Puffer Culture Using Puffer Toxin-Immunopotentiation and Disease Prevention

假设实际使用的“河豚养殖使用河豚毒素（河豚毒素; TTX）"，虎河豚红鳍东方鲀（幼虫，1岁，或2岁的鱼）饲养与几个。在实际养殖场或类似条件下，对含TTX的饲料类型进行了研究，并调查了它们的毒素积累、健康状况、免疫功能等。结果，在施用TTX的试验鱼中，无论其年龄如何，都观察到抗体生产力和脾细胞增殖反应的增强，表明在实际规模中，饲喂含河豚毒素的饲料也可有效地培育健康的河豚。当河豚与麻痹性贝类毒素（PSP）管理，他们可以积累它的效率低于TTX，没有免疫增强作用是recognized.Two类型的TTX，纯化和粗，腹腔注射到T. rubripes。这两种毒素都主要进入皮肤和肝脏， 关于我们 纯净的似乎移动得更快，而粗糙的则堆积得更密集。此外，还观察了与TTX运动相关的脾细胞增殖反应的变化，并用免疫酶技术观察了TTX在携带TTX生物体组织中的微分布。河豚鱼的TTX主要分布在皮肤的基底细胞和腺样细胞、卵母细胞的卵黄囊、基底细胞的溶酶体等，尽管分布模式因物种而异。在带状蠕虫头丝菌属的情况下，TTX在杆状细胞、吻部颗粒细胞、喙腔上皮等的顶端排列的小泡中被识别，而扁虫Planocera reticulata仅存在于卵子中。除了上述结果外，还可以明确，海洋河豚Arothron firmamentum成熟卵巢中含有相当数量的PSP，而用无毒饲料培养的红鳍叉尾鲀完全无毒。少

Assuming the practical use of "puffer culture using puffer toxin (tetrodotoxin ; TTX)", the tiger puffer Takifugu rubripes (larvae, 1-year-, or 2-years-old fish) were reared with several. types of TTX-containing diets in an actual culture field or in the similar conditions, and investigated for their toxin accumulation, health conditions, immunofunctions, etc. As the results, potentiation of both antibody productivity and splenocyte proliferation reaction were seen in the test fish administered with TTX irrespective of their age, indicating that the feeding of TTX-containing diets could be effective for growing up healthy puffers also in practical scales. When the puffers were administered with paralytic shellfish poison (PSP), they could accumulate it less efficiently than TTX, where no immunopotentiating effect was recognized.Two types of TTX, purified and crude, were injected intraperitoneally into T.rubripes. Both the toxins administered moved mainly into skin and liver, though the … More purified one seemed to move more rapidly, while the crude one was accumulated much more densely. In addition, change in splenocyte proliferation reaction correlating with the movement of TTX was observed.Micro-distribution of TTX in the tissues of TTX-bearing organisms was visualized by an immuno-enzymatic technique. In pufferfishes, TTX was distributed mainly in basal cells and succiform cells of the skin, yolk vesicles of oocytes, lysosomes of the basal cells, etc., although the distribution pattern varies depending on the species. In case of the ribbon worm Cephalothrix sp., TTX was recognized in the vesicles apically arranged in the bacillary cells, the granular cells in the proboscis, rhynchocoel epithelium, etc., whereas in the flatworm Planocera reticulata, only in the ovum.In addition to the above results, it could also be clarified that the marine puffer Arothron firmamentum had a considerable amount of PSP in the matured ovary, and that the T.rubripes cultured with non-toxic diet was completely non-toxic. Less