Immunoguided Surgery Using Monoclonal Antibody A7 Coupled to Magnetic Particles

使用与磁性粒子偶联的单克隆抗体 A7 进行免疫引导手术

• 批准号: 09671329
• 负责人: OTSUJI Eigo
• 金额: $ 1.98万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671329/
• 关键词: MONOCLONAL; ANTIBODY; A7; PANCREATIC CANCER; 手術; リンパ節転移

We examined the retaining in vitro immunoreactivity of ferromagnetic particles bound to Mab A7 and their in vivo distribution in nude mice bearing human pancreatic carcinoma xenografts were examined. A7-Lignosite FML retained binding activities nearly identical to Mab A7. Significantly more of the radiolabeled A7-Lignosite FML accumulated in the tumor than did normal mouse IgG-Lignosite FML (P<0.05). A7-Lignosite FML and ^<125>I labeled normal mouse IgG-Lignosite FML disappeared from blood rapidly. As for all resected normal tissues, the accumulation levels of ^<125>I labeled A7-Lignosite FML were lower than those for the tumors and decreased linearly with time.

我们检查了保留在体外的免疫反应性的铁磁性颗粒结合到单抗A7和它们在体内分布在裸鼠人胰腺癌异种移植物进行了检查。A7-亮氨酸位点FML保留与Mab A7几乎相同的结合活性。放射性标记的A7-Lactobacillus位点FML在肿瘤中的蓄积量显著高于正常小鼠IgG-Lactobacillus位点FML（P<0.05）。A7-Lactobacillus site FML和~<125>（60）I标记的正常小鼠IgG-Lactobacillus site FML从血液中迅速消失。对于所有切除的正常组织，<125>标记的A7-Lactobacillus位点FML的积累水平低于肿瘤的积累水平，并随时间线性下降。

项目成果

Eigo Otsuji: "Recent advances in Surgical Treatment Have Improved the Survival of Patients with Gastric Cancer" Cancer. 82. 1233-1237 (1998)

Eigo Otsuji：“手术治疗的最新进展提高了胃癌患者的生存率”癌症。

Eigo Otsuji, et.al.: "Recent advances in surgical treatment have improved the survival of patients with gastric carcinoma" Cancer. 82. 1233-1237 (1998)

Eigo Otsuji 等人：“手术治疗的最新进展提高了胃癌患者的生存率”。

Hiroshi Tsuruta: "Application of tumor specific monoclonal antibody A7-magnetic iron oxide particle conjugate for immunoguided surgery" Journal of Kyoto Prefectural University of Medicine. 106. 1249-1257 (1997)

Hiroshi Tsuruta：“肿瘤特异性单克隆抗体 A7-磁性氧化铁颗粒缀合物在免疫引导手术中的应用”京都府立医科大学学报。

大辻英吾 他: "キメラ化A7-Fab分画結合Neocarzinostatinの膵癌ミサイル療法への有用性" 癌と化学療法. 24(15). 2285-2287 (1997)

Eigo Otsuji 等人：“嵌合 A7-Fab 片段结合的新制癌菌素用于胰腺癌导弹治疗”，Cancer and Chemotherapy 24(15) (1997)。

山本 一仁: "モノクローナル抗体A7のradio immunoguided surgery(RIGS)への応用" Biotherapy. 13. 102-104 (1999)

Kazuhito Yamamoto：“单克隆抗体 A7 在放射免疫引导手术 (RIGS) 中的应用”生物治疗。13. 102-104 (1999)