Analysis of bacterial biofilms as a virulence factor for peri-implantitis
细菌生物膜作为种植体周围炎毒力因子的分析
基本信息
- 批准号:13672184
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We investigated morphologically initial biofilm formation in the human implant sulcus using healing abutments. After obtaining informed consent from 7 patients, twenty healing abutments were removed carefully on day 10, 30, 90 and 150 after insertion and treated for a scanning electron microscopy. On day 10, mono-layer biofilm have partially formed on the abutment surfaces. So called early colonizers: cocci and filaments, were observed. After 30 days, biofilms developed, and long and short rods were predominantly observed in human implant sulcusNext, human periimplantitis associated bacteria have been examined immunohistochemically. Eight implants obtained from 4 voluntary patients were extracted and processed into semithin sections They were stained BrownBrenn modified Gram stain and streptavidinbiotin method using 4 kinds of antibodies with periodontopathic bactria. Gram positive bacteria were shown not only on the implant surface but also at the inner site of abutment screw, Porphyromonas gingivalis and Campylobacter rectus labeled with speciesspecific antibody were not found in the human implant sulcusBy use of the in vitro model with P. gingivalis biofilm, 1) the biological (ATP) activities, 2) the susceptibility to antimicrobial agents, and 3) the essential gene for development of biofilm (the homologue for polyphosphate kinase (ppk)) were examined. The rate of increase of ATP contents in the biofilm was quite low, and 1/3.0x10^5 compared with that of planktonic cells. Chlorhexidine gluconate was effective at reducing the viability of P. gingivalis biofilm, while minocycline hydrochloride and metronidazole did not have a variable effect. Identity of P. gingivalis ppk gene that was carried out cloning to Esherihia coli ppk gene was 35.8%, including \hat ppk from P. gingivalis was highly conserved
我们调查了形态学上的初始生物膜形成的人类种植沟愈合基台。在获得7名患者的知情同意后,在插入后的第10、30、90和150天小心地移除20个愈合基台,并进行扫描电子显微镜检查。在第10天,单层生物膜已部分形成在基牙表面上。观察到所谓的早期殖民者:球菌和丝状菌。30天后,生物膜形成,在人类种植体沟中主要观察到长杆和短杆。取4例患者的8枚种植体,取半薄切片,用4种抗牙周病菌抗体进行BrownBrenn改良革兰氏染色和链霉亲和素生物素法染色。在种植体表面和基台螺钉内均检测到革兰氏阳性菌,在人种植体龈沟中未检测到种属特异性抗体标记的牙龈卟啉单胞菌和直弯曲杆菌。(3)检测了生物膜形成所必需的基因(多磷酸激酶(ppk)的同源物)。生物膜中ATP含量的增加率很低,为正常细胞的1/3.0 × 10 ~ 5。葡萄糖酸氯己定可有效降低牙龈卟啉单胞菌生物膜的活力,而盐酸米诺环素和甲硝唑则无可变效应。所克隆的牙龈卟啉单胞菌ppk基因与大肠杆菌ppk基因的同源性为35.8%,其中牙龈卟啉单胞菌ppk基因高度保守
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Noiri Y., et al.: "Effects of chiorhexidine, minocycline and metronidazole on Porphyromonas gingivalis strain 381 in biofilm"Journal of Periodontology. 74(印刷中). (2003)
Noiri Y. 等人:“氯己定、米诺环素和甲硝唑对生物膜中牙龈卟啉单胞菌菌株 381 的影响”《牙周病学杂志》74(出版中)。
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
Noiri Y. et al.: "Effects of chlorhexidine, minocycline and metronidazole on Porphyromonas gingivalis strain 381 in bioflim"Journal of Periodontology. 74(印刷中). (2003)
Noiri Y. 等人:“氯己定、米诺环素和甲硝唑对生物膜中牙龈卟啉单胞菌菌株 381 的影响”《牙周病学杂志》74(出版中)。
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- 影响因子:0
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偲比須繁之(分担執筆): "バイオフィルム(Bacterial Biofilm):最近話題の用語 -知っておきたい豆知識 -"小児科. 44・4(増刊号)(印刷中). (2003)
Shigeyuki Amabisu(合著者):“细菌生物膜:最新术语 - 您应该知道的琐事 -”儿科学 44.4(特刊)(目前正在印刷)。
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- 影响因子:0
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Narimatsu,M: "Evaluation of baiological activity of periodontopathic bacterium Porphyromonas ging ivalis biofilm and Characterization of the biofilm-related genes"Bacterial adherence & Biofilm. 15. 8-13 (2001)
Narimatsu,M:“牙周病细菌牙龈卟啉单胞菌生物膜的生物学活性评估和生物膜相关基因的表征”细菌粘附
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成松雅博: "Porphyromonas gingivalisのputative glycosyltransferase geneのクローニングおよびバイオフィルム形成における役割解析"Bacterial Adherence & Biofilm. 16(印刷中). (2003)
Masahiro Narimatsu:“牙龈卟啉单胞菌的假定糖基转移酶基因的克隆及其在生物膜形成中的作用分析”《细菌粘附与生物膜》(2003 年)。
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NOIRI Yuichiro其他文献
NOIRI Yuichiro的其他文献
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{{ truncateString('NOIRI Yuichiro', 18)}}的其他基金
Establishment of micro-endodontic model in small animal and development of root canal treatment tschnique and root canal treatment drugs
小动物显微牙髓模型的建立及根管治疗技术及根管治疗药物的开发
- 批准号:
15H05021 - 财政年份:2015
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of experimental pathologic refractory periapical periodontitis model and investigation of its chemical supplemental methods
实验性病理性难治性根尖周炎模型的建立及其化学补充方法的探讨
- 批准号:
24390424 - 财政年份:2012
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of quorum sensing induced analogs in situ in human dental biofilms
人牙齿生物膜中群体感应诱导类似物的原位分析
- 批准号:
21390508 - 财政年份:2009
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of inhibitory agents against extraradicular biofilmassociated with chronic and refractory periapical periodontitis
开发针对与慢性和难治性根尖周炎相关的根外生物膜抑制剂
- 批准号:
18390505 - 财政年份:2006
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Basic study on suppression-effects of dental laser to bacterial biofilms
牙科激光对细菌生物膜抑制作用的基础研究
- 批准号:
15592019 - 财政年份:2003
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Studies on analysis of adhesion-colonization mechanism on Porphyromonas gingivalis in human periodontal pocket.
人牙周袋牙龈卟啉单胞菌粘附定植机制分析研究
- 批准号:
11672081 - 财政年份:1999
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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