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Glycosphingolipids of various human ovarian tumors

各种人卵巢肿瘤的鞘糖脂

基本信息

批准号：
03807109
负责人：
KIGUCHI Kazushige
金额：
$ 1.22万
依托单位：
Keio University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

1. Glycosphingolipids (GSLs) of Various Human Ovarian Tumors:Among the human ovarian tumors, mucinous cystadenocarcinoma, serous cystadenocarcinoma, and clear cell adenocarcinoma, the mucinous cystadenocarcinoma showed a unique GSL composition. More than 90% of the acidic GSL in the mucinous cystadenocarcinoma was comprised of sulfolipids, rarely detected in normal ovary and found in concentrations of less than 40% in the other types of ovarian tumors. By means of negative ion fast atom bombardment mass spectrometry (FABMS) and gas-liquid chromatography(GLC), the major sulfolipid in mucinous cystadenocarcinoma was confirmed as I^3SO_3-GalCer with N-cerebronyl phytosphingosine, rather than I^3SO_3-GalCer with N-nonhydroxy fatty acyl sphingosine, the major molecular species in the other ovarian cancers.2. a Monoclonal Antibody, MRG-1:A murine monoclonal antibody, MRG-1, established by immunizing mouse with human ovarian carcinoma-derived cells, reacted preferentially with the cells' Golgi complexes and microsome fractions, but not with the plasma membranes, whereas the antigen was distributed in the intercellular matrix of normal human cervical epithelium. The antigen was identified as neutral GSLs with blood group A-determinant by TLC-immunostaining. Using FABMS and NMR, the structure was found to be ceramide-Glc4-Gal3-GlcNAc4-Gal(2-Fuc)3-GalNAc3-Gal(2-Fuc)3-GalNAc, type IIIA GSLs.3. Galactosyltransferase Associated with Tumor (GAT):GAT was studied clinically in ovarian cancer patients. The GAT positive rate for different histologic types of ovarian carcinoma was relatively high for each type, e. g., 55.0% for clear cell carcinoma and 66.7% for endometrioid adenocarcinoma. In patients with benign diseases, in particular endometriosis, the GAT positive rate was lower than the positive rate with any other simultaneously definite markers. These results indicate the usefulness of GAT in distinguishing malignant from benign ovarian tumors.

1.各种人类卵巢肿瘤的鞘糖脂(GSL)：在人类卵巢肿瘤、粘液性囊腺癌、浆液性囊腺癌和透明细胞腺癌中，粘液性囊腺癌表现出独特的GSL组成。粘液性囊腺癌中超过 90% 的酸性 GSL 由硫脂组成，在正常卵巢中很少检测到，在其他类型的卵巢肿瘤中浓度低于 40%。通过负离子快原子轰击质谱（FABMS）和气液色谱（GLC），证实粘液性囊腺癌中的主要硫脂为I^3SO_3-GalCer与N-脑酰基植物鞘氨醇，而不是I^3SO_3-GalCer与N-非羟基脂肪酰基鞘氨醇（N-非羟基脂肪酰基鞘氨醇）。其他卵巢癌2． a 单克隆抗体，MRG-1：鼠单克隆抗体，MRG-1，通过用人卵巢癌来源的细胞免疫小鼠而建立，优先与细胞的高尔基复合体和微粒体部分反应，但不与质膜反应，而抗原分布在正常人宫颈上皮的细胞间基质中。通过TLC免疫染色，该抗原被鉴定为具有A型血型决定簇的中性GSL。使用FABMS和NMR，发现结构为神经酰胺-Glc4-Gal3-GlcNAc4-Gal(2-Fuc)3-GalNAc3-Gal(2-Fuc)3-GalNAc，IIIA型GSLs。3。肿瘤相关半乳糖基转移酶 (GAT)：在卵巢癌患者中对 GAT 进行了临床研究。卵巢癌不同组织学类型的GAT阳性率相对较高，例如：例如，透明细胞癌为 55.0%，子宫内膜样腺癌为 66.7%。在良性疾病，特别是子宫内膜异位症患者中，GAT阳性率低于任何其他同时明确的标志物的阳性率。这些结果表明 GAT 在区分恶性和良性卵巢肿瘤方面的有用性。