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Puffer Culture Using Puffer Toxin-Immunopotentiation and Disease Prevention

河豚毒素培养-免疫增强和疾病预防

基本信息

批准号：
14206025
负责人：
ARAKAWA Osamu
金额：
$ 34.11万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Assuming the practical use of "puffer culture using puffer toxin (tetrodotoxin ; TTX)", the tiger puffer Takifugu rubripes (larvae, 1-year-, or 2-years-old fish) were reared with several. types of TTX-containing diets in an actual culture field or in the similar conditions, and investigated for their toxin accumulation, health conditions, immunofunctions, etc. As the results, potentiation of both antibody productivity and splenocyte proliferation reaction were seen in the test fish administered with TTX irrespective of their age, indicating that the feeding of TTX-containing diets could be effective for growing up healthy puffers also in practical scales. When the puffers were administered with paralytic shellfish poison (PSP), they could accumulate it less efficiently than TTX, where no immunopotentiating effect was recognized.Two types of TTX, purified and crude, were injected intraperitoneally into T.rubripes. Both the toxins administered moved mainly into skin and liver, though the … More purified one seemed to move more rapidly, while the crude one was accumulated much more densely. In addition, change in splenocyte proliferation reaction correlating with the movement of TTX was observed.Micro-distribution of TTX in the tissues of TTX-bearing organisms was visualized by an immuno-enzymatic technique. In pufferfishes, TTX was distributed mainly in basal cells and succiform cells of the skin, yolk vesicles of oocytes, lysosomes of the basal cells, etc., although the distribution pattern varies depending on the species. In case of the ribbon worm Cephalothrix sp., TTX was recognized in the vesicles apically arranged in the bacillary cells, the granular cells in the proboscis, rhynchocoel epithelium, etc., whereas in the flatworm Planocera reticulata, only in the ovum.In addition to the above results, it could also be clarified that the marine puffer Arothron firmamentum had a considerable amount of PSP in the matured ovary, and that the T.rubripes cultured with non-toxic diet was completely non-toxic. Less

假定“使用河鲀毒素（河鲀毒素；TTX）的河鲀养殖”的实用化，饲养了数条虎鲀红鳍东方鲀（幼鱼、1年生、2年生鱼）。在实际养殖场或类似条件下，对含TTX的饲料类型进行研究，并调查其毒素积累、健康状况、免疫功能等。结果，无论年龄如何，在施用TTX的试验鱼中都观察到抗体生产力和脾细胞增殖反应的增强，这表明饲喂含TTX的饲料对于健康河豚的成长也有效。秤。当河鲀注射麻痹性贝毒（PSP）时，它们的积累效率低于TTX，而TTX没有发现免疫增强作用。将两种类型的TTX（纯化的和粗制的）腹膜内注射到红鳍鲀体内。所施用的两种毒素主要进入皮肤和肝脏，尽管纯化的毒素似乎移动得更快，而粗制的毒素则积累得更密集。此外，观察到与TTX运动相关的脾细胞增殖反应的变化。通过免疫酶技术使TTX在携带TTX的生物体的组织中的微观分布可视化。在河豚中，TTX主要分布在皮肤的基底细胞和琥珀状细胞、卵母细胞的卵黄囊、基底细胞的溶酶体等中，但分布模式因种类而异。对于带状蠕虫 Cephalothrix sp.，TTX 在杆菌细胞顶部排列的囊泡、喙中的颗粒细胞、喙腔上皮等中被识别，而在扁虫 Planocera reticulata 中，仅在卵子中被识别。除了上述结果之外，还可以澄清海洋河豚 Arothron fimamentum 中存在相当多的量。成熟卵巢中PSP的含量，并且用无毒饲料培养的红鳍鱼完全无毒。较少的