Puffer Culture Using Puffer Toxin-Immunopotentiation and Disease Prevention

假设实际使用“河豚毒素(河豚毒素；河豚毒素)养殖河豚”，用几条河豚(幼体、1岁或2岁的鱼)饲养虎河豚。在实际养殖场或相似条件下，对不同类型的河豚饲料进行了毒素积累、健康状况、免疫功能等方面的研究。结果表明，无论年龄大小，投喂河豚毒素的试验鱼的抗体生成和脾细胞增殖反应均有增强作用，说明投喂含河豚毒素饲料对健康河豚的饲养也是有效的。当给予河豚麻痹性贝毒(PSP)时，它们积聚PSP的效率低于TTX，后者没有免疫增强作用。两种类型的TTX，纯化的和粗制的，被注射到红色毛滴虫体内。这两种毒素都主要进入皮肤和肝脏，尽管…更纯净的似乎移动得更快，而粗制的似乎积聚得更密集。此外，还观察了与TTX运动相关的脾细胞增殖反应的变化。用免疫酶技术观察了TTX在带TTX生物体内的微观分布。在河豚中，TTX主要分布在皮肤的基细胞和乳汁状细胞、卵母细胞的卵黄小泡、基细胞的溶酶体等，但分布模式因物种而异。在头孢菌丝虫中，TTX存在于杆状细胞顶端排列的小泡、鼻部颗粒细胞、绒毛膜上皮等处，而在网纹扁平线虫中仅存在于卵子中。除上述结果外，还可以明确海豚成熟卵巢中含有相当数量的PSP，而用无毒饲料培养的红色丝虫则完全无毒。较少

Assuming the practical use of "puffer culture using puffer toxin (tetrodotoxin ; TTX)", the tiger puffer Takifugu rubripes (larvae, 1-year-, or 2-years-old fish) were reared with several. types of TTX-containing diets in an actual culture field or in the similar conditions, and investigated for their toxin accumulation, health conditions, immunofunctions, etc. As the results, potentiation of both antibody productivity and splenocyte proliferation reaction were seen in the test fish administered with TTX irrespective of their age, indicating that the feeding of TTX-containing diets could be effective for growing up healthy puffers also in practical scales. When the puffers were administered with paralytic shellfish poison (PSP), they could accumulate it less efficiently than TTX, where no immunopotentiating effect was recognized.Two types of TTX, purified and crude, were injected intraperitoneally into T.rubripes. Both the toxins administered moved mainly into skin and liver, though the … More purified one seemed to move more rapidly, while the crude one was accumulated much more densely. In addition, change in splenocyte proliferation reaction correlating with the movement of TTX was observed.Micro-distribution of TTX in the tissues of TTX-bearing organisms was visualized by an immuno-enzymatic technique. In pufferfishes, TTX was distributed mainly in basal cells and succiform cells of the skin, yolk vesicles of oocytes, lysosomes of the basal cells, etc., although the distribution pattern varies depending on the species. In case of the ribbon worm Cephalothrix sp., TTX was recognized in the vesicles apically arranged in the bacillary cells, the granular cells in the proboscis, rhynchocoel epithelium, etc., whereas in the flatworm Planocera reticulata, only in the ovum.In addition to the above results, it could also be clarified that the marine puffer Arothron firmamentum had a considerable amount of PSP in the matured ovary, and that the T.rubripes cultured with non-toxic diet was completely non-toxic. Less