Study of Electron Transfer in Biological Macromolecule by the Method of Labelled Electron with Muon

μ子标记电子法研究生物大分子中的电子传递

• 批准号: 14204039
• 负责人: NAGAMINE Kanetada
• 金额: $ 29.95万
• 依托单位: High Energy Accelerator Research Organization (2004)The High Energy Accelerator Research Organization (2002-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14204039/
• 关键词: protein; biological macromolecule; electron transfer in molecule; blood magnetism; brain research; muon spin relaxation; hemoglobin; beam channel; 生体分子内電子伝達; ミューエスアール; ラジオグラフィー; DNA; 生体高分子内電子伝達; スピン緩和; ミュエスアール法

By using the fundamental properties of muon, a spin-dependent radioactivity, the μSR method has been developed. The present research group has successfully applied this method to life science by the following two approaches.1) The method of labeled electron with muon which uses the phenomena of electron capture of positive muon during slowing-down process and detects the movement of the brought in electron by the μSR method.2) Probing blood magnetism by the sensitive magnetic probe of the μSR method and detecting the Oxy- and Deoxy- status of the blood to be applied for the functional biology e.g. brain research.For the development of these approaches for life science it is essential to take a simultaneous development of the experimental instrumentation related to the accelerator producing muon beam.Through the present research project, the following scientific achievements have been obtained for muon life science. 1) The first observation of protein electron transfer in water solution by the labeled electron method. 2) In order to explain a strange electron transport phenomena observed in DNA, some new idea was proposed. 3) For the first time, blood magnetism has been detected by the μSR method. A large application field will be opened along with the development of the muon beam instrumentation.At the same time, through the present project, the following instrumental achievements have been marked. 1) In order to conduct the pulsed μSR experiment at very high intensity pulsed muon source, the 126 channel segmented detection system has been constructed and successfully used for the above-mentioned experiments. 2) The upgrading program has been carried out for the Dai-Omega, Large-Solid-Angle Axial-Focussing Superconducting Surface Muon Channel to produce the world-strongest pulsed muon beam.

利用μ子的基本性质，发展了μSR方法。本课题组通过以下两种途径成功地将该方法应用于生命科学：1）μ子标记电子法，利用正μ子慢化过程中的电子俘获现象，用μSR方法检测带入电子的运动; 2）用μ SR方法的灵敏磁探针探测血液磁性，检测血液中的氧和脱氧-血液在功能生物学（如脑研究）中的应用现状。为了发展生命科学的这些方法，必须同时发展与产生μ子束的加速器相关的实验仪器。通过本研究项目，在μ子生命科学方面取得了以下科学成果。1)用标记电子法首次观察到蛋白质在水溶液中的电子转移。2)为了解释在DNA中观察到的一种奇怪的电子输运现象，提出了一些新的观点。3)首次用μSR方法检测了血液磁性。随着μ子束测量仪器的发展，将沿着开辟一个广阔的应用领域。1)为了在极高强度脉冲μ子源上进行脉冲μSR实验，构建了126通道分段探测系统并成功用于上述实验。2)升级计划已经为Dai-Omega，大立体角轴向聚焦超导表面μ子通道进行，以产生世界上最强的脉冲μ子束。

项目成果

Introductory Muon Science

• DOI: 10.1017/cbo9780511470776
• 发表时间: 2003-10
• 作者: K. Nagamine

High Intensity Surface Muon Beam Using a Large Acceptance Axial Focussing Channel

使用大接收轴向聚焦通道的高强度表面 μ 子束

• 发表时间: 2004
• 期刊: AIP Conference Proceedings 721
• 作者: H.Miyadera;K.Nagamine;et al.
• 通讯作者: et al.

Advanced muon radiography with compact accelerator system

• DOI: 10.2183/pjab.80.179
• 发表时间: 2004-03
• 期刊: Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
• 作者: K. Nagamine

Inter-chain protein electron transfer for cytochrome c in water solution revealed by the muon labeling method

μ子标记法揭示水溶液中细胞色素c的链间蛋白质电子转移

• 发表时间: 2005
• 期刊: J.Phys.Condensed Matter (In print)
• 作者: Y.Ikedo;K.Nagamine;I.Igarashi;K.Shimomura et al.
• 通讯作者: K.Shimomura et al.

Advanced muon radiography with compact accelerator syste

具有紧凑型加速器系统的先进μ子射线照相

• 发表时间: 2005
• 期刊: Proc.Jpn.Acad., Sci. B80
• 作者: H.Tanaka;K.Nagamine;H.Miyadera;K.Shimomura;Y.Ikedo;K.Nishiyama;K.Ishida;K.Nagamine
• 通讯作者: K.Nagamine