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A Study on the Application to the Pulp Capping Agent of a DNA Film

DNA薄膜盖髓剂的应用研究

基本信息

批准号：
13672018
负责人：
YAMADA Kazuhiko
金额：
$ 2.56万
依托单位：
Fukuoka Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672018/
关键词：
DNA-Film Vital pulpotomy Pulp Capping Hydroxyapatite 覆髄 BMP アパタイトビーグル犬電顕的観察

项目摘要

Presently, vital pulpotomy using calcium hydroxide is widely performed in general clinical treatment, and dentin bridge formation is considered an indication of its success. However, since calcium hydroxide is a strong alkali, it has been reported to cause extensive necrosis in pulp tissue. Furthermore, the formation of new, hard tissue such as a dentin bridge is considered to indicate that toxic stimulation has been applied to the pulp. If pulp tissue could be preserved with less damage through the use of a biocompatible material, it would be considered to be extremely significant. Therefore, we investigated the use of artificial lipid-modified DNA, which has been reported to be moderately biodegradable, non-antigenic, and antibiotic, as such a material. Fukushima et al. showed that artificial lipid-modified DNA is a promising biodegradable biological material, and it is expected to be applied to dentistry. In this study, we evaluated its use as a pulp dressing material after vital pulpotomy. Since preliminary experiments revealed problems with the manipulability of DNA film itself, we applied uncoated and DNA-coated hydroxyapatite to pulpotomy surfaces of the bilateral upper and lower canine teeth in beagles and histopathologically examined pulp tissue changes. In the pulp treated with DNA-coated hydroxyapatite, inflammatory cell infiltration and calcification were milder than in those treated with uncoated hydroxyapatite. These findings suggest that DNA is not toxic to pulp tissue and even exhibits an anti-inflammatory activity. Moreover, as DNA can intercalate antibiotics and osteogenesis inducers, it may be applied in the future as a pulp dressing agent.

目前，氢氧化钙活髓切断术已广泛应用于临床，而牙本质桥的形成被认为是其成功的标志。然而，由于氢氧化钙是一种强碱，据报道，它会导致牙髓组织广泛坏死。此外，新的硬组织如牙本质桥的形成被认为表明毒性刺激已被施加到牙髓。如果能够通过使用生物相容性材料以较小的损伤保存牙髓组织，则将被认为是非常重要的。因此，我们研究了使用人工脂质修饰的DNA，据报道，它是适度生物降解，非抗原性和抗生素，作为这样的材料。福岛等研究表明，人工脂质修饰的DNA是一种很有前途的可生物降解的生物材料，有望应用于牙科。在这项研究中，我们评估了它作为活髓切断术后的牙髓敷料的用途。由于初步的实验发现的问题与DNA膜本身的可操作性，我们应用未涂层和DNA涂层的羟基磷灰石的双边上，下犬齿在比格犬和组织病理学检查牙髓组织的变化。在牙髓治疗与DNA涂层羟基磷灰石，炎症细胞浸润和钙化是温和的比那些治疗与未涂层羟基磷灰石。这些发现表明DNA对牙髓组织没有毒性，甚至表现出抗炎活性。此外，由于DNA可以嵌入抗生素和成骨诱导剂，它可能在未来作为牙髓敷料剂应用。