Elucidation of invasive andmetastatic mechanism in oral cancer for application to molecular based diagnosisand gene therapy

阐明口腔癌的侵袭和转移机制，应用于分子诊断和基因治疗

• 批准号: 21249088
• 负责人: TAKATA Takashi
• 金额: $ 29.2万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21249088/
• 关键词: 口腔癌; 浸潤・転移; microRNA; 浸潤; 転移; 血管新生; 微小環境; MMP10; MMP13

To elucidate invasive and metastatic mechanism in oral cancer for application to molecular based diagnosis and gene therapy, we conducted research in accordance with our projects. As a result, we identified Wnt5B and MMP10 as promoter of , microRNA-203 as suppressor of tumor invasion, Periostin as promoter of lymphangiogenesis, MMP-13 as promoter of angiogenesis. Additionally, we attempted to apply Periostin to Elisa for serum of oral cancer patients. Interestingly, we found upregulation of Periostin correlated with lymphonode metastasis status. Therefore, we showed Periostin could be a useful for molecular diagnostic marker in oral cancer.

为了阐明口腔癌的侵袭转移机制，为口腔癌的分子诊断和基因治疗提供理论依据，我们结合课题组开展了相关研究。结果表明，Wnt 5 B和MMP 10是肿瘤侵袭的启动子，microRNA-203是肿瘤侵袭的抑制子，Periostin是淋巴管生成的启动子，MMP-13是血管生成的启动子。此外，我们尝试将Periostin应用于口腔癌患者血清的Elisa。有趣的是，我们发现Periostin的上调与淋巴结转移状态相关。因此，我们认为Periostin可能是一个有用的口腔癌分子诊断标志物。

项目成果

RUNX3 has an oncogenic role in head and neck cancer.

• DOI: 10.1371/journal.pone.0005892
• 发表时间: 2009-06-12
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Tsunematsu T;Kudo Y;Iizuka S;Ogawa I;Fujita T;Kurihara H;Abiko Y;Takata T
• 通讯作者: Takata T

Matrix Metalloproteinase-13 (MMP-13) Directly and Indirectly Promotes Tumor Angiogenesis

• DOI: 10.1074/jbc.m112.373159
• 发表时间: 2012-11-09
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Kudo, Yasusei;Iizuka, Shinji;Takata, Takashi
• 通讯作者: Takata, Takashi

Cyclooxygenase-2 inhibition causes antiangiogenic effects on tumor endothelial and vascular progenitor cells

• DOI: 10.1002/ijc.25976
• 发表时间: 2012-01-01
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Muraki, Chikara;Ohga, Noritaka;Hida, Kyoko
• 通讯作者: Hida, Kyoko

口腔癌の浸潤に関与する microRNA の網羅的解析

口腔癌侵袭相关microRNA的综合分析

• 发表时间: 2010
• 作者: 吉田真希;工藤保誠;高田 隆
• 通讯作者: 高田 隆

Incleased telomerase activity and hTERT expression in human salivary gland carcinomas

人唾液腺癌中端粒酶活性和 hTERT 表达增加

• 发表时间: 2011
• 期刊: Oncology Letter
• 作者: Shigeishi H;Sugiyama M;Tahara H;Ono S;Bhawal UK;Okura M;Kogo M;Shinohara M;Shindoh M;Shintani S;Hamakawa H;Takata T;Kamata N
• 通讯作者: Kamata N