Analysis of quorum sensing induced analogs in situ in human dental biofilms

本研究旨在建立一种对人牙生物膜进行化学控制和抑制的方法。N-酰基高丝氨酸内酯（阿勒）类似物是一种自诱导物，是群体感应（QS）诱导剂。10种阿勒类似物均未检出（6。32-7. 33 ng/ ml），说明人牙生物膜中几乎不存在阿勒。另一方面，我们发现了一些阿勒类似物和抗生素，表明抗生物膜作用会影响QS，并为这些化疗药物的化学生物膜控制方法的临床应用奠定了基础，我感到自豪的是，这些研究结果为细菌生物膜疾病的新治疗策略的发展提供了有意义的建议。

This study was carried out for the establishment of the chemical control and restraint method against human dental biofilm. N-Acyl homoserine lactone(AHL) analogs which was one kind of the autoinducer and was quorum sensing(QS) inducer were targeted. The 10 kinds of AHL analogs were not shown above detection limit(6. 32-7. 33 ng/ ml), and it was clarified that AHL hardly existed in human dental biofilm. On the other hand, We discovered some AHL analogs and antibiotics indicating the anti-biofilm action that would affect QS and made the basics for the clinical application of the chemical biofilm control method by these chemotherapeutic agents.I take pride in these results of the research giving a significant suggestion for the development of the new treatment strategy of the bacterial biofilm disease.