A novel approach to analyze the immunological responses in human colorectal carcinoma

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1: Flowcytometric analyses of macrophages, dendritic cells, and lymphocytes isolated from colorectal cancer(CRC) tissue(1) We isolated mononuclear cells from colorectal tumors and normal colorectal tissues by enzymatic digestion and Ficoll-Paque separation.(2) Flowcytometric analyses showed more CD14-positive macrophages in the invasive margin of CRC than in normal mucosal fissue.(3) T cells derived from CRC were CD4-dominant compared to peripheral blood lymphocytes (PBL) and more than 90 % of those were memory T lymphocytes.(4) The T cells isolated from CRC were Th 1-dominant compared to PBL and positive for type l chemokines, CXCR3 and CCR5.(5) A mixed lymphocyte reaction experiment demonstrated that CD14-positive macrophages located in CRC possessed the ability to stimulate T cell responses.2: lmmunohistochemlcal analyses(1) lmmunohistochemical expression of the surface antigens in mononuclear cells in tumor was consistent with the data of the flowcytometric analyses.(2) Macrophages along the invasive margin of CRC were Fas ligand (FasL)-positive. The number of apoptotic cancer cells around the FasL-positive macrophages was correlated with the number of the FasL-positive macrophages.(3) CD4+ T cells and CD8+ T cells were positive for CXCR3 and CCR5. CD8+ T lymphocytes expressed RANITES, and cancer cells and macrophages expressed IP- 10.