Optimization of chemotherapy of ovarian cancer as an indicator of transporter expression and glycolipid

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To further characterize the molecular basis on anticancer drug-resistance of human ovarian carcinoma, we compared serous ovarian carcinoma derived 2008 cells with the taxol-resistant Px2 and cisplatin-resistant C13 cells. Although the amounts of major lipids, i.e. cholesterol, ceramides and phospholipids, in the resistant cells were same with those in the sensitive cells, Gb3Cer was characteristically increased in the resistant cells, irrespective of whether the resistance was to taxol or cisplatin. Also, gangliosides GM1 and GD1a were increased in the taxol-resistant cells, but were absent in the cisplatin-resistant cells. Expression of MDR1 gene was significantly enhanced in the taxol-resistant cells. Thus, coexpression of transporter proteins and glycolipids was suggested to enhance the activity of transporter proteins by strengthening the molecular packing through the hydrogen bonds between glycolipids and proteins in the raft structures of biomembrane.