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Analysis of the molecular bases and the expression of the Lewis^x sugar chain, which may account for the high dissemination potential.

对 Lewis^x 糖链的分子碱基和表达进行分析，这可能是其高传播潜力的原因。

基本信息

批准号：
09671721
负责人：
KIGUCHI Kazushige
金额：
$ 1.92万
依托单位：
St.Marianna Univ.School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Among several human ovarian tumors, which include mucinous cystadenocarcinoma, serous cystadenocarcinoma, and clear cell adenocarcinoma, the mucinous cystadenocarcinoma showed a unique glycosphingolipid composition. More than 90% of the acidic glycosphingolipids in the mucinous cystadenocarcinoma is comprised of sulfolipids, which are hardly detected in normal ovary and are contained in concentrations of less than 40% in the type of ovarian tumors.Cells of the human tumor cell line RMG- 1, derived from a clear cell adenocarcinoma of the ovary were injected intraperitoneally into nude mice, and the cells obtained from the tumor nodules in the mesenterium were found to form a larger number of, and larger-sized, tumor nodules than the original RMG- 1 cells. The RMG- 1-h cells, transferred into culture from the tumor nodules after a 4th in vivo passage, showed a dissemination potential as high as that of cells disseminating directly from the tissues, and exceedingly higher than that of RMG … More - 1 cells. To assess the molecular bases of the different biological properties of RMG- 1 and RMG- 1 -h cells, we compared the content and expression of various carbohydrate antigens in both cells. In contrast to the broad histogram for the Le^x-bearing cells among RMG- 1 cells in flow cytometry, the weakly and moderately positive cells toward anti-Le^x antibody were found to be eliminated from the histogram for the RMG- 1 -h cells, resulting in the enrichment of cells strongly expressing Le^x, which may account for the high dissemination potential. In addition, the adhesion of RMG- 1 cells to mesothelial cells was found to be significantly inhibited by pretreatment of the cells with anti-Le^x antibody, indicating Le^x mediated cell-to-cell interaction between ovarian cancer cells and mesothelial cells. Thus, the high dissemination potential of ovarian cancer cells was shown to be mediated by the Le^x-determinant and the Le^x-bearing cells are enriched by repeated in vivo passage of the cells into nude mice. Less

在包括粘液性囊腺癌、浆液性囊腺癌和透明细胞腺癌的几种人类卵巢肿瘤中，粘液性囊腺癌显示出独特的鞘糖脂组成。粘液性囊腺癌中超过90%的酸性鞘糖脂由硫脂组成，硫脂在正常卵巢中几乎检测不到，在卵巢肿瘤类型中浓度低于40%。将来源于卵巢透明细胞腺癌的人肿瘤细胞系RMG- 1的细胞腹膜内注射到裸鼠中，并且发现从肠系膜中的肿瘤结节获得的细胞形成比原始RMG- 1细胞更多数量和更大尺寸的肿瘤结节。在体内第4代后从肿瘤结节转移到培养物中的RMG- 1-h细胞显示出与直接从组织中播散的细胞一样高的播散潜力，并且远远高于RMG的播散潜力 ...更多信息 - 1细胞。为了评估RMG- 1和RMG- 1 -h细胞不同生物学特性的分子基础，我们比较了两种细胞中各种糖抗原的含量和表达。与流式细胞术中RMG- 1细胞中携带Le^x的细胞的宽直方图相反，发现抗Le ^x抗体的弱阳性和中度阳性细胞从RMG- 1 -h细胞的直方图中消除，导致强表达Le^x的细胞富集，这可能是高传播潜力的原因。此外，发现用抗Le ^x抗体预处理细胞显著抑制RMG- 1细胞与间皮细胞的粘附，表明Le^x介导卵巢癌细胞与间皮细胞之间的细胞间相互作用。因此，卵巢癌细胞的高扩散潜力被证明是由Le^x决定簇介导的，并且通过将细胞重复体内传代到裸鼠中来富集携带Le^x的细胞。少