Establishment of New Evaluation for Periodontal Disease, Research for Risk Factors in Human Saliva

建立牙周病新评估方法，研究人类唾液中的危险因素

• 批准号: 13672136
• 负责人: KANEHIRA Takashi
• 金额: $ 2.62万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672136/
• 关键词: saliva; histatin; SOD; individual difference; periodontal disease; risk factor; リスクファクター; SOD

The purpose of this study was to examine the quantities of histatin 5 and Cu/Zn super oxide dismutase (Cu/Zn SOD) in whole saliva sample from subjects divided into age groups ranging from 10 years to 70's (n=287) and to evaluate the individual risk for development of periodontal disease. The quantities of histatin 5 and Cu/Zn super oxide dismutase were determined hy using an enzyme-linked immunosorbent assay (ELISA) kit with a monoclonal antibody.The results were as follows : 1)the quantities (μg/mg of total salivary protein) of histatin 5 and Cu/Zn SOD in whole saliva showed large individual differences, 2)the mean quantities of histatin 5 in subjects aged 10-19 years was 5.3, and it showed a tendency to decrease with advance of the value being 2.5 in the 70's age group, whereas the mean quantity of Cu/Zn SOD showed no significant difference (p<0.05,ANOVA) between age groups, and 3) the mean values of the quantity of both histatin 5 and Cu/Zn SOD showed a significant difference (p<0.05,Mann-Whitney test) in healthy people aged over 40 years in subjects aged over 40 years who had periodontal disease.The results of this study suggest that it is possible to a new screening test kit that can be used at the chairside in a dental clinic for diagnosis ofperiodontal disease.

本研究的目的是检测S(n=287)10~70岁年龄组唾液标本中组蛋白5和铜/锌超氧化物歧化酶的含量，以评估个体患牙周病的危险性。用单抗酶联免疫吸附测定试剂盒检测唾液中组蛋白5和铜/锌超氧化物歧化酶的含量。结果表明：1)唾液中组蛋白5和铜/锌超氧化物歧化酶的含量(μg/mg)存在较大的个体差异；2)10~19岁年龄组的平均含量为5.3，70岁的S年龄组随年龄增大呈下降趋势，而铜/锌超氧化物歧化酶的平均值无显著差异(p&lt)；年龄组间比较差异有统计学意义(P<0.05，ANOVA)。3)40岁以上健康人群中，40岁以上牙周病患者血清中组蛋白5和铜/锌超氧化物歧化酶含量的均值有显著差异(P&lt；0.05，Mann-Whitney检验)。