazaki: "Recent Advances in Human Brain Mapping"Elsevier. 936 (2002)

K.Hirata、Y.Koga、K.Nagata、K.Yamazaki：“人类大脑图谱的最新进展”爱思唯尔。

Satoshi Ukai, Kazuhiro hinosaki, Ryouhei Ishii, Yuko-Mizuno atsumoto, Tsuyoahi Inouye, Norio Hirabuki, Toshiki Yoshimine, SE Robinson, Masatoshi Takeda In K. Miyoshi, CM Shapiro, M Gaviria, Y Morita (Eds.): "MEG functional neuroimaging of schizophrenic pa

Satoshi Ukai、Kazuhiro hinosaki、Ryouhei Ishii、Yuko-Mizuno atsumoto、Tsuyoahi Inouye、Norio Hirabuki、Toshiki Yoshimine、SE Robinson、Masatoshi Takeda In K. Miyoshi、CM Shapiro、M Gaviria、Y Morita（编辑）：“MEG 功能神经影像学

Satoshi Ukai, Kazuhiro hinosaki, Ryouhei Ishii, Asao Ogawa, Yuko Mizuno-Matsumoto, Tuyoshi Inouye, Norio Hirabuki, Toshiki Yoshimine, S.E. Robinson, Masatoshi Takeda, In K. Hirata, Y. Koga, K. Nagata, K. Yamazaki (Eds.): "Spatially Filtered MEG Analysis E

Satoshi Ukai、Kazuhiro hinosaki、Ryouhei Ishii、Asao Okawa、Yuko Mizuno-Matsumoto、Tuyoshi Inouye、Norio Hirabuki、Toshiki Yoshimine、S.E.

Yuko Mizuno-Matsumoto: "Landau-Kleffner syndrome : localization of epileptogenic lesion using wavelet-cross-correlation analysis"Epilepsy&Behavior. 2. 288-294 (2001)

Yuko Mizuno-Matsumoto：“Landau-Kleffner 综合征：使用小波互相关分析定位致癫痫病灶”癫痫