Monoclonal Antibody Conjugated to Gadolinium as a Contrast Agent for Magnetic Resonance Imaging of Human Rectal Carcinoma

与钆缀合的单克隆抗体作为人类直肠癌磁共振成像的造影剂

• 批准号: 15591428
• 负责人: OTSUJI Eigo
• 金额: $ 2.24万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591428/
• 关键词: GADRINIUM; MNOCLONAL ANTIBODY; MR imaging; contrast agent; 膵癌

Local disease is the most frequent recurrence pattern of rectal carcinoma, and its prognosis is not good. To detect local recurrence as early as possible, we produced the monoclonal antibody A7-gadolinium(Mab A7-Gd) conjugate as a contrast agent for magnetic resonance imaging to distinguish between carcinoma and normal tissue. We examined the in vitro immunoreactivity of Mab A7 coupled to Gd by chelate, isothiocyanobenzyl EDTA and its in vivo distribution in nude mice with human colorectal carcinoma. Mab A7-Gd retained binding activities that were nearly identical to intact Mab A7. More radiolabelled Mab A7-Gd accumulated in the tumour than normal mouse IgG-Gd. Both Mab A7-Gd and normal mouse IgG-Gd disappeared from blood linearly over time. Accumulation levels in normal tissues decreased linearly over time but were lower than those in tumours. Mab A7 conjugated to gadolinium is potentially suitable as a contrast agent for MR imaging of local rectal carcinoma recurrence.

局部病变是直肠癌最常见的复发方式，预后不佳。为了尽早检测局部复发，我们生产了单克隆抗体A7-钆（Mab A7-Gd）结合物作为磁共振成像的对比剂，以区分癌和正常组织。我们研究了单抗A7偶联Gd螯合物，异硫氰酸苄EDTA的体外免疫反应性和其在裸鼠体内分布与人大肠癌。Mab A7-Gd保留与完整Mab A7几乎相同的结合活性。放射性标记的Mab A7-Gd在肿瘤中比正常小鼠IgG-Gd积累更多。Mab A7-Gd和正常小鼠IgG-Gd均随时间线性地从血液中消失。正常组织中的蓄积水平随时间呈线性下降，但低于肿瘤中的蓄积水平。单克隆抗体A7结合钆是潜在的适合作为一种对比剂的MR成像局部直肠癌复发。

项目成果

E.Otsuji, et al.: "Application of 99m Tc labeled chimeric Fab framents of monoclonal antibody A7 for immunoscintigraphy of pancreatic carcinoma"Journal of Surgicul Oncology. 84(3). 160-165 (2003)

E.Otsuji等人：“单克隆抗体A7的99m Tc标记的嵌合Fab框架在胰腺癌免疫闪烁显像中的应用”《肿瘤外科杂志》。

E.Otsuji, et al.: "Effects of idiotypic human anti-mouse antibody against in vitro binding and antitumor activity of a monoclonal antibody-drug conjugate"Hepatogastroenterology. 50. 380-384 (2003)

E.Otsuji等人：“独特型人抗小鼠抗体对单克隆抗体-药物缀合物的体外结合和抗肿瘤活性的影响”肝胃肠病学。

Decreased production of anti-mouse antibody after administration of human/mouse chimeric monoclonal antibody-neocarzinostatin conjugate to human

给予人人/小鼠嵌合单克隆抗体-新制癌菌素缀合物后抗小鼠抗体的产生减少

• 发表时间: 2003
• 期刊: Hepatogastroenterology 50
• 作者: Yata Y;Otsuji E;他
• 通讯作者: 他

Decreased production of anti-mouse antibody after administration of human/mouse chimeric monoclonal antibody-neocarzinostatin conjugate to human.

给予人人/小鼠嵌合单克隆抗体-新制癌菌素缀合物后，抗小鼠抗体的产生减少。

• 发表时间: 2003
• 期刊: Hepatogastroenterology. 50(49)
• 作者: Yata Y;Otsuji E;Okamoto K;Tsuruta H;Kobayashi S Toma A;Yamagishi H.
• 通讯作者: Yamagishi H.

Application of 99mTc labeled chimeric Fab fragments of monoclonal antibody A7 for immunoscintigraphy of pancreatic carcinoma

• DOI: 10.1002/jso.10311
• 发表时间: 2003-11-01
• 期刊: JOURNAL OF SURGICAL ONCOLOGY
• 影响因子: 2.5
• 作者: Otsuji, E;Matsumura, H;Yamagishi, H
• 通讯作者: Yamagishi, H