Pathologic Studies on Induction of Craniomandibular Joint Arthritis is using in Rabbits Escherichia Coli Antigens

大肠杆菌抗原诱导兔颅颌关节关节炎的病理学研究

• 批准号: 13672132
• 负责人: KURITA Kenichi
• 金额: $ 1.41万
• 依托单位: SCHOOL OF DENTISTRY, AICHI-GAKUIN UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672132/
• 关键词: Arthritis in rabbit; enterobacterial antigens; Eschericia Coli : 014; 抗原誘導関節炎; rabbit cranio waudibular joint; enterobacterial anfigens; Escherichia coli 0:14; Experimental arthritis

Material and Method : While Japanese male rabbits were sensitized by Escherichia coli 0 : 14 and Freunds incomplete adjuvant. For the control group, rabbits were perfused with physiolosical saline and them purifyied water. On the other hand, for the experimental group, their lower cavity of the left temporomandibular joint (TMJ) were perfused with physiolosical saline and then infused with Escherichia coli 0 : 14 together with purifyied water. Several pieces of the latter were sacrificed on the 7^<th>, 14^<th>, 21st, 28^<th>, 35^<th>, and 56^<th> day after the above procedure for pathological observation by resecling the joint. Based on stainblocker's, Larsen's, Arthur's and Amaji's dassification, we invented a new classification method of our own for giving them comparison using scores.Results : (Control group) : No pathological variation was observed at the basic structure, although the carfilage layer got thinner with time. Score was class 0 throughout the entire period. (Experimental group) : 7^<th> day : An acute exudative inflammation was observed. 14^<th> day : The carfilage was destroyed owing to multiplication of pannus. 21st day : The lesion developed to reach the home, which indicates the transition to chronic inflammation. 28^<th> day : Further lesion development. Typical rheumatiod arthritis was observed, macrophage, plasma cell, conflent follicle of limphocyte, or formation of pannus. After this, it look a turn for the better to get cured gradually. Now we are following up about MMP-3 and obteopontin.As to the scores, arthritis destruction was promoted as described above, and it showed maximum point on the day of 28^<th>, which was (3.00+/-0.00).Remark : As the pathological process of the experimental group is just common to that of human TMJ, it is confimied that this experiment serves not only for illuminating the actual shape of rhemaloid arthritis and also the future therapeulic research.

材料和方法：用大肠杆菌0：14和弗氏不完全佐剂致敏日本雄性兔。对照组用生理盐水灌胃，净化水。实验组大鼠左侧颞下颌关节(TMJ)下腔灌入生理盐水后，加入0：14的大肠杆菌和纯净水。分别于术后第7、14、21、28、35、56天处死大鼠，取关节进行病理学观察。结果：(对照组)：基本结构未见病理改变，但随着时间的推移，纤维层变薄。在整个过程中，得分均为0级。(实验组)：第7天：观察到急性渗出性炎症。第14天：血管疙瘩增生性破坏。第21天：病变发展到家庭，这表明过渡到慢性炎症。第28天：病变进一步发展。典型的类风湿关节炎表现为巨噬细胞、浆细胞、淋巴细胞的内分泌滤泡或血管疙瘩的形成。在这之后，逐渐痊愈看起来是一个转机。现在我们正在对基质金属蛋白酶-3和桥桥素进行追踪。对于如上所述的促进关节炎破坏的评分，在第28天达到最大值，为(3.00+/-0.00)。备注：由于实验组的病理过程与人类TMJ的病理过程一样，证实本实验不仅有助于阐明类风湿性关节炎的实际形态，也有助于未来的治疗研究。

项目成果

水野 進: "ウサギにおける実験的大腸菌抗原誘導顎関節炎"愛知学院大学歯学会誌. 39(1). 83-96 (2001)

Susumu Mizuno：“实验性大肠杆菌抗原诱导的兔颌关节炎”爱知学院大学牙科学会杂志 39（1）（2001）。

水野 進: "ウサギにおける実験的大腸菌抗原誘導顎関節炎"愛知学院大学歯学会誌. 第39巻第1号. 83-96 (2001)

Susumu Mizuno：“实验性大肠杆菌抗原诱导的兔颌关节炎”，爱知学院大学牙科学会杂志，第 39 卷，第 1. 83-96 期（2001 年）。

SUSUMU MIZUNO: "Pathologic Studies on Induction of Craniomandibular Joint Arthritis Using in Rabbit Escherichia Coli Antigens"THE AICHI=GAKUIN JOURNAL OF DENTAL SCIENCE. 39(1). 83-96 (2001)

SUSUMU MIZUNO：“利用兔大肠杆菌抗原诱导颅颌关节关节炎的病理学研究”爱知=学院牙科杂志。