Fundamental examination of repeat structure of the Y chromosome STR locus

Y染色体STR位点重复结构的基础检查

• 批准号: 13672177
• 负责人: YAMADA Yoshihiro
• 金额: $ 1.22万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672177/
• 关键词: forensic medicine; personal identification; Paternity; Y-STR; repeat structure; direct sequencing; YSTR

Recently described Y short tandem repeat (Y-STR) polymorphisms can be analyzed as informative haplotypes, which are useful in the forensic field. In order to include these systems in our forensic routine, sequence analysis was conducted about the 7 kinds of range (the locus) of DYS19, DYS389 I, DYS389 II, DYS390, DYS391, DYS392 and DYS393.DYS391 showed the repetition of unique, simple unit. DYS389 I, DYS389 II, DYS390 and DYS393 showed the repetition that irregularly, 2 kinds of different units, were mixed each other. Furthermore, there was the insertion of a special arrangement in the font or middle of the unit which DYS19 and DYS392 were decide.We have investigated a case of disputed paternity where the alleged father was dead, using data from the surviving children for eight Y-STR loci (DYS19, DYS389 I, DYS389 II, DYS390, DYS391, DYS392, DYS393 and DYS385: two loci). Various alleles for each system (except DYS385) were sequenced. In the eight Y-STRs both the two legitimate children and a further additional child of the deceased putative father carried the same allele. The present study demonstrates the structure of Y-STRs and the power of Y-STR typing as a tool for solving this kind of case.

最近描述的Y短串联重复序列（Y-STR）多态性可以作为信息单倍型进行分析，这在法医学领域是有用的。为了将这些系统纳入法医学常规，对DYS 19、DYS 389 I、DYS 389 II、DYS 390、DYS 391、DYS 392和DYS 393的7种范围（位点）进行了序列分析。DYS 389 Ⅰ、DYS 389 Ⅱ、DYS 390和DYS 393表现为两种不同单位不规则地相互混合的重复。我们利用8个Y-STR基因座的数据，对一起父亲死亡的亲子关系纠纷进行了调查（DYS 19、DYS 389 I、DYS 389 II、DYS 390、DYS 391、DYS 392、DYS 393和DYS 385：两个基因座）。对每个系统（DYS 385除外）的各种等位基因进行测序。在8个Y-STR中，两个婚生子女和已故推定父亲的另一个子女携带相同的等位基因。本研究展示了Y-STR的结构和Y-STR分型作为解决此类案件的工具的力量。

项目成果

Yoshihiro Yamada: "Dental information (postmortem dental roentgenoloey, the typing of DNA from a tooth) plays a conclusive role in dental identification"Proceedings of the 16^<th> IAFS. 153-156 (2002)

Yoshihiro Yamada：“牙科信息（死后牙科 X 线检查，牙齿 DNA 分型）在牙科鉴定中发挥着决定性作用”第 16 届 IAFS 会议记录。

Yoshihiro Yamada: "Possibility of paternity testing using RFLP analysis on a very small amount of material"J Forens Odonto-Stomatol. 19. 1-4 (2001)

Yoshihiro Yamada：“使用 RFLP 分析对极少量材料进行亲子鉴定的可能性”J Forens Odonto-Stomatol。

Yoshihiro Yamada.: "Expression of paraoxonase isoform did not confer protection from acute sarin poisoning in the Tokyo subway terrorist attack"Int J Legal Med.. 115. 82-84 (2001)

Yoshihiro Yamada.：“在东京地铁恐怖袭击中，对氧磷酶异构体的表达并未赋予人们免受急性沙林中毒的保护”Int J Legal Med.. 115. 82-84 (2001)

Yoshihiro Yamada: "Expression of paraoxonase isoform did not confer protection from acute sarin poisoning in the Tokyo subway terrorist attack"International Journal of Legal Medicine. 115・2. 82-84 (2001)

Yoshihiro Yamada：“在东京地铁恐怖袭击中，对氧磷酶亚型的表达并未赋予人们免受急性沙林中毒的保护”，《国际法律医学杂志》115·2（2001）。

Hiroshi Ohira.: "Identification applying mitochondrial DNA analysis from teeth of a drowned body that existed in water for along term"DNA Polymorphism. 9. 132-134 (2001)

Hiroshi Ohira.：“利用线粒体 DNA 分析对长期存在于水中的溺水尸体的牙齿进行鉴定”DNA 多态性。