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Roles of amygdalostriatal projection in the procedural learning

杏仁核纹状体投射在程序性学习中的作用

基本信息

批准号：
11480242
负责人：
AOSAKI Toshihiko
金额：
$ 5.18万
依托单位：
Tokyo Metropolitan Institute of Gerontology (TMIG)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11480242/
关键词：
striatum memory cholinergic learning procedural dopamine basal ganglia patch clamp アセチルコリン長期抑圧長期増強

项目摘要

The striatum, the input stage of the basal ganglia, is a critical brain structure for the learning of stimulus-response habits as well as motor, perceptual, and congnitive skills. Roles of dopamine (DA) and acetylcholine (ACh) in this form of implicit memory have long been considered essential, but the underlying cellular mechanism is still unclear. By means of patch clamp recordings from corticostriatal slices of the mouse, we studied whether the identified striatal cholinergic interneurons undergo long-term synaptic changes after tetanic stimulation of cortico and thalamostriatal fibers. Electrical stimulation of the fibers revealed a depolarizing and hyperpolarizing postsynaptic potential in the striatal cholineergic interneurons. The early depolarizing phase was considered as a cortico/thalamostriatal glutamatergic EPSP and the hyperpolarizing component as an intrastriatally-evoked GABAergic IPSP.Tetanic stimulation of cortico/thalamostriatal fibers was found to induce simultaneously occurring long-term potentiation (LTP) of the EPSPs as well as the disynaptically-mediated IPSPs. The induction of LTP of EPSP required a rise in intracellular Ca2+ concentration and dopamine D5, but not D2 receptor activation. Blockade of neither NMDA receptors, metabotropic glutamate receptors nor Ca2+ -permeable AMPA receptors had any significant effects. The long-term enhancement of the disynaptic IPSPs was due to a long-term increase in the occurrence rate, but not the amplitude of disynaptically-mediated IPSP in the striatal cholinergic interneurons. This dual mechanism of synaptic plasticity may be responsible for the long-term modulation of the cortico/thalamostriatal synaptic transmission.

纹状体是基底神经节的输入阶段，是学习刺激-反应习惯以及运动、感知和认知技能的关键大脑结构。多巴胺（DA）和乙酰胆碱（ACh）在这种形式的内隐记忆中的作用一直被认为是必不可少的，但其潜在的细胞机制仍不清楚。通过膜片钳记录的小鼠皮质纹状体切片，我们研究了是否确定纹状体胆碱能中间神经元进行强直刺激后的皮层和丘脑纹状体纤维的长时程突触的变化。电刺激的纤维揭示了去极化和超极化突触后电位在纹状体胆碱能中间神经元。早期去极化相被认为是皮层/丘脑-纹状体突触能的EPSP，超极化相被认为是纹状体内诱发的GABA能的IPSP，强直刺激皮层/丘脑-纹状体纤维可同时诱发EPSP和双突触介导的IPSP的长时程增强（LTP）。EPSP的LTP的诱导需要细胞内Ca ~（2+）浓度的升高和多巴胺D_5的增加，而不需要D_2受体的激活。阻断NMDA受体、代谢型谷氨酸受体和Ca 2+渗透性AMPA受体都没有任何显着影响。双突触IPSP的长期增强是由于出现率的长期增加，而不是纹状体胆碱能中间神经元中双突触介导的IPSP的幅度。这种突触可塑性的双重机制可能是皮层/丘脑-纹状体突触传递的长期调节机制。