Ultra-morphological and chemical analysis of lased dentin for the adhesion promotion of resinous materials

用于促进树脂材料粘附的激光牙本质的超形态和化学分析

• 批准号: 13671999
• 负责人: FUJITANI Morioki
• 金额: $ 1.98万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671999/
• 关键词: Er : YAG laser; CO_2 laser; Adhesive resin; Dentin bonding; Interface of adhesion; Morphological study; Analytical-chemical study; Adhesion promotion; Er : YAGレーザー; CO2レーザー; 接着機構

This study was carried out to investigate the ultra-morphological and chemically-analytical changes of the dentin surface induced by Er : YAG or CO_2 laser irradiation for the purpose of the adhesion promotion of resinous materials to lased dentin.Labial surface of freshly extracted bovine tooth was ground to expose flat dentin. The dentin surfaces were then polished with 600-grid SiC paper and were irradiated by Er : YAG laser (Erwin^R, 60mJ/pulse, 10pps) or CO_2 laser (Topal^R, 1W, 0.3 sec). The superficial layer of the lased dentin was ultra-morphologically examined by atomic force microscopy (AFM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The surface characteristics of lased dentin were chemically analyzed using X-ray Photoelectron Spectroscopy (XPS).The SEM and AFM observation revealed that the smear layer was not produced on the lased dentin surface, where the structural defects such as micro cracks and stratified structure like fish scales were observed. By the TEM observations of non-decalcified specimens, such morphological changes as observed in the acid-etched dentin surface could not be detected in the subsurface layer of the lased dentin. Furthermore, the amorphous matrix substances of 10-15μm thickness, which consisted of denatured dentin collagen, were superficially observed. The analysis of XPS wide-scan spectrum of lased dentin surface showed the intensity of both C1s and N1s peaks obviously decreased. The SEM examination of resin-dentin interface revealed that the degenerated layer of 10-12μm thickness was observed beneath the hybrid layer.It was suggested that both the structural defects and the denatured layer affect the adhesive properties of resinous materials to the Er : YAG or CO_2 laser-irradiated dentin.

本研究以新鲜牛牙唇面为材料，观察Er：YAG激光和CO_2激光照射牙本质后牙本质表面的超微结构和化学成分的变化，探讨Er：YAG激光和CO_2激光对牙本质表面粘附的影响。牙本质表面用600格SiC纸抛光，用Er：YAG激光（Erwin^R，60 mJ/pulse，10 pps）或CO_2激光（Topal^R，1 W，0.3sec）照射。用原子力显微镜（AFM）、扫描电镜（SEM）和透射电镜（TEM）观察激光照射后牙本质表层的超微结构。采用X射线光电子能谱（XPS）对激光处理后牙本质表面进行化学分析，SEM和AFM观察发现激光处理后牙本质表面未产生玷污层，表面存在微裂纹和鱼鳞状分层结构等结构缺陷。未脱钙标本的透射电镜观察表明，酸蚀牙本质表面的形态变化在激光照射牙本质的次表层没有表现出来。此外，表面还观察到厚度为10-15μm的由变性牙本质胶原组成的无定形基质物质。XPS宽扫描谱分析表明，激光处理后牙本质表面的C1 s峰和N1s峰强度明显降低。树脂-牙本质界面的扫描电镜观察显示，在混合层下有厚度为10-12μm的变性层，这表明混合层的结构缺陷和变性层都影响了树脂材料与Er：YAG或CO_2激光照射牙本质的粘接性能。

项目成果

Takahiro Harima: "Adhesive properties of resinous materials to lased dentin. Part 5 Interfacial ultramorphology between adhesives and lased dentin"The Japanese Journal of Conservative Dentistry. 44-Spring issue, 12. (2001)

Takahiro Harima：“树脂材料与激光牙本质的粘合特性。第 5 部分粘合剂与激光牙本质之间的界面超形态”日本保守牙科杂志。

Takahiro Harima: "Surface Characteristics of Laser (Er : YAG)-irradiated Dentin"Adhesive Dentistry. 19-4. 348 (2002)

Takahiro Harima：“激光（Er：YAG）照射牙本质的表面特性”粘合牙科。

播磨 貴裕: "レーザー処理象牙質におけるレジンの接着 第5報 接着界面の超微細構造について"日本歯科保存学雑誌. 44春季特別号. 12 (2001)

Takahiro Harima：“激光处理牙本质上的树脂粘附第 5 部分：粘附界面的超细结构”日本保守牙科杂志 44 春季特刊（2001 年）。

T.Harima: "Surface Characteristics of Laser (Er:YAG) -irradiated Dentin"接着歯学. 19巻4号. 348 (2002)

T. Harima：“激光 (Er:YAG) 照射的牙本质的表面特性”，《粘合剂牙科》，第 19 卷，第 4. 348 期（2002 年）

Morioki Fujitani: "Does Er : YAG or CO2 laser ablation of dentin affect the adhesive properties of resin bonding systems?"Excerpta Medica International Congress Series. 1683, in press. (2003)

Morioki Fujitani：“Er：YAG 或 CO2 激光烧蚀牙本质会影响树脂粘合系统的粘合性能吗？”Excerpta Medica 国际会议系列。