Monoclonal Antibody A7 Coupled to Magnetic Particles as a Contrast

单克隆抗体 A7 与磁性粒子偶联作为对比

• 批准号: 13671327
• 负责人: OTSUJI Eigo
• 金额: $ 2.3万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671327/
• 关键词: MONOCLONAL; ANTIBODY; A7; PANCREATIC CANCER; SUPERPARAMAGNETIC IRON OXIDE

We examined the retaining in vitro immunoreactivity of superparamagnetic iron oxide (SPIO) bound to Mab A7 and their in vivo distribution in nude mice bearing human pancreatic carcinoma xenografts were examined. A7-SPIO retained binding activities nearly identical to Mab A7. Significantly more of the radiolabeled A7-SPIO and ^<125>I labeled normal mouse IgG-SPIO disappeared from blood rapidly. As for all resected normal tissues, the accumulation levels of ^<125>I labeled A7-SPIO were lower than those for the tumors and decreased linearly with time. In MR imaging of the tumor bearing mude mice, signal intensity was reduced by the injection of A7-SPIO for the tumor

我们研究了保留在体外的免疫反应性的超顺磁性氧化铁（SPIO）结合单克隆抗体A7和他们在体内分布在荷人胰腺癌裸鼠异种移植物进行了检查。A7-SPIO保留了与Mab A7几乎相同的结合活性。显着更多的放射性标记的A7-SPIO和^<125>I标记的正常小鼠IgG-SPIO从血液中迅速消失。对于所有切除的正常组织，13 <125>I标记的A7-SPIO的积累水平低于肿瘤的积累水平，并随时间线性下降。在荷瘤小鼠的MR成像中，肿瘤注射A7-SPIO后信号强度降低，

项目成果

小林真一郎: "微粒子磁性鉄標識キメラ化モノクローナル抗体A7-Fab分画を用いた直腸癌診断のためのMRI造影剤の開発に関する研究"京都府立医大雑誌. 111. 29-37 (2002)

小林真一郎：“使用微粒磁性铁标记嵌合单克隆抗体 A7-Fab 级分开发用于直肠癌诊断的 MRI 造影剂的研究”京都府立医科大学学报 111. 29-37 (2002)。

小林真一郎: "微粒子磁性鉄標識キメラ化モノクローナル抗体A7Fab分画を用いた直腸癌診断のためのMRI造影剤の開発に関する研究"京都府立医大雑誌. 111. 29-37 (2002)

小林真一郎：“使用微粒磁性铁标记的嵌合单克隆抗体A7Fab级分开发用于直肠癌诊断的MRI造影剂的研究”京都府立医科大学学报111. 29-37 (2002)。

Matsumura H, Otsuji E他: "Potential usefulness of 99mTc-labeled monoclonal antibody A7 for immunoscintigraphy of human pancreatic carcinoma"Anticancer Research. 21. 3433-3438 (2001)

Matsumura H、Otsuji E 等人：“99mTc 标记的单克隆抗体 A7 对于人类胰腺癌免疫闪烁扫描的潜在用途”Anticancer Research 21. 3433-3438 (2001)。

Matsumura H, Otsuji E.他: "Potential usefulness of 99mTc-labeled monoclonal antibody A7 for immunoscintigraphy of human pancreatic carcinoma"Anticancer Research. 21. 3433-3438 (2001)

Matsumura H、Otsuji E.等人：“99mTc 标记的单克隆抗体 A7 对于人类胰腺癌免疫闪烁扫描的潜在用途”Anticancer Research 21. 3433-3438 (2001)。

Hiroomi Matsumura, et al.: "Potential usefulness of 99mTc-labeled monoclonal antibody A7 for immunoscintigraphy of human pancreatic carcinoma"Anticancer Research. 21. 3433-3438 (2002)

Hiroomi Matsumura 等人：“99mTc 标记的单克隆抗体 A7 对于人类胰腺癌免疫闪烁扫描的潜在用途”抗癌研究。