Immunoguided Surgery Using Monoclonal Antibody A7 Coupled to Magnetic Particles

我们检测了与单抗A7结合的铁磁性颗粒在体外的保留免疫反应性，并检测了它们在荷人胰腺癌裸鼠体内的分布。A7-LIGNOSITE FML保留了与单抗A7几乎相同的结合活性。在肿瘤中累积的放射性标记的A7-LIGNOSITE FML显著多于正常小鼠的Ig G-LIGNOSITE FML(P&lt；0.05)。A7-LIGNOSITE FML和^&lt；125&gt；I标记的正常小鼠Ig G-LIGNOSITE FML迅速从血液中消失。在所有切除的正常组织中，~(125)&gt；I标记的A7-LIGNOSITE FML的积聚水平均低于肿瘤组织，且随时间呈线性下降。

We examined the retaining in vitro immunoreactivity of ferromagnetic particles bound to Mab A7 and their in vivo distribution in nude mice bearing human pancreatic carcinoma xenografts were examined. A7-Lignosite FML retained binding activities nearly identical to Mab A7. Significantly more of the radiolabeled A7-Lignosite FML accumulated in the tumor than did normal mouse IgG-Lignosite FML (P<0.05). A7-Lignosite FML and ^<125>I labeled normal mouse IgG-Lignosite FML disappeared from blood rapidly. As for all resected normal tissues, the accumulation levels of ^<125>I labeled A7-Lignosite FML were lower than those for the tumors and decreased linearly with time.