Evaluation of Direct Adhesive Pulp Capping through Wound Healing and Hard Tissue Regeneration

通过伤口愈合和硬组织再生直接粘合盖髓的评估

• 批准号: 08672192
• 负责人: FUJITANI Morioki
• 金额: $ 1.41万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672192/
• 关键词: Animal study; Adhesive resin; Direct pulp capping; Pulpal response; Dentin bridge; Fluorescent dye; Labelling of mineralizing tissue; Ultramorphology; 病理組織学

The objective of this study was to evaluate adhesive resins for direct pulp capping through wound healing and hard tissue regeneration. The exposed cavities prepared in Japanese monkeys were placed directly with Bis-GMA (BG) or 4-META/MMA-TBB (MMA) resin. The response of pulpal tissue and ultramorphology of the resultant resin-pulp interface were analyzed, and also the formation of dentin bridge was investigated through multiple fluorescent labelling of mineralizing tissue. BG exhibited primay healing within 3-7 days. Hard tissue regeneration occurred after 7-14 days, and dentin bridge was formed with time with secondary dentin by stimulation of the cavity preparation. At the initial stage of mineralization, labelling lines exhibited scalloping, showing irregular formation of dentin bridge. Both ends of each line were identical, demonstrating thin layrs of reparative dentin with the same ends were placed and stratified. At 90 days, a complete dentin bridge appeared beneath the resin wi … More thout gap formation. A thin interfacial layr with proteoglycan matrix was produced at the front of the bridge, whose structure was similar to that of the reversal line which usually appeared in the remodeling process of bone tissue. In the MMA specimen, a region with many acute inflammatory cells was found beneath the resin at 3 days. Many macrophages surrounded it, indicating presumably that it had not been recognized as their own tissue and had turned to phagocytable. This region is considered to be th sof tissue hybrid layr in which the resin penetrated or diffused into pulp tissue. An almost complete bridge was produced without any interfacial layr. A thin layr of pulp tissue remained between the resin and bridge, demonstrating that the hybrid might delay the prompt healing. It was concluded that the process of healing and hard tissue regeneration was different according to the capped bonding system, and that the multiple fluorescent labelling technique was effective for the dynamic analysis of pulpal healing. Less

本研究的目的是评估通过伤口愈合和硬组织再生直接覆盖牙髓的粘接树脂。用Bis-GMA （BG）或4-META/MMA- tbb （MMA）树脂直接置入日本猴的暴露腔体。分析了矿化组织对牙本质组织的响应和树脂-牙髓界面的超微形貌，并通过矿化组织的多次荧光标记研究了牙本质桥的形成。BG在3-7天内初步愈合。7 ~ 14 d后出现硬组织再生，牙本质桥随着时间的推移在预备腔刺激下与次牙本质形成。矿化初期标记线呈扇形，牙本质桥形成不规则。每条线的两端相同，表明修复牙本质的端部相同的薄层被放置和分层。在第90天，树脂下出现了一个完整的牙本质桥，并且没有形成间隙。在骨桥前部形成一层薄的蛋白聚糖基质界面层，其结构类似于骨组织重塑过程中常见的逆转线。在MMA标本中，在3天的树脂下发现一个有许多急性炎症细胞的区域。许多巨噬细胞包围着它，推测这表明它没有被识别为自己的组织，已经变成了可吞噬的组织。这个区域被认为是软组织杂交层，树脂在其中渗透或扩散到牙髓组织。一个几乎完整的桥没有任何界面层。树脂和牙桥之间有一层薄薄的牙髓组织，这表明这种混合物可能会延迟伤口的迅速愈合。结果表明，不同的盖顶结合体系对牙髓愈合和硬组织再生的影响不同，多重荧光标记技术对牙髓愈合的动态分析是有效的。少

项目成果

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Shuichi Sonoda：“暴露的牙髓对各种牙科水泥的组织反应”，日本保守牙科杂志 39（春季特刊）。

Sonoda, Hidekazu: "Tissue Reaction of Exposed Dental Pulp to Various Dental Cements" The Japanese Journal of Conservative Dentistry. 39-Special issue. 59 (1996)

Sonoda，Hidekazu：“暴露牙髓对各种牙科水泥的组织反应”日本保守牙科杂志。

Sonoda, Hidekazu: "Tissue reaction of exposed dental pulp to various dental cements" Proceedings of the First International Academic Conference on Dental Pulp Biology. 19-20 (1996)

Sonoda，Hidekazu：“暴露牙髓对各种牙科水泥的组织反应”第一届国际牙髓生物学学术会议论文集。

Kitasako, Yuuichi: "Direct pulp capping with several adhesive resin systems" Proceedings of the First International Academic Conference on Dental Pulp Biology. 21-22 (1996)

Kitasako，Yuuichi：“用几种粘合树脂系统直接盖髓”第一届国际牙髓生物学学术会议论文集。

藤井 理史: "マレイン酸を含有するetchantとself-etching primerを用いた接着システムの直接覆髄材としての評価" 日本歯科保存学会1997年度春季学会(106回)発表予定. 40(春季特別号). (1997)

Satoshi Fujii：“使用含马来酸蚀刻剂和自蚀刻底漆作为直接盖髓材料的粘合剂系统的评估”预定在日本保守牙科学会 1997 年春季会议上发表（第 106 期）（春季特刊）（ 1997）