Possible Role of Serotonin in Hepatic Ischemia and Reperfusion Injury in Rat

血清素在大鼠肝缺血和再灌注损伤中的可能作用

• 批准号: 13671247
• 负责人: SUZUKI Tatsuya
• 金额: $ 2.24万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671247/
• 关键词: serotonin; liver injury; ischemia reperfusion; 肝虚血再灌流障害

1. backgroundSrotoninn is known to induce platelet aggregation and to be a strong vasoconstrictor. Vascular stenosis by the formed thrombi and the vasocontraction aggravates ischemia of peripheral tissues and organs.2. aimThe aim of this study was to evaluate possible role of serotonin in hepatic ischemic reperfusion injury in rat by the selective S2-serotonergic receptor antagonist (5HTra).This study was conducted to investigate the infuence of serotonin on the hepatic microcirculation disturbance after hepatic ischemia and reperfusion (HIR) injury and to evaluate the effect of the selective S2-serotonergic receptor antagonist (5HTra) under these conditions.3. materials and methods8 week old Female S/D rats were used. Partial liver ischemia was performed by clamping the pedicles of the medial lobe for 30, 60, 90 minutes. In the 5HTra group, 5HTra was administered at a dose of 10mg/kg by intraabdominal injection 15min before ischemia and 15min before reperfusion.To determine the extent … More of hepatocellular damage during reperfusion, the levels of serum aspartate transaminase (U/L), serum alanine transaminase (U/L) and serum lactate dehydrogenase (U/L) were measured. Blood was withdrawn from the heart before ischemia, 30, 60, 90, 120 minutes. Change in the liver enzymes after ischemia reperfuson in the 5HTra group were compared with those in the control group.In addition, using specific antibody to the serotonin, to study 5-HT expression in the liver subjected to ischemia and reperfusion, immunochemical staining for 5-HT was then performed.4. resultThe intraabdominal administration of selective S2-serotonergic receptor antagonist a significant inhibition of the postreperfusion release of the liver enzymes.Serotonin was detected in sinusoid in the liver subjected to 90 min of ischemia followed by 60min of reperfusion.Thus ,we conclude that serotonin is strongly involved in the pathogenesis of ischemia reperfusion injury.5. conclusionThese and other results suggested that serotonin might play critical roles in the pathogenesis of hepatic ischemia and reperfusion injury. Less

1.背景技术已知Srotoninn诱导血小板聚集并且是强血管收缩剂。血栓形成和血管收缩引起的血管狭窄加重了周围组织和器官的缺血.本研究旨在探讨5-羟色胺（5-HT）在大鼠肝脏缺血再灌注损伤（HIR）中的作用，并观察5-HT受体拮抗剂（5 HTra）对肝脏缺血再灌注损伤后微循环障碍的影响。在这种情况下，3。材料与方法：8周龄雌性S/D大鼠。夹闭肝内叶蒂30、60、90分钟，造成部分肝缺血。5 HTra组于缺血前15 min和再灌注前15 min腹腔注射5 HTra 10 mg/kg， ...更多信息 检测血清谷丙转氨酶（U/L）、谷草转氨酶（U/L）和乳酸脱氢酶（U/L）水平。在缺血前、30、60、90、120分钟从心脏抽取血液。比较5 HTra组与对照组在缺血再灌注后肝酶的变化，并采用5-HT特异性抗体，对缺血再灌注后的肝脏进行5-HT免疫组化染色，研究5-HT在肝脏中的表达.结果腹腔注射选择性5-羟色胺受体拮抗剂可明显抑制再灌注后肝酶的释放，在缺血90 min再灌注60 min的肝血窦中可检测到5-羟色胺，提示5-羟色胺参与了缺血再灌注损伤的发病机制.结论5-羟色胺可能在肝缺血再灌注损伤的发病机制中起重要作用。少