Analysis of the molecular bases and the expression of the Lewis^x sugar chain, which may account for the high dissemination potential.
Analysis of the molecular bases and the expression of the Lewis^x sugar chain, which may account for the high dissemination potential.
批准号:
09671721
负责人:
KIGUCHI Kazushige
金额:
$1.92万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998
中文摘要
在粘液性囊腺癌、浆液性囊腺癌和透明细胞癌等多种卵巢肿瘤中,粘液性囊腺癌表现出独特的糖鞘糖脂组成。粘液性囊腺癌中90%以上的酸性鞘糖脂由硫代磷脂组成,在正常卵巢中几乎检测不到,在卵巢肿瘤类型中含量低于40%。将来源于卵巢透明细胞腺癌的人肿瘤细胞系RMG-1细胞注射到裸鼠体内,发现从肠系膜肿瘤结节中获得的细胞比原始RMG-1细胞形成更多、更大的肿瘤结节。Rmg-1-h细胞从肿瘤结节中转移到培养物中,在体内传至第4代后,显示出与直接从组织中扩散的细胞一样高的扩散潜能,并且远远高于rmg…。多-1个细胞。为了评估RMG-1和RMG-1-h细胞不同生物学特性的分子基础,我们比较了两种细胞中各种糖类抗原的含量和表达。与流式细胞术检测RMG-1细胞中Le^x阳性细胞的宽直方图不同,RMG-1-h细胞的直方图中去除了抗Le^x抗体弱阳性和中等阳性的细胞,导致强表达Le^x的细胞增多,这可能是RMG-1细胞具有较高的播散潜能的原因。此外,抗Le^x抗体可显著抑制RMG-1细胞与间皮细胞的黏附,提示Le^x介导了卵巢癌细胞与间皮细胞之间的细胞间相互作用。因此,卵巢癌细胞的高扩散潜能是由Le^x决定簇介导的,携带Le^x的细胞通过重复体内传代进入裸鼠体内而得到丰富。较少
英文摘要
Among several human ovarian tumors, which include mucinous cystadenocarcinoma, serous cystadenocarcinoma, and clear cell adenocarcinoma, the mucinous cystadenocarcinoma showed a unique glycosphingolipid composition. More than 90% of the acidic glycosphingolipids in the mucinous cystadenocarcinoma is comprised of sulfolipids, which are hardly detected in normal ovary and are contained in concentrations of less than 40% in the type of ovarian tumors.Cells of the human tumor cell line RMG- 1, derived from a clear cell adenocarcinoma of the ovary were injected intraperitoneally into nude mice, and the cells obtained from the tumor nodules in the mesenterium were found to form a larger number of, and larger-sized, tumor nodules than the original RMG- 1 cells. The RMG- 1-h cells, transferred into culture from the tumor nodules after a 4th in vivo passage, showed a dissemination potential as high as that of cells disseminating directly from the tissues, and exceedingly higher than that of RMG … More - 1 cells. To assess the molecular bases of the different biological properties of RMG- 1 and RMG- 1 -h cells, we compared the content and expression of various carbohydrate antigens in both cells. In contrast to the broad histogram for the Le^x-bearing cells among RMG- 1 cells in flow cytometry, the weakly and moderately positive cells toward anti-Le^x antibody were found to be eliminated from the histogram for the RMG- 1 -h cells, resulting in the enrichment of cells strongly expressing Le^x, which may account for the high dissemination potential. In addition, the adhesion of RMG- 1 cells to mesothelial cells was found to be significantly inhibited by pretreatment of the cells with anti-Le^x antibody, indicating Le^x mediated cell-to-cell interaction between ovarian cancer cells and mesothelial cells. Thus, the high dissemination potential of ovarian cancer cells was shown to be mediated by the Le^x-determinant and the Le^x-bearing cells are enriched by repeated in vivo passage of the cells into nude mice. Less
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Kazushige Kiguchi: "A patient-like orthotopic implantation nude mouse model of highly metastatic human ovarian cancer." Clinical and Experimental Metastasis. 16 (in print). (1999)
Kazushige Kiguchi:“高度转移性人类卵巢癌的患者样原位植入裸鼠模型。”
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Kazushige Kiguchi: "Coexpression of Cholesterol sulfate and cytokeratin in the well differentiated type of squamous tumors of human uterine cervix." Clinical Cancer Research. 4. 2985-2990 (1998)
Kazushige Kiguchi:“硫酸胆固醇和细胞角蛋白在人宫颈分化良好的鳞状肿瘤中的共表达。”
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Kazushige Kiguchi: "Selection of human ovarian carcinoma cells with high dissemination potential by repeated passage of cells in vivo into nude mice, and involvement of Le^x-determinant in the dissemination potential." Japanese Journal of Cancer Research.
Kazushige Kiguchi:“通过将细胞在体内重复传代到裸鼠体内,选择具有高传播潜力的人卵巢癌细胞,并且 Le^x 决定子参与传播潜力。”
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Kazushige Kiguchi: "Establishment and characterization of melanoma cell line derived from malignant melanoma of human uterine cervix." Human Cell. 11(2). 93-100 (1998)
Kazushige Kiguchi:“源自人类子宫颈恶性黑色素瘤的黑色素瘤细胞系的建立和表征。”
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Kazushige Kiguchi: "Selection of human ovarian carcinoma cells with high dissemination potential by repeated passage of cells in vivo into nude mice,and involvement of Le^x-determinant in the dissemination potential." Japanese Journal of Cancer Research.
Kazushige Kiguchi:“通过将细胞在体内重复传代到裸鼠体内,选择具有高传播潜力的人卵巢癌细胞,并且 Le^x 决定子参与传播潜力。”
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Optimization of chemotherapy of ovarian cancer as an indicator of transporter expression and glycolipid
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批准号:20591965
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.83万
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财政年份:2008
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负责人:KIGUCHI Kazushige
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依托单位:
A trial at developing molecular-targeted therapy of ovarian cancer using a molecular chaperon heat shock protein(Hsp) as the target
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批准号:18591849
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.48万
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财政年份:2006
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负责人:KIGUCHI Kazushige
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依托单位:
Study of effect of a carbohydrate chain gene on the characteristics of ovarian cancer cells
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批准号:13671755
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.24万
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财政年份:2001
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负责人:KIGUCHI Kazushige
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依托单位:
Analysis and Clinical Significance on the Carbohydrate Chain Gene related to the Peritoneal Dissemination of Ovarian Cancer.
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批准号:11671659
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.43万
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财政年份:1999
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负责人:KIGUCHI Kazushige
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依托单位:
Glycosphingolipids of various human ovarian tumors
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批准号:03807109
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1991
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负责人:KIGUCHI Kazushige
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依托单位:
海外基金