Biological Macromolecule

生物高分子

• 批准号: 8559564
• 负责人: GORDON D GINDER
• 金额: $ 3.76万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8559564
• 关键词: Accounting; Address; Affinity Chromatography; Alleles; Area; Attention; Baculoviruses; Biochemistry; Biological; Cancer Center; Cancer Center Support Grant; Cancer Control; Cell Culture Techniques; Cell Line; Cloning; Collaborations; Communities; Complementary DNA; Complex; Consult; Consultations; Custom; DNA; DNA Insertion Elements; DNA Restriction Enzymes; DNA Sequence; Dependovirus; Detection; Drug resistance; Ensure; Flow Cytometry Shared Resource; Funding; Gene Proteins; Generations; Genes; Genetic Recombination; Genome; Genomics; Goals; Human; Individual; Insecta; Knock-in Mouse; Knock-out; Knockout Mice; Laboratories; Ligation; Malignant Neoplasms; Mammalian Cell; Maps; Methods; Molecular; Molecular Biology; Mus; Nature; Neomycin; Peer Review; Plasmids; Procedures; Process; Production; Protein p53; Proteins; Reaction; Recombinant Proteins; Recombinants; Research; Research Personnel; Resistance; Resource Sharing; Resources; Scheme; Services; Site; Somatic Cell; Specialist; Staging; Structure; System; Technology; Time; Training; Transgenic Organisms; Tumor Cell Line; Tumor Suppressor Genes; Universities; Validation; Vertebral column; Virginia; Virion; Virus; Yeasts; base; design; design and construction; embryonic stem cell; experience; expression vector; homologous recombination; instrumentation; interest; macromolecule; meetings; member; protein expression; screening; structural biology; tool; vector

The Biological Macromolecule Shared Resource (BMSR) represents a consolidation of services previously offered by the Molecular Biology and the Virus Vector shared resources, with the addition of a new service for recombinant protein production and purification. The overall goal of the BMSR is to facilitate and implement multiple end-points, both those within this shared resource, and those involving interfacing with other shared resources. These include the production of research grade virus particles, knock-in/knock-out mice (in collaboration with the Transgenic/Knockout Mouse Shared Resource), knock-in/knock-out somatic cell lines using homologous recombination, transient and stable protein expression in mammalian cells, recombinant protein production (bacterial, yeast, insect, and mammalian systems) and purification for structural analysis (in collaboration with the Structural Biology Shared Resource), and for protein interaction studies using the Biacore instrumentation located in the Flow Cytometry shared resource. In addition to moving vectors provided by investigators towards these end points, we offer design, construction and validation services for the generation of new vectors. The BMSR is co-directed by Dr Shirley Taylor (CMG), who brings molecular biology, cell culture, and protein expression experience, and Dr Darrell Peterson (Department of Biochemistry and Molecular Biology) a protein biochemist with extensive experience in protein production and purification. The BMSR employs a Resource Manager (Ms Kimberly Stratton, MS) and two full time research specialists, each of whom takes primary responsibility for one of the three major areas, but with sufficient cross-training in ail services offered to ensure continuity and maximal efficiency. The BMSR generally operates at >85% capacity, serving a wide range of investigators both within the Virginia Commonwealth University (VCU) Massey Cancer Center (MCC), and from the VCU research community. For the period January 1 2008 through December 31, 2010, MCC members with peer-reviewed funding accounted for approximately 70% of basic services and special projects. In CY 2010 the virus vector services of the BMSR served five funded MCC investigators, and three non-MCC investigators. Since its inception in January 2010, the Protein Production facility has served five funded MCC investigators and two non-MCC investigators, on ten separate projects. The BMSR served 22 peer-reviewed funded MCC investigators in routine services and special projects and 15 non-MCC investigators.

生物大分子共享资源（BMSR）整合了先前分子生物学和病毒载体共享资源提供的服务，并增加了重组蛋白生产和纯化的新服务。BMSR的总体目标是促进和实现多个端点，这些端点既包括共享资源中的端点，也包括涉及与其他共享资源接口的端点。这些包括生产研究级病毒颗粒、敲入/敲除小鼠（与转基因/敲除小鼠共享资源合作）、使用同源重组的敲入/敲除体细胞系、哺乳动物细胞中的瞬时和稳定蛋白质表达、重组蛋白质生产（细菌、酵母、昆虫和哺乳动物系统）和纯化用于结构分析（与结构生物学共享资源合作）。以及使用位于流式细胞术共享资源中的Biacore仪器进行蛋白质相互作用研究。除了将研究人员提供的载体移动到这些终点之外，我们还为新载体的生成提供设计、构建和验证服务。BMSR由Shirley Taylor博士（CMG）和Darrell Peterson博士（生物化学和分子生物学系）共同领导，前者具有分子生物学、细胞培养和蛋白质表达经验，后者是蛋白质生物化学家，在蛋白质生产和纯化方面具有丰富经验。BMSR聘请了一名资源经理（Kimberly Stratton女士，硕士）和两名全职研究专家，每人主要负责三个主要领域之一，但在所有提供的服务方面都进行了充分的交叉培训，以确保连续性和最高效率。