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Study of effect of a carbohydrate chain gene on the characteristics of ovarian cancer cells

糖链基因对卵巢癌细胞特性影响的研究

基本信息

批准号：
13671755
负责人：
KIGUCHI Kazushige
金额：
$ 2.24万
依托单位：
St. Marianna University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671755/
关键词：
Ovarian cancer carbohydrate chain gene fucosyltransferase type I carbohydrate chain

项目摘要

In this study, we investigated how a carbohydrate chain gene affects the cell characteristics of ovarian cancer and its prognosis. That is, how the properties of the cancer cells were affected by the change In carbohydrate chains of the cells induced by introduction of the gene of fucosyltransferase that is an enzyme modifying non-reducing end of carbohydrate chains of cell membrane was examined. The role of sugar chains at that time was also analyzed. It is known that H gene is participating not only in exhibiting ABO blood types but also in functioning such as cell adhesion. Hence, clarification of biochemical changes in carbohydrate chains of cell membrane induced by introduction of H gene that is engaged in synthesis of carbohydrate chains was attempted, and the following results were obtained.Results1. Cells to which human H gene was introduced were smaller and showed denser cell-to-cell adhesion compared with the control cells.2. Cellular sialic acid content in the gene-introduced cells was low, indicating antagonistic activity between fucosylation and *lation.3. Since lacto-series type I carbohydrate chains were detected in only the gene-introduced cells, fucosylation seemed to be induced by the introduced gene.4. Susceptibility to 5-Fu was examined. Owing to introduction of fucosyltransferase, the membrane permeability to hydrophobic molecules rose, and the susceptibility of the cells to anticancer drugs increased.These results suggested that modification of surface carbohydrate chains of ovarian cancer cells may be applied to the treatment of ovarian cancer for improvement of the prognosis.

在这项研究中，我们研究了碳水化合物链基因如何影响卵巢癌的细胞特性及其预后。也就是说，检测了通过导入岩藻糖基转移酶的基因诱导的细胞碳水化合物链的变化如何影响癌细胞的性质，岩藻糖基转移酶是修饰细胞膜碳水化合物链的非还原性末端的酶。还分析了当时糖链的作用。已知H基因不仅参与ABO血型的表现，而且还参与细胞粘附等功能。因此，本研究试图阐明通过导入参与糖链合成的H基因而引起的细胞膜糖链的生化变化，并获得了以下结果。与对照组相比，导入人H基因的细胞体积更小，细胞间粘附更密.导入基因的细胞唾液酸含量较低，表明岩藻糖基化和 * 化之间存在拮抗作用.由于仅在基因导入的细胞中检测到乳糖系列I型碳水化合物链，因此岩藻糖基化似乎由导入的基因诱导。对5-Fu敏感性进行了检测。由于岩藻糖基转移酶的引入，细胞膜对疏水分子的通透性增加，细胞对抗癌药物的敏感性增加，提示卵巢癌细胞表面糖链的修饰可能用于卵巢癌的治疗，以改善预后。