Angiogenic effect of eNOS gene-contained bone marrow cell transplantation

含eNOS基因的骨髓细胞移植的血管生成作用

• 批准号: 13671413
• 负责人: HORIMOTO Hitoshi
• 金额: $ 1.15万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671413/
• 关键词: angiogenesis; bone marrow cell; nitric oxide; ischemic tolerance; 虚血心; HGF; NO(一酸化窒素)

Angiogenic effect of intra-arterial bone marrow cell transplantation into rat hindlimb ischemia1) Rat hindlimb ischemia model was produced by removing unilateral femoral artery.2) Bone marrow mononuclear cells of another rat were implanted into muscles in ischemic area.3) Bone marrow mononuclear cells of another rat were implanted into artery close to ischemic area.4) Intra-arterial bone marrow transplantation was shown to induce angiogenic effects similar to intra-muscular injection evident by angiography, thermogram and immunohistochemical analysis.Angiogenic effect of intra-coronary bone marrow cell transplantation into rat ischemic myocardium1) Rat ischemic myocardium was produced by ligating the left anterior descending coronary artery (LAD).2) Two weeks after the LAD ligation, heart was excited and bone marrow mononuclear cells were implanted into coronary artery via aortic root. Then, the heart was transplanted into another rat abdomen by heterotopic heart transplantation method … More .3) Two weeks after the heterotopic heart transplantation, heart was explanted and was subjected to Langendorff perfusion system to determine ischemic response against ischemia reperfusion insult and angiogenic effects.4) There were no significant differences between bone marrow transplantation group and control group in terms of ischemic response, whereas significant angiogenic effects were observed in bone marrow transplantation group.Effects of eNOS gene delivery on angiogenesis and ischemic response1) Rat ischemic myocardium was produced by ligating the left anterior descending coronary artery (LAD).2) Two weeks after the LAD ligation, heart was excised and bone marrow mononuclear cells and eNOS cDNA were implanted into coronary artery via aortic root. Then, the heart was transplanted into another rat abdomen by heterotopic heart transplantation method.3) Two weeks after the heterotopic heart transplantation, hear was explanted and was subjected to Langendorff perfusion system to determine ischemic response against ischemia reperfusion insult and angiogenic effects.4) The eNOS gene delivery significantly increased coronary flow compared to bone marrow cell transplantation alone and expression of NOS was determined in neovascular formation induced by bone marrow cell transplantation. Less

骨髓细胞动脉内移植对大鼠后肢缺血的促血管生成作用1）通过切除一侧股动脉建立大鼠后肢缺血模型。2）将另一只大鼠的骨髓单个核细胞移植到缺血区的肌肉中。3）将另一只大鼠的骨髓单个核细胞移植到缺血区附近的动脉中。4）将骨髓单个核细胞动脉内移植到缺血区附近的肌肉中。动脉骨髓移植显示出诱导类似于血管造影术证实的肌内注射的血管生成作用，骨髓细胞冠状动脉内移植对大鼠缺血心肌血管生成的影响结扎大鼠冠状动脉左前降支（LAD）造成缺血心肌。2）LAD结扎后2周，心脏兴奋，经主动脉根部将骨髓单个核细胞植入冠状动脉。然后采用异位心脏移植法将心脏移植到另一只大鼠腹部 ...更多信息 （3）移植后2周，取心脏，用Langendorff灌流系统检测缺血再灌注损伤的反应及血管生成情况。（4）骨髓移植组与对照组相比，缺血反应无显著性差异，eNOS基因转染对血管生成和缺血反应的影响1）结扎大鼠冠状动脉左前降支（LAD）造成缺血心肌。2）LAD结扎后2周，切取心脏，经主动脉根部将骨髓单个核细胞和eNOS cDNA植入冠状动脉内。3）异位心脏移植后2周，取心脏，取左心室，取右心室将听力恢复并进行Langendorff灌注系统以确定针对缺血再灌注损伤的缺血反应和血管生成效应。4）与单独骨髓细胞移植相比，eNOS基因递送显著增加了冠状动脉流量，骨髓细胞移植诱导的新生血管形成。少