Functional recovery after nerve transplantation to optic nerve

视神经神经移植术后功能恢复

• 批准号: 13671837
• 负责人: SASAKI Hitoshi
• 金额: $ 2.62万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671837/
• 关键词: Functional Recovery; Motion Stimulus; Nerve Transplantation; Shuttle-Box; Avoidance Learning; Visual System; Sine-wave Gratings; Rat; 先行刺激抑制効果; α-クロラロース; 驚愕反応; 驚愕刺激; 白色雑音

Reconstruction of visual pathway can be achieved by the peripheral nerve transplantation to the cut end of the optic nerve. It has been reported that the restored visual pathway can conduct some visual information such as light-dark. In this research we have tried to evaluate motion information can be processed by the restored visual system, using prepulse inhibition with a motion stimulus as a prepulse. To obtain this result, animal's visual field should be fixed. Thus we have tried to record prepulse inhibition under alpha-chloralose anesthesia. However, clear prepulse effects could not be obtained in this condition in normal control rats. Next, we have tried to evaluate detection of motion stimulus using shuttle-box avoidance task. A motion of sine-wave gratings was used as a conditioned stimulus. Unconditioned stimulus was a shock from the floor of the box. Clear learning curves have been obtained with a motion speed of 40, 20, 1.5,0.5 deg/s, showing avoidance rate of 55-70% at the 5th day of training. Nine transplanted rats have been tested using prepulse inhibition with a white LED as a prepulse. One rat showed a tendency of inhibition effect. This rat was tested in the shuttle-box using a motion stimulus of 20 deg/s. Avoidance rate increased as progress of training sessions. After the test with 20 deg/s, same training has been done with a motion speed of 40 deg/s. However, no learning effect was observed. Another transplanted rat, showing no prepulse effect to LED prepulse was tested as a control for shuttle-box task. No improvement of avoidance rate has been observed. These results suggest that some transplanted rat might be able to detect motion

将周围神经移植到视神经的切端可以重建视觉通路。有报道称，恢复后的视觉通路可以传导光-暗等视觉信息。在本研究中，我们尝试用运动刺激作为预脉冲的预脉冲抑制来评估恢复视觉系统对运动信息的处理能力。要得到这个结果，动物的视野必须是固定的。因此，我们尝试在-氯醛麻醉下记录脉前抑制。然而，正常对照大鼠在这种情况下没有明显的预搏作用。接下来，我们尝试使用穿梭盒回避任务来评估运动刺激的检测。用正弦波光栅运动作为条件刺激。无条件刺激是来自盒子底部的电击。在运动速度为40、20、1.5、0.5度/秒时，获得了清晰的学习曲线，训练第5天的回避率为55-70%。9只移植大鼠进行了用白光LED作为预脉冲的预脉冲抑制试验。一只大鼠表现出抑制作用的倾向。用20度/秒的运动刺激在梭箱中对这只大鼠进行测试。回避率随着训练时间的增加而增加。在以20度/秒的速度进行测试后，以40度/秒的速度进行相同的训练。然而，没有观察到学习效果。另一只移植大鼠对LED预脉冲无预脉冲效应，作为穿梭箱任务的对照。没有观察到回避率的改善。这些结果表明，一些移植的大鼠可能能够检测到运动

项目成果

Goto, K., Ueki, A.Iso, H., Morita, Y.: "Reduced prepulse inhibition in rats with entorhinal cortex lesions"Behav.Brain Rest. 134. 201-207 (2002)

Goto, K.、Ueki, A.Iso, H.、Morita, Y.：“内嗅皮层损伤的大鼠中前脉冲抑制减少”Behav.Brain Rest。

佐々木仁: "色彩刺激に対する脳波変化"日本心理学会第66回大会論文集. 367 (2002)

佐佐木仁：“对颜色刺激的脑电波变化”，日本心理学会第 66 届年会记录 367（2002）。

磯 博行, 佐々木 仁他: "心理学実験ノート"心理学実験ノート編纂委員会(編)二弊社. 191 (2001)

Hiroyuki Iso、Hitoshi Sasaki 等：《心理学实验笔记》心理学实验笔记编译委员会（编）2 我们公司 191（2001）。

Sasaki, H., et al.: "Hemispheric lateralization in detection of color stimuli."Neuroscience Research Supple.. 26. s116 (2002)

Sasaki, H., et al.：“颜色刺激检测中的半球偏侧化。”Neuroscience Research Supple.. 26. s116 (2002)

Sasaki, H., Fukuda, Y.: "Prepulse inhibition in the rat anesthetized with α -chloralose"Neuroscience Research Supple.. 25. s116 (2002)

Sasaki, H., Fukuda, Y.：“用α-氯醛糖麻醉大鼠的前脉冲抑制”神经科学研究补充.. 25. s116 (2002)