A Fundamental Study of Molecular and Developmental Pathology for Prevention and Treatment of Perinatal Hypoxic-Ischemic Brain Damage

围产期缺氧缺血性脑损伤防治的分子与发育病理学基础研究

• 批准号: 13671147
• 负责人: ITO Masayuki
• 金额: $ 2.18万
• 依托单位: National Institute of Neuroscience, National Center of Neurology and Psychiatry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671147/
• 关键词: Neonatal Hypoxic-Ischemic Brain Damage; Apoptosis

We studied an apoptosis phenomenon and its role on neonatal hypoxic-ischemic brain damage (HIE). From our results, we revealed apoptosis was an important role on forming perinatal HIE.The subjects were obtained by the below method. Two-weeks old mice were performed sham-operation and 5-minites ligation of common carotid arteries under ether anesthesia. At one to 14 days after operation, the research materials were removed.The brain tissue were stained with hematoxyline-eosin, nick end-labeling (TUNEL), immunohistochemistry (IHC) and in situ hybrydization (ISH).The results showed the number of apoptosis, which were detected by TUNEL, increased to 7 days after operation. And on the concerning of the protein and mRNA expression of c-fos and bcl-2 family, the peak was 3 days after operation.The furthermore studies are needed to clarify the pathomechanism of neonatal HIE.

本文研究了新生儿缺氧缺血性脑损伤（HIE）中细胞凋亡现象及其作用。结果表明，细胞凋亡在围生期HIE的形成中起重要作用。2周龄小鼠在乙醚麻醉下进行假手术和颈总动脉结扎5分钟。术后1 ~ 14 d取脑组织行苏木精-伊红染色、缺口末端标记（TUNEL）、免疫组化（IHC）和原位杂交（ISH）检测，结果显示：TUNEL检测到的凋亡细胞数在术后7 d逐渐增多。c-fos和bcl-2家族蛋白和mRNA表达均在术后3天达高峰，其发病机制有待进一步研究。

项目成果

Motonaga K, Itoh M, Becker LE, Goto Y, Takashima S: "levated expression of beta-site amyloid precursor protein cleaving enzyme 2 in brains of patients with Down syndrome"Neuroscience Letter. 326. 64-66 (2002)

Motonaga K、Itoh M、Becker LE、Goto Y、Takashima S：“唐氏综合症患者大脑中 β 位点淀粉样蛋白前体蛋白裂解酶 2 的表达升高”《神经科学快报》。

Motonaga K, Itoh M, Becker LE, Goto Y, Takashima S.: "levated expression of beta-site amyloid precursor protein cleaving enzyme 2 in brains of patients with Down syndrome"Neuroscience Letter. 326. 64-66 (2002)

Motonaga K、Itoh M、Becker LE、Goto Y、Takashima S.：“唐氏综合症患者大脑中 β 位点淀粉样蛋白前体蛋白裂解酶 2 的表达升高”《神经科学快报》。

Motonaga K, Itoh M, Hirayama A, Hirano S, Becker LE, Goto Y, Takashima S: "Up-regulation of E2F-1 in Down' s syndrome brain exhibiting neuropathological features of Alzheimer-type dementia"Brain Reserch. 905. 250-253 (2001)

Motonaga K、Itoh M、Hirayama A、Hirano S、Becker LE、Goto Y、Takashima S：“唐氏综合症大脑中 E2F-1 的上调，表现出阿尔茨海默型痴呆的神经病理学特征”大脑研究。

Hirayama A, Oka A, Itoh M, Tanaka F, Okoshi Y, Takashima S.: "Myelin transcription factor 1 (MyT1) immunoreactivity in infants with periventricular leukomalacia"Developmental Brain Research. 140. 85-92 (2003)

Hirayyama A、Oka A、Itoh M、Tanaka F、Okoshi Y、Takashima S.：“脑室周围白质软化症婴儿的髓磷脂转录因子 1 (MyT1) 免疫反应性”发育性脑研究。

Hirayama A, Oka A, Itoh M, Tanaka F, Okoshi Y, Takashima S: "Myelin transcription factor 1 (MyT1) immunoreactivity in infants with periventricular leukomalacia"Developmental Brain Research. 140. 85-92 (2003)

Hirayyama A、Oka A、Itoh M、Tanaka F、Okoshi Y、Takashima S：“脑室周围白质软化症婴儿的髓磷脂转录因子 1 (MyT1) 免疫反应性”发育性脑研究。