A novel approach to analyze the immunological responses in human colorectal carcinoma

分析人类结直肠癌免疫反应的新方法

• 批准号: 13671275
• 负责人: MIZOI Takayuki
• 金额: $ 2.62万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671275/
• 关键词: colorectal carcinoma; tumorimmunology; macrophage; dendritic cell; lymphocyte; flowcytometry; 樹状細胞

1: Flowcytometric analyses of macrophages, dendritic cells, and lymphocytes isolated from colorectal cancer(CRC) tissue(1) We isolated mononuclear cells from colorectal tumors and normal colorectal tissues by enzymatic digestion and Ficoll-Paque separation.(2) Flowcytometric analyses showed more CD14-positive macrophages in the invasive margin of CRC than in normal mucosal fissue.(3) T cells derived from CRC were CD4-dominant compared to peripheral blood lymphocytes (PBL) and more than 90 % of those were memory T lymphocytes.(4) The T cells isolated from CRC were Th 1-dominant compared to PBL and positive for type l chemokines, CXCR3 and CCR5.(5) A mixed lymphocyte reaction experiment demonstrated that CD14-positive macrophages located in CRC possessed the ability to stimulate T cell responses.2: lmmunohistochemlcal analyses(1) lmmunohistochemical expression of the surface antigens in mononuclear cells in tumor was consistent with the data of the flowcytometric analyses.(2) Macrophages along the invasive margin of CRC were Fas ligand (FasL)-positive. The number of apoptotic cancer cells around the FasL-positive macrophages was correlated with the number of the FasL-positive macrophages.(3) CD4+ T cells and CD8+ T cells were positive for CXCR3 and CCR5. CD8+ T lymphocytes expressed RANITES, and cancer cells and macrophages expressed IP- 10.

一曰：从结直肠癌（CRC）组织中分离的巨噬细胞、树突状细胞和淋巴细胞的流式细胞仪分析（1）我们通过酶消化和Ficoll-Paque分离从结直肠肿瘤和正常结直肠组织中分离单核细胞。(2)流式细胞仪分析显示，大肠癌浸润边缘的CD 14阳性巨噬细胞比正常粘膜裂隙中的多。(3)与外周血淋巴细胞（PBL）相比，源自CRC的T细胞是CD 4-显性的，并且超过90%的那些是记忆T淋巴细胞。(4)与PBL相比，从CRC分离的T细胞是Th 1-显性的，并且对I型趋化因子CXCR 3和CCR 5呈阳性。(5)混合淋巴细胞反应实验表明，结直肠癌组织中CD 14阳性的巨噬细胞具有刺激T细胞反应的能力。2：免疫组化分析：（1）免疫组化结果显示，结直肠癌组织中单个核细胞表面抗原的表达与流式细胞术结果一致。(2)大肠癌浸润边缘沿着的巨噬细胞Fas配体（FasL）阳性。FasL阳性巨噬细胞周围的凋亡癌细胞数与FasL阳性巨噬细胞数相关。(3)CD 4 + T细胞和CD 8 + T细胞对CXCR 3和CCR 5呈阳性。CD 8 + T淋巴细胞表达RANITES，癌细胞和巨噬细胞表达IP- 10。

项目成果

Sugita J et al.: "Close association between Fas ligand-positive tumor-associated macrophages and apoptotic cancer cells along invasive margin of colorectal carcinoma : a proposal on tumor-host interactions"Jpn. J. Cancer Res.. 93. 320-328 (2002)

Sugita J等人：“Fas配体阳性肿瘤相关巨噬细胞与结直肠癌浸润边缘的凋亡癌细胞之间的紧密关联：关于肿瘤-宿主相互作用的建议”Jpn。

Sugita, J. et al.: "Close association between Fas 1 ligand(FasL ; CD95L)-positive tumor-associated macrophages and apoptotic cancer cells along invasive margin of colorectal carcinoma : A new paradigm of tumar-host interactions"Japanese Journal of Cancer

Sugita, J. 等人：“Fas 1 配体（FasL；CD95L）阳性肿瘤相关巨噬细胞与结直肠癌浸润边缘的凋亡癌细胞之间的紧密关联：肿瘤-宿主相互作用的新范例”日本癌症杂志