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Basic Research for Allograft of Omentum

同种异体大网膜移植的基础研究

基本信息

批准号：
13671879
负责人：
KAMEI Yuzuru
金额：
$ 2.18万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671879/
关键词：
omentum allograft skin flap microsurgery wound healing angiogenesis 胃壁血管内皮細胞

项目摘要

Microsurgical reconstruction using the omental flap has recently made marked progress. However, we cannot use the omental flap in cases which it was used or resected before. So allograft of the omental flap will be useful. We report the basic research for the allograte of omentum. At first, what is of great importance knows the tolerance limits for omental ischemic time. For this reason, we present an experimental study in which we examined the condition of the omentum after hemostasis of various durations. The omental flaps were elevated based on gastroepiploic vessels in adult Wistar rats. Ischemic omental models were made hourly from 1 to 6 hours in order to establish a standard for ischemic time limit. After 10 minutes, the omental flaps were examined macroscopically as well as microscopically. The recovery process of 2-hour ischemic omental models was examined at 10 minutes, 1 day, 2 days, 3 days, and 7 days after declamping, to establish a morphological standard for the evaluation of omental recovery. Ischemic omental models of 2 hours were similar to normal omentum macroscopically and microscopically. The ischemic omental time limit was estimated to range from 3 to 4 hours. A histological specimen of 2-hour ischemic model examined 3 days after declamping showed vascularization and infiltration of fibroblasts. A histological specimen of 2-hour model examined 7 days after declamping showed remodeled collagen fiber partially. At second step, wound healing using the omental flap in nude mouse was examined, because immunosuppressant is used at allograft. The wound covered with omentum was almost healed in normal skin and the wound without omentum was healed such as a scar. The omental flap was useful for wound healing in nude mouse. Our research was a first step for the allograft of omentum.

显微外科重建网膜皮瓣最近取得了显着的进展。然而，我们不能使用网膜瓣的情况下，它是使用或切除前。因此，同种异体大网膜瓣移植将是有益的。本文报道了大网膜移植术的基础研究。首先，了解网膜缺血时间的耐受限度是非常重要的。出于这个原因，我们提出了一项实验研究，我们检查了不同持续时间的止血后网膜的状况。以胃网膜血管为蒂，将大网膜瓣抬高。每隔1 ~ 6小时制作大网膜缺血模型，建立缺血时限标准。10分钟后，肉眼和显微镜检查网膜瓣。在开放后10 min、1 d、2 d、3 d、7 d观察缺血2 h大网膜模型的恢复过程，建立评价大网膜恢复的形态学标准。缺血2 h大网膜模型大体和显微镜下与正常大网膜相似。缺血性网膜时限估计为3至4小时。在松开夹钳3天后检查的2小时缺血模型的组织学标本显示血管化和成纤维细胞浸润。开放后7天检查的2小时模型的组织学标本显示部分重塑的胶原纤维。在第二步中，检查了在裸鼠中使用网膜瓣的伤口愈合，因为在同种异体移植物中使用了免疫抑制剂。有网膜覆盖的创面在正常皮肤下基本愈合，无网膜覆盖的创面愈合如瘢痕。大网膜瓣对裸鼠创面愈合有促进作用。本研究为同种异体大网膜移植迈出了第一步。