Extraction Method of Dental DNA by Using whole Tooth Sections : Application to Forensic Odontology

使用全牙切片提取牙齿 DNA 的方法:在法医牙医学中的应用

基本信息

  • 批准号:
    08672143
  • 负责人:
  • 金额:
    $ 0.9万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

I have previously reported the forensic value of teeth as a source of DNA for genetic analysis. Commonly, dental DNA has been obtained from dental pulp. The DNA thus obtained usually contains high-molecular-weight DNA and is suitable for multilocus DNA probe analysis. In most cases seen in forensic practice, sample teeth have been left in humid conditions and the dental pulp has been degraded by endonucleases, so that its condition is too poor for analysis. I have sometimes found that dental DNA obtained from a whole tooth section is badly degraded, and suitable only for polymerase chain reaction (PCR). Moreover, on several occasions, it is not amplified by the PCR technique even though it has been confirmed by agarose gel electrophoresis that there is enough DNA for the PCR.Therefore, I suspected that some cations contaminated in tooth sections might contaminate dental DNA during its extraction as a source material for PCR,and act as a inhibitor of Taq DNA polymerase during the PCR procedure.In this study, I demonstrated conclusively that the Chelex-based procedure tenders DNAs obtained from a whole tooth section suitable for PCR amplification, and the possibility of forensic DNA analysis using those of purified dental DNA.Dental identification is traditionally based on a comparison of antemortem with postmortem dental charts and X-rays. In cases where this is impossible the usefulness of the amplification of chromosal hypervariable loci and mitochondrial DNA sequencing are undisputed and it should use as an adjunct in forensic odontological practice.
我以前曾报道过牙齿作为遗传分析DNA来源的法医价值。通常,牙齿DNA已经从牙髓中获得。这样获得的DNA通常含有高分子量DNA,适合于多位点DNA探针分析。在法医实践中看到的大多数情况下,样本牙齿被放在潮湿的条件下,牙髓已被核酸内切酶降解,因此其状况太差,无法进行分析。我有时发现从整个牙齿切片中获得的牙齿DNA严重降解,只适合聚合酶链反应(PCR)。此外,有几次,即使琼脂糖凝胶电泳证实有足够的DNA用于PCR,PCR技术也不能扩增。因此,我怀疑牙齿切片中污染的某些阳离子可能在提取牙齿DNA作为PCR源材料时污染牙齿DNA,并在PCR过程中作为Taq DNA聚合酶的抑制剂。在本研究中,我证明了结论性的Chelex为基础的程序招标DNA从整个牙齿部分适合PCR扩增,和法医DNA分析的可能性,使用那些纯化的dental DNA.Dental识别传统上是基于比较生前与死后的牙科图表和X射线。在无法做到这一点的情况下,染色体高变基因座扩增和线粒体DNA测序的有用性是无可争议的,它应该在法医牙科实践中作为一种辅助手段。

项目成果

期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
鈴木廣一: "DNA多型Vol.4" 日本DNA多型学会, 294 (1996)
Hirokazu Suzuki:“DNA 多态性 Vol.4” 日本 DNA 多态性学会,294(1996)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yoshihiro Yamada: "Removal of some inhibitors in contaminated DNA from teeth by using a chelex 100 mini-column" ADVANCE IN LEGAL MEDICINE. 3. 196-199 (1997)
Yoshihiro Yamada:“使用 chelex 100 迷你柱去除牙齿中受污染 DNA 中的一些抑制剂”《法律医学进展》。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yoshihiro Yamada: "Removal of some inhibitors in contaminated DNA from teeth by using a chelex 100mini-column" ADVANCES IN REGAL MEDICINE. 3. 196-199 (1997)
Yoshihiro Yamada:“使用 chelex 100mini 柱去除牙齿中受污染 DNA 中的一些抑制剂”,皇家医学的进步。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yoshihiro Yamada: "Purification of high-quality dental DNA for PCR-based typing from whole tooth sections using a Chelex mini-column" Jpn.J.Oral Biol.39. 332-336 (1997)
Yoshihiro Yamada:“使用 Chelex 迷你柱从整个牙齿切片中纯化高质量的牙科 DNA,用于基于 PCR 的分型”Jpn.J.Oral Biol.39。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yoshihiro Yamada: "DNA Analysis in Forensic Practice 4-An Application oof Direct Sequencing for Forensic Practice" DNA POLYMORPHISM. 6 (in press). (1998)
Yoshihiro Yamada:“法医实践中的 DNA 分析 4 - 直接测序在法医实践中的应用”DNA 多态性。
  • DOI:
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  • 影响因子:
    0
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YAMADA Yoshihiro其他文献

YAMADA Yoshihiro的其他文献

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{{ truncateString('YAMADA Yoshihiro', 18)}}的其他基金

a Study on the changing role of Corporate Litigations in Corporate Governance
公司诉讼在公司治理中的角色转变研究
  • 批准号:
    17K03492
  • 财政年份:
    2017
  • 资助金额:
    $ 0.9万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A Theory-of-Mind Approach to Empathy-Related Phenomena
共情相关现象的心理理论方法
  • 批准号:
    20520345
  • 财政年份:
    2008
  • 资助金额:
    $ 0.9万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The Basic model for aquatic ecosystem assessment using carbon and nitrogen stable isotopes
碳氮稳定同位素水生生态系统评估基本模型
  • 批准号:
    18510023
  • 财政年份:
    2006
  • 资助金额:
    $ 0.9万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Regulation of tumor angiogenesis by transcription factor LMO2
转录因子 LMO2 对肿瘤血管生成的调节
  • 批准号:
    13670214
  • 财政年份:
    2001
  • 资助金额:
    $ 0.9万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Fundamental examination of repeat structure of the Y chromosome STR locus
Y染色体STR位点重复结构的基础检查
  • 批准号:
    13672177
  • 财政年份:
    2001
  • 资助金额:
    $ 0.9万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The Relation between Lexical Structures and Syntactic Structures
词汇结构和句法结构之间的关系
  • 批准号:
    10610449
  • 财政年份:
    1998
  • 资助金额:
    $ 0.9万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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