Establishment of New Evaluation for Periodontal Disease, Research for Risk Factors in Human Saliva

本研究的目的是检测整个唾液样本中组蛋白5和Cu/Zn超氧化物歧化酶（Cu/Zn SOD）的含量，这些唾液样本被分为10岁至70岁年龄段（n=287），并评估个体牙周病发展的风险。采用单克隆抗体酶联免疫吸附测定（ELISA）试剂盒检测组蛋白5和Cu/Zn超氧化物歧化酶的含量。结果如下：1)全唾液组蛋白5和Cu/Zn SOD的含量（μg/mg唾液总蛋白）存在较大的个体差异；2)10 ~ 19岁组蛋白5的平均含量为5.3,70多岁组蛋白5的含量有降低的趋势，且呈2.5的升高趋势，而Cu/Zn SOD的平均含量在各年龄组间差异无统计学意义（p<0.05，方差分析）；3) 40岁以上健康人群与40岁以上牙周病患者组蛋白5和Cu/Zn超氧化物歧化酶（SOD）的均值差异有统计学意义（p<0.05，Mann-Whitney检验）。这项研究的结果表明，有可能在牙科诊所的椅子旁使用一种新的筛查测试试剂盒来诊断牙周病。

The purpose of this study was to examine the quantities of histatin 5 and Cu/Zn super oxide dismutase (Cu/Zn SOD) in whole saliva sample from subjects divided into age groups ranging from 10 years to 70's (n=287) and to evaluate the individual risk for development of periodontal disease. The quantities of histatin 5 and Cu/Zn super oxide dismutase were determined hy using an enzyme-linked immunosorbent assay (ELISA) kit with a monoclonal antibody.The results were as follows : 1)the quantities (μg/mg of total salivary protein) of histatin 5 and Cu/Zn SOD in whole saliva showed large individual differences, 2)the mean quantities of histatin 5 in subjects aged 10-19 years was 5.3, and it showed a tendency to decrease with advance of the value being 2.5 in the 70's age group, whereas the mean quantity of Cu/Zn SOD showed no significant difference (p<0.05,ANOVA) between age groups, and 3) the mean values of the quantity of both histatin 5 and Cu/Zn SOD showed a significant difference (p<0.05,Mann-Whitney test) in healthy people aged over 40 years in subjects aged over 40 years who had periodontal disease.The results of this study suggest that it is possible to a new screening test kit that can be used at the chairside in a dental clinic for diagnosis ofperiodontal disease.