A Fundamental Study of Molecular and Developmental Pathology for Prevention and Treatment of Perinatal Hypoxic-Ischemic Brain Damage

我们研究了细胞凋亡现象及其在新生儿缺氧缺血性脑损伤(HIE)中的作用。根据我们的结果，我们发现细胞凋亡在围产期缺氧缺血性脑病的形成中起着重要的作用。两周龄小鼠在乙醚麻醉下行假手术，结扎颈总动脉5min。术后1~14d取出研究材料，对脑组织进行苏木精-伊红染色、缺口末端标记(TUNEL)、免疫组织化学(IHC)和原位杂交(ISH)，结果显示术后7d TUNEL检测到的细胞凋亡数增加。而c-fos和bcl2家族蛋白和mRNA的表达在术后3d达到高峰，其发病机制有待进一步研究。

We studied an apoptosis phenomenon and its role on neonatal hypoxic-ischemic brain damage (HIE). From our results, we revealed apoptosis was an important role on forming perinatal HIE.The subjects were obtained by the below method. Two-weeks old mice were performed sham-operation and 5-minites ligation of common carotid arteries under ether anesthesia. At one to 14 days after operation, the research materials were removed.The brain tissue were stained with hematoxyline-eosin, nick end-labeling (TUNEL), immunohistochemistry (IHC) and in situ hybrydization (ISH).The results showed the number of apoptosis, which were detected by TUNEL, increased to 7 days after operation. And on the concerning of the protein and mRNA expression of c-fos and bcl-2 family, the peak was 3 days after operation.The furthermore studies are needed to clarify the pathomechanism of neonatal HIE.