A Fundamental Study of Molecular and Developmental Pathology for Prevention and Treatment of Perinatal Hypoxic-Ischemic Brain Damage

我们研究了一种细胞凋亡现象及其在新生儿缺氧缺血性脑损伤（HIE）中的作用。结果表明，细胞凋亡在围产期HIE的形成中起重要作用。受试者采用以下方法获得。2周龄小鼠在乙醚麻醉下假手术结扎颈总动脉5分钟。术后1 ~ 14天取出研究材料。脑组织采用苏木精-伊红染色、缺口末端标记（TUNEL）、免疫组化（IHC）和原位杂交（ISH）染色。结果显示，术后第7天，TUNEL检测细胞凋亡数量明显增加。c-fos和bcl-2家族蛋白及mRNA表达在术后3 d达到高峰。新生儿HIE的发病机制有待进一步研究。

We studied an apoptosis phenomenon and its role on neonatal hypoxic-ischemic brain damage (HIE). From our results, we revealed apoptosis was an important role on forming perinatal HIE.The subjects were obtained by the below method. Two-weeks old mice were performed sham-operation and 5-minites ligation of common carotid arteries under ether anesthesia. At one to 14 days after operation, the research materials were removed.The brain tissue were stained with hematoxyline-eosin, nick end-labeling (TUNEL), immunohistochemistry (IHC) and in situ hybrydization (ISH).The results showed the number of apoptosis, which were detected by TUNEL, increased to 7 days after operation. And on the concerning of the protein and mRNA expression of c-fos and bcl-2 family, the peak was 3 days after operation.The furthermore studies are needed to clarify the pathomechanism of neonatal HIE.