Evaluation of Direct Adhesive Pulp Capping through Wound Healing and Hard Tissue Regeneration

本研究的目的是通过伤口愈合和硬组织再生来评估用于直接盖髓的粘合树脂。在日本猴中制备的暴露腔直接用Bis-GMA（BG）或4-Meta/MMA-TBB（MMA）树脂放置。分析牙髓组织的反应和树脂-牙髓界面的超微形态，并通过矿化组织的多重荧光标记研究牙本质桥的形成。BG在3-7天内表现出早期愈合。术后7-14 d开始硬组织再生，随着时间的推移，牙本质桥形成，牙本质桥在窝洞预备物的刺激下形成次生牙本质。矿化初期标记线呈扇形，显示牙本质桥不规则形成。每条线的两端相同，表明具有相同端部的修复性牙本质薄层被放置和分层。90天时，树脂下出现完整的牙本质桥， ...更多信息 没有间隙形成。在骨桥前端形成一薄层蛋白多糖基质界面层，其结构类似于骨组织改建过程中常见的逆转线。在MMA样本中，在3天时在树脂下方发现具有许多急性炎症细胞的区域。许多巨噬细胞包围着它，这大概表明它没有被识别为自己的组织，而是变成了可吞噬的。该区域被认为是树脂渗透或扩散到牙髓组织中的组织混合层。制作了几乎完整的桥，没有任何界面层。在树脂和桥之间保留了一薄层牙髓组织，表明混合物可能会延迟快速愈合。结论：不同的粘接系统，其愈合和硬组织再生的过程不同，多重荧光标记技术可有效地用于牙髓愈合的动态分析。少

The objective of this study was to evaluate adhesive resins for direct pulp capping through wound healing and hard tissue regeneration. The exposed cavities prepared in Japanese monkeys were placed directly with Bis-GMA (BG) or 4-META/MMA-TBB (MMA) resin. The response of pulpal tissue and ultramorphology of the resultant resin-pulp interface were analyzed, and also the formation of dentin bridge was investigated through multiple fluorescent labelling of mineralizing tissue. BG exhibited primay healing within 3-7 days. Hard tissue regeneration occurred after 7-14 days, and dentin bridge was formed with time with secondary dentin by stimulation of the cavity preparation. At the initial stage of mineralization, labelling lines exhibited scalloping, showing irregular formation of dentin bridge. Both ends of each line were identical, demonstrating thin layrs of reparative dentin with the same ends were placed and stratified. At 90 days, a complete dentin bridge appeared beneath the resin wi … More thout gap formation. A thin interfacial layr with proteoglycan matrix was produced at the front of the bridge, whose structure was similar to that of the reversal line which usually appeared in the remodeling process of bone tissue. In the MMA specimen, a region with many acute inflammatory cells was found beneath the resin at 3 days. Many macrophages surrounded it, indicating presumably that it had not been recognized as their own tissue and had turned to phagocytable. This region is considered to be th sof tissue hybrid layr in which the resin penetrated or diffused into pulp tissue. An almost complete bridge was produced without any interfacial layr. A thin layr of pulp tissue remained between the resin and bridge, demonstrating that the hybrid might delay the prompt healing. It was concluded that the process of healing and hard tissue regeneration was different according to the capped bonding system, and that the multiple fluorescent labelling technique was effective for the dynamic analysis of pulpal healing. Less