Real-time analysis on activity-dependent glucose uptake by usiug brain slices

使用脑切片实时分析活动依赖性葡萄糖摄取

• 批准号: 13680893
• 负责人: YAMADA Katsuya
• 金额: $ 2.11万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680893/
• 关键词: glucose; fluorescence; uptake; brain; gloucose; flourescent; tracer

Energy supply in the brain solely depends on glucose. However, the relationship between brain activity and glucose supply has not been well understood. By using 2-NBDG as a fluorescent glucose tracer, we have for the first time visualized glucose uptake into a single, living mammalian cells (Yamada et al, J. Biol. Chem. 2000). Our aim in the present study was to visualize glucose uptake into neurons. In conclusion, neurons dissociated from ventromedial hypothalamus, substantia nigra pars reticulata, and visual cortex incorporated 2-NBDG and showed an increase in the fluorescence. 2-NBDG was applied to visual cortical slices while the underlying white matter was electrically stimulated. Changes in the fluorescence intensity were detected in the area above the stimulating electrodes. In vivo experiments were also conducted in the somatosensory barrel cortex while the whiskers were mechanically stimulated after injection of 2-NBDG into artery. 2-NBDG fluorescence was observed in the site where optical intrinsic signals were the highest, implying an activity-dependent glucose uptake although more detailed study should be conducted.

大脑的能量供应完全依赖于葡萄糖。然而，大脑活动和葡萄糖供应之间的关系尚未得到很好的理解。通过使用2-NBDG作为荧光葡萄糖示踪剂，我们第一次可视化葡萄糖摄取到单个活哺乳动物细胞中（Yamada等，J. Biol. Chem. 2000）。我们在本研究中的目的是可视化葡萄糖摄取到神经元。总之，神经元分离的腹内侧下丘脑，黑质网状部，视皮层纳入2-NBDG，并表现出增加的荧光。2-将NBDG应用于视觉皮层切片，同时电刺激下方的白色物质。在刺激电极上方的区域中检测荧光强度的变化。在体实验中，将2-NBDG注入动脉后，在机械刺激触须的同时，在躯体感觉皮层桶中进行。2-在光学固有信号最高的部位观察到NBDG荧光，这意味着活性依赖性葡萄糖摄取，但应进行更详细的研究。

项目成果

山田勝也: "エネルギー欠乏時に脳を守るATP感受性カリウムチャネルの働き"メディカルテクノロジー. 30・2. 165-166 (2002)

山田克也：“能量缺乏时保护大脑的 ATP 敏感钾通道的功能”医学技术 30・2（2002 年）。

Yamada, K., Inagaki,N.: "ATP-sensitive potassium channels in the Brain: a sensor of Iryposic conditions."News Physiol. Sci.. 17. 127-130 (2002)

Yamada, K.，Inagaki,N.：“大脑中 ATP 敏感的钾通道：红细胞状态的传感器。”新闻生理学。

山田勝也: "単一膵島細胞におけるグルコース取り込みと細胞内カルシウムの観察 分子糖尿病学の進歩-基礎から臨床まで-2001.監修 矢崎義雄 渥美他編"金原出版. 187 (2001)

Katsuya Yamada：“单个胰岛细胞中葡萄糖摄取和细胞内钙的观察。分子糖尿病科学的进展 - 从基础到临床实践 - 2001 年。由 Yoshio Yazaki 监督，Atsumi 等人 187（2001）。”

Yamada, K., et al.: "Protective role of ATP-sensitive potassium channels in hypoxia-induced generalized seizure"Science. 292. 1543-1546 (2001)

Yamada, K. 等人：“ATP 敏感钾通道在缺氧引起的全身性癫痫发作中的保护作用”《科学》。