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Studies on analysis of adhesion-colonization mechanism on Porphyromonas gingivalis in human periodontal pocket.

人牙周袋牙龈卟啉单胞菌粘附定植机制分析研究

基本信息

批准号：
11672081
负责人：
NOIRI Yuichiro
金额：
$ 2.56万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

We investigated whether Porphyromonas gingivalis fimbriae are formed in vivo, and the localization of P.gingivalis fimbriae in the human periodontal pockets. After obtaining informed consent from 27 patients. Six teeth with severe periodontitis with their surrounding periodontal tissue were extracted carefully and treated for a light microscopy. Some sections were stained immunohistochemically (LSAB method) used anti-fimbriae antibody. P.gingivalis labeled with anti-fimbriae antibody were found in the human periodontal pockets. In the shallow and middle pocket areas, P.gingivalis labeled with anti-fimbriae antibody were scattered from the tooth-attached to unattached plaque. In the deep pocket zones, it was observed at higher frequency close to the tooth surface than in the shallow and middle pocket zones. But P.gingivalis labeled with anti-fimbriae antibody werenot observed in the whole pocket-epithelium.Subgingival plaque bacteria live within a biofilm covered with glycocalyx, and little is known of P.gingivalis associated with biofilm formation at the buttom of human periodontal pockets, the so called "plaque-free zone" (PFZ). We examined P.gingivalis, which were forming biofilms in the PFZ by immunoelectron microscopic techniques, using both secondary and back-scattered imaging, with rabbit antibody. Positive reactions with anti-P.gingivalis were found in 8 of 13 samples examined, and film-like structures coated several cells of 6 positive samples examined. These findings demonstrated that P.gingivalis fimbriae play an important role in the process by which P.gingivalis initially adheres to tooth surface and may be related to the formation of biofilm st the bottom of periodontal pockets.

我们研究了牙龈卟啉单胞菌菌毛是否在体内形成，以及牙龈卟啉单胞菌菌毛在人牙周袋中的定位。在获得27名患者的知情同意后。对6颗重度牙周炎患牙及其周围牙周组织进行了仔细的拔除和光镜检查。用抗菌毛抗体对部分切片进行化学染色（LSAB法）。在人牙周袋中发现了标记有菌毛的牙龈卟啉单胞菌。在浅袋和中袋区，抗菌毛抗体标记的牙龈卟啉单胞菌从附着牙菌斑分散到未附着牙菌斑。在深槽区中，在靠近齿面处观察到的频率高于浅槽区和中槽区。龈下菌斑细菌生活在由糖萼覆盖的生物膜内，而牙龈卟啉单胞菌与人牙周袋底部的生物膜（即所谓的“无菌斑区”）的关系尚不清楚。我们检查牙龈卟啉单胞菌，这是形成生物膜的PFZ免疫电镜技术，使用二次和背散射成像，与兔抗体。13份标本中8份与抗牙龈卟啉单胞菌呈阳性反应，6份阳性标本中有多个细胞被膜样结构包裹。这些结果表明，牙龈卟啉单胞菌菌毛在牙龈卟啉单胞菌最初粘附于牙面的过程中起重要作用，并可能与牙周袋底部生物膜的形成有关。