Basic study on suppression-effects of dental laser to bacterial biofilms

本文报道了Er：YAG激光对粪肠球菌、变形链球菌、粘性放线菌等4种单一菌种的影响。利用改进的Robbins装置研究了羟基磷灰石(HA)表面生物膜的形成。与对照组相比，所有受检组(20、40和80mJ辐射)变形链球菌和粘性葡萄球菌生物膜中的活细胞数均显著减少。20MJ组痤疮假单胞菌和粪肠球菌细胞数差异无统计学意义。3种不含痤疮假单胞菌的细菌生物膜形态发生改变，生物膜表面细胞扁平。在透明质酸纸片上制备牙龈卟啉单胞菌381生物膜。分别于灌流后0、1、3、7d用三磷酸腺苷(ATP)生物发光法观察其抗菌效果，并用扫描电子显微镜观察其形态。在c-…之间发现atp含量显著降低(p&lt；0.001)。氯己定治疗组和对照组。洗必泰处理后，细胞外基质结构和牙龈假单胞菌细胞结构发生改变。盐酸二甲胺四环素还可引起牙龈假单胞菌生物膜中三磷酸腺苷含量下降和形态改变。研究了根管内分离的粪肠球菌、血链球菌、中间链球菌、化脓性链球菌、金黄色葡萄球菌、核梭杆菌、痤疮假单胞菌、牙龈假单胞菌和中间普氏杆菌对牙胶点的初始生物被膜能力。粪肠球菌、血链球菌、中间链球菌、化脓性链球菌和金黄色葡萄球菌在添加45或90v%血清的培养液中孵育后，在标本表面形成单一菌种生物膜。粪肠球菌和血链球菌生物膜明显厚于中间链球菌、化脓性链球菌和金黄色葡萄球菌。与核杆菌、痤疮假单胞菌、牙龈假单胞菌和中间假单胞菌共同培养的标本均未检出生物膜。较少

The effects of Er:YAG laser on 4 single species (Enterococcus faecalis, Streptococcus mutans, Actinomyces viscosus. Propionibacterium acnes) biofilms formed on hydroxyapatite (HA) disks using modified Robbins Device was studied. The numbers of viable cells in S.mutans and A.viscosus biofilm were significantly reduced in all groups examined (20, 40, and 80 mJ radiation) compare to control groups. However, The number of P.acnes and E.faecalis cells were shown no significant differences in 20 mJ group. The biofilms of 3 bacterial species without P.acnes were morphologically changed, and the surface cells of the biofilms were flat.Porphyromonas gingivalis strain 381 biofilms were prepared on HA disks. At 0, 1, 3, and 7 days after perfusion of 3 antimicrobial agents, the antimicrobial effects by adenosine triphosphate (ATP) bioluminescence, and morphological investigation by scanning electron microscopy was performed. A significant decrease (p < 0.001) in ATP content was found between the c … More hlorhexidine-treated and control groups. The extracellular matrix structure and P.gingivalis cells were altered in the presence of chlorhexidine. Minocycline hydrochloride also caused a decrease in the ATP content and morphological change on P.gingivalis biofilms. Metronidazole showed no significant efficacy against P.gingivalis biofilms.The initial biofilm-forming ability of root canal isolates (E.faecalis, Streptococcus sanguis, Streptococcus intermedius, Streptococcus pyogenes, Staphylococcus aureus, Fusobacterium nucleatum, P.acnes, P.gingivalis and Prevotella intermedia) on gutta-percha points was investigated. Single species E.faecalis, S.sanguis, S.intermedius, S.pyogenes and S.aureus biofilms were generated on the surfaces of the specimens incubated in culture mediums supplemented with 45 or 90 v% serum. The E.faecalis and S.sanguis biofilms were significantly thicker than those of S.intermedius, S.pyogenes and S.aureus. No biofilms were detected on the specimens incubated with F.nucleatum, P.acnes, P.gingivalis, and P.intermedia. Less