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Elucidation of command mechanism that bears the function of high performanced sink of bamboo

承担高性能竹水槽功能的指挥机构的阐述

基本信息

批准号：
14360098
负责人：
AZUMA Jun-ichi
金额：
$ 9.6万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

The aim of this study is to elucidate the command mechanism that bears the function of high performance sink of bamboo. We adopted rice microarray system because of the similarity of genes between bamboo and rice.We extracted mRNA from bamboo shoot, immature 17th culm (both upper and lower parts) and mature leaves, and synthesized cDNA for microarray analysis. Microarray analysis revealed that the specific genes to bamboo shoot are 78, and to immature lower part of culm are 635. In bamboo shoot, specific genes are mostly involved in DNA replication, protein synthesis. This indicates that cell division are very active in bamboo shoot. Also, gibberellin related genes are highly expressed, and this results support that gibberellin is involved in internode elongation from the object of molecular level.In lower part of culm, we selected strongly expressed 34 genes. These genes includes many sugar metabolism related genes, cell-cell communication and water transport (aqauaporin). Especially, sucrose synthase genes (Sus) were highly expressed in lower part of culm. We have cloned 3 Sus genes from bamboo. Two of them belong to Sus1 type group, and one belongs to SusA type group. RT-PCR and Western blot hybridization were carried out to analyze Sus genes expression. In young tissue (shoot and lower culm), Sus1 type gene expressed and as tissue matures expression level of Sus1 gradually decreased and of SusA type gene increased. Our results indicates that Suc genes play an important role in bamboo shoot elongation as sink function.

本研究旨在阐明竹子高性能贮库功能的调控机制。由于竹子和水稻基因组的相似性，我们采用水稻基因芯片技术，从竹笋、未成熟的第17秆（上下两部分）和成熟叶片中提取mRNA，合成cDNA用于基因芯片分析。基因芯片分析表明，竹笋特异基因有78个，竹下段特异基因有635个。在竹笋中，特异基因大多参与DNA复制、蛋白质合成。这说明竹笋的细胞分裂非常活跃。同时，赤霉素相关基因也得到了高表达，这一结果从分子水平上支持了赤霉素参与节间伸长的目的。这些基因包括许多与糖代谢相关的基因、细胞间通讯和水转运相关的基因（aqauaporin）。蔗糖合成酶基因（Sus）在茎下部表达量较高。我们从竹子中克隆了3个Sus基因。其中2个属Sus 1型群，1个属SusA型群。采用RT-PCR和Western blot杂交方法分析Sus基因的表达。Sus 1型基因在幼嫩组织（茎和下部茎）中表达，随着组织成熟，Sus 1型基因表达量逐渐降低，SusA型基因表达量逐渐增加。这些结果表明Suc基因在竹笋伸长过程中起着重要的库功能。