Study of Angiostatin Genetherapy for Oral Cancer

血管抑制素基因治疗口腔癌的研究

• 批准号: 13672127
• 负责人: USHAKU Lee
• 金额: $ 1.98万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672127/
• 关键词: Angiostatin; Oral Cancer; Gene Therapy; Angiogenesis; アンジオスタチン; 扁平上皮癌; 遺伝子治療

Antiangiogenic tumor therapies have recently attracted intense interest because of their direct endothelial targeting and the absence of drug resistance. To achieve tumor regression and tumor dormancy, antiangiogenic agents need to be administered. Local antiangiogenic gene therapy for cancer offers a potential way to achieve sustained therapeutic release of antiangiogenic substances. As a step toward this goal, we used liposomes complexed to angiostatin cDNA and targeted to human squamous cell carcinoma cell lines in vivo. Tumor cells expressing angiostatin after local gene transfer showed markedly reduced vascularity and contained many apoptotic tumor cells. These results demonstrate the potential utility of liposome-derived angiostatin for adjuvant therapy of oral cancer in humans.

抗血管生成肿瘤治疗由于其直接的内皮靶向作用和无耐药性，最近引起了人们的极大兴趣。为了实现肿瘤消退和肿瘤休眠，需要施用抗血管生成剂。肿瘤局部抗血管生成基因治疗为实现抗血管生成物质的持续治疗释放提供了一种潜在的途径。作为实现这一目标的一步，我们使用脂质体复合血管抑素cDNA和靶向人鳞状细胞癌细胞系在体内。局部基因转移后表达血管抑素的肿瘤细胞显示血管明显减少，并含有许多凋亡肿瘤细胞。这些结果证明了脂质体衍生的血管抑素用于人类口腔癌辅助治疗的潜在效用。

项目成果

Matsumoto G. et al.: "Growth of human squamous cell carcinoma xenografts in mice is inhibited by local angiostatin gene therapy"Oral Oncology. 38. 543-548 (2002)

Matsumoto G.等人：“局部血管抑制素基因疗法抑制小鼠体内人鳞状细胞癌异种移植物的生长”口腔肿瘤学。

Ohmi Y., et al.: "Tumor growth inhibited by the expression of endostatin cDNA in murine squamous cellcarcinoma"The Bulletin of Kanagawa dental college. 29(2). 67-74 (2001)

Ohmi Y.等人：“小鼠鳞状细胞癌中内皮抑素cDNA表达抑制肿瘤生长”神奈川牙科学院通报。

Matsumoto G., et al.: "Growth of fuman squamous cell carcinoma xenografts in mice is inhibited by local angiostatin gene therapy"Oral Oncoklogy. 38. 543-548 (2002)

Matsumoto G.等人：“局部血管抑制素基因疗法抑制小鼠福曼鳞状细胞癌异种移植物的生长”口腔肿瘤学。

Ohmi Y., et al.: "Tumor growth inhibited by expression of endostatin cDNA in murine squamous cell carcinoma"The Bulletin of Kanagawa dental college. 29(2). 67-74 (2001)

Ohmi Y.等人：“小鼠鳞状细胞癌中内皮抑素cDNA表达抑制肿瘤生长”神奈川牙科学院通报。