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Co-localization of receptor and transducer proteins in glycolipid-enriched membrane domain.

受体和转导蛋白在富含糖脂的膜域中的共定位。

基本信息

批准号：
13680686
负责人：
KITAJIMA Ken
金额：
$ 2.5万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680686/
关键词：
LIPID RAFT MEMBRANE DOMAIN SEA URCHIN PIG EGG SPERM FERTILIZATION GLYCOLIPID

项目摘要

The objective of this research project was to demonstrate molecular mechanism for co-localization and recruitment of functionally cognate proteins into glycolipid-enriched microdomain. The results are following :1. Chemical and functional analyses of components of the glycolipid-enriched microdomain :(1) At least two distinct membrane microdomains, each of which contains functionally cognate molecules, are present in sea urchin sperm, based on analysis of immunoprecipitation experiments.(2) Comparison of components of sperm membrane microdomain from sea urchin with those from pig sperm shows that both commonly contain arylsulfatase, indicating the universal importance of sulfatide in fertilization.(3) Liposomes containing phospholipid, cholesterol and glycolipids that are reconstituted based on composition of the sperm membrane microdomain inhibit sea urchin fertilization.2. Elucidation of mechanism for recruitment of cognate components into glycolipid-enriched membrane microdomain :(1) The sea urchin sperm membrane microdomain has a high melting point and shows more rigid nature than whole membrane.(2) Recruitment of certain transducer proteins into the membrane microdomain dramatically changes during acrosome reaction after treatment of sperm with egg jelly components.(3) Much more membrane microdomain samples that are homogenous in components are required to elucidate molecular mechanism for the recruitment of components into the membrane microdomains physicochemically.

本研究项目的目的是展示功能同源蛋白在糖脂富集微域共定位和募集的分子机制。结果如下：1。糖脂富集微结构域成分的化学和功能分析：(1)根据免疫沉淀实验分析，海胆精子中存在至少两个不同的膜微结构域，每个膜微结构域都包含功能同源分子。(2)海胆和猪精子膜微结构域的成分比较表明，两者均含有芳基硫酸酯酶，表明硫酸酯在受精中的普遍重要性。(3)基于精子膜微结构域组成重组的含有磷脂、胆固醇和糖脂的脂质体抑制了海胆的受精。同源成分进入富含糖脂的膜微结构域的机制阐明：(1)海胆精子膜微结构域熔点高，比全膜刚性强。(2)卵胶成分处理精子后，顶体反应过程中某些换能器蛋白向膜微域的募集发生了显著变化。(3)需要更多组分均质的膜微域样品来阐明组分进入膜微域的分子物理化学机制。