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ANALYSIS OF STRIATAL LOCAL CIRCUITS BY SIMULTANEOUS PATCH CLAMP RECORDINGS FROM TWO CELLS

通过同时膜片钳记录两个细胞的纹状体局部回路进行分析

基本信息

批准号：
13680887
负责人：
AOSAKI Toshihiko
金额：
$ 1.92万
依托单位：
TOKYO METOROPOLITAN INSTITUTE OF GERONTOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

The basal ganglia is involved in adaptive control of action in the motor, planning and cognitive spheres (Graybiel, 1995). Corticostriatal relevant inputs, convergent or divergent, induce modular activation of neurons in patchy domains in the striatum, the input stage of the basal ganglia. These matrix domains called matrisomes consist of activated medium spiny (MS) projection neurons located in the larger modules determined by activated parvalbumin-positive g-aminobutyric acid (GABA)interneurons and NADPH diaphorase-positive GABA interneurons (Parthasarathy and Graybiel, 1997 ; Berretta, Parthasarathy and Graybiel, 1997). However, how cortical inputs are processed, integrated and output in the matrisomes still remains elusive. Using paired recordings in mouse corticostriatal slices, we found that the feed-forward inhibition of the MS cells by a network of parvalbumin interneurons predominates in the striatal local circuitry and that MS cells within a sphere of 〜150 μm in diameter are under the influence of a single parvalbumin interneuron. Moreover, the inhibition significantly improves the spike timing precision and the signal-to-noise ratio of the striatal outputs. Thus, parvalbumin cell networks in the striatum may take part in the emergence of functional cell assemblies that could influence the temporal order of striatal outputs.

基底神经节参与运动、计划和认知领域的适应性控制（Graybiel，1995）。皮质纹状体相关的输入，收敛或发散，诱导模块化激活的神经元在斑片状领域的纹状体，输入阶段的基底神经节。这些称为基质体的基质结构域由位于较大模块中的激活的中等多刺（MS）投射神经元组成，所述较大模块由激活的小清蛋白阳性g-氨基丁酸（GABA）中间神经元和NADPH黄递酶阳性GABA中间神经元确定（Parthasarathy和Graybiel，1997 ; Berretta，Parthasarathy和Graybiel，1997）。然而，皮层输入如何在matrisome中处理，整合和输出仍然是一个谜。利用小鼠皮质纹状体切片的成对记录，我们发现在纹状体局部回路中，小白蛋白中间神经元网络对MS细胞的前馈抑制占主导地位，直径为150 μm的球体内的MS细胞受到单个小白蛋白中间神经元的影响。此外，抑制显着提高了尖峰定时精度和信噪比的纹状体输出。因此，纹状体中的小白蛋白细胞网络可能参与功能细胞组装的出现，这些功能细胞组装可能影响纹状体输出的时间顺序。