Region specific morphogenesis of the axial skeleton in avian embryos: tissue interactions and molecular mechanism

禽类胚胎中轴骨骼的区域特异性形态发生：组织相互作用和分子机制

• 批准号: 14370006
• 负责人: AOYAMA Hirohiko
• 金额: $ 8.83万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14370006/
• 关键词: bodyplan; morphogenesis; vertebrate; avian embryo; somitic mesoderm; axial skeleton; body wall muscles; phylogenesis; 個体発生; 傾倒発生; ニワトリ-ウズラキメラ; 遺伝子発現パターン; ニワトリ-ウズラ キメラ; 肋骨; 標識追跡; 助骨

Vertebrates are considered to be constructed by repeating and modifying segmental unit. Fundamentally, its developmental unit is somite. Not only the skeleton and muscles derived from somites, but also development of the spinal nerve and sympathetic trunk is under control of somites. To consider how the body plan is established in the vertebrate, we have investigated the mechanism of development from somites into the axial skeleton. Especially, our interest is focused on the development of the rib that develops region-specifically in the avian embryo.1. Three developmental compartment of the rib : Examination of interaction between the somites and their surrounding tissues showed that small proximal part of the rib articulating with the vertebra (proximal rib) develops depending on the notochord and the floor plate of neural tube, and long compartment other than the proximal rib (distal rib) develops depending on the surface ectoderm. Further, the distal rib can be divided into tow par … More ts. Somite cells migrate into the somatopleura form a part of the rib distal to the intra-rib articulation (sternal distal rib), while somite cells which do not enter the somatopleura form a part proximal to this joint (vertebral distal rib). These belong to the abaxial and primaxial compartment, respectively, which are recently proposed by Ann Burke.2. Distal rib morphogenesis and somite differentiation : When we inserted thin film between the somite and the surface ectoderm to block the interaction between them, lateral part of the dermomyotome expressing Sim1 and sclerotomal cells at the periphery of dermomyotome expressing Scleroaxis were missing. These regions are suggested to support the distal rib development.3. Region specific morphogenesis of trunk body wall muscles : Segmental unit of abdominal muscles.(1) Fate mapping: We showed that most part of the abdominal muscles was derived from the 27th somite and the 26th and the 28th somite formed the rostral and the caudal end region, respectively. Other lumbar somites form either the ribs or the trunk body wall muscles.(2) Innervations: In adult hen and chicken just after birth, we found that the abdominal muscles were largely innervated by the thoracic nerves. Because the somitic origin of abdominal muscles and the spinal nerve innervating them were from different levels along rostro-caudal axis, innervations will not always allow us to know the developmental origin of muscles.4. Limb development and rib development : When we induced a limb at the flank region in a chick embryo, the sternal distal ribs were missing. Both the limb and the sternal rib belong to the abaxial compartment and the embryo might allow to develop only one of them.5. Determination of the primordium of the axial skeleton : We transplanted heterotopically the quail epidermis or primitive streak into chick embryo and examined expression of Hox genes and morphology of resultant skeleton, showing that a part of epiblast where the somitic mesoderm arises was determined to become a certain type of vertebrae and/or ribs just before gastrulation. Less

脊椎动物被认为是通过重复和修改节段单元而构建的。从根本上来说，它的发育单位是体节。不仅骨骼和肌肉来源于体节，脊神经和交感干的发育也受体节的控制。为了考虑脊椎动物的身体规划是如何建立的，我们研究了从体节到中轴骨骼的发育机制。特别是，我们的兴趣集中在禽类胚胎中特定区域发育的肋骨的发育上。1。肋骨的三个发育区室：对体节与其周围组织之间相互作用的检查表明，与椎骨相连的肋骨近端小部分（近侧肋骨）依赖于脊索和神经管底板发育，近侧肋骨以外的长区室（远侧肋骨）依赖于表面外胚层发育。此外，远端肋骨可分为两部分。体节细胞迁移到体胸膜，形成肋骨内关节远端肋骨的一部分（胸骨远端肋骨），而不进入体胸膜的体细胞形成该关节近端的部分（椎骨远端肋骨）。它们分别属于远轴室和原轴室，这是 Ann Burke 最近提出的。2。远端肋骨形态发生和体节分化：当我们在体节和表面外胚层之间插入薄膜以阻止它们之间的相互作用时，表达 Sim1 的 dermomyotome 的外侧部分和表达 Scleroaxis 的 dermomyotome 外围的巩膜细胞缺失。建议这些区域支持远端肋骨的发育。3。躯干体壁肌肉的区域特异性形态发生：腹部肌肉的节段单位。（1）命运图谱：我们发现腹部肌肉的大部分源自第27体节，第26和第28体节分别形成头端和尾端区域。其他腰部体节形成肋骨或躯干体壁肌肉。(2)神经支配：在刚出生的成年母鸡和小鸡中，我们发现腹部肌肉主要受胸神经支配。由于腹部肌肉的体节起源和支配它们的脊神经沿着头尾轴来自不同的水平，神经支配并不总是能让我们知道肌肉的发育起源。 4．肢体发育和肋骨发育：当我们在鸡胚的侧翼区域诱导肢体时，胸骨远端肋骨缺失。肢体和胸骨肋骨均属于远轴室，胚胎可能只允许发育其中之一。5．中轴骨骼原基的确定：我们将鹌鹑表皮或原条异位移植到鸡胚中，并检查了Hox基因的表达和所得骨骼的形态，结果表明，在原肠胚形成之前，出现体节中胚层的外胚层部分被确定为某种类型的椎骨和/或肋骨。较少的

项目成果

Three developmental compartments involved in rib formation

• DOI: 10.1387/ijdb.041932ha
• 发表时间: 2005-01-01
• 期刊: INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
• 影响因子: 0.7
• 作者: Aoyama, H;Mizutani-Koseki, Y;Koseki, H
• 通讯作者: Koseki, H

Semite development without influence of the surface ectoderm in the chick embryo : The compartments of a somite responsible for distal rib development

不受鸡胚表面外胚层影响的闪族发育：负责远端肋骨发育的体节区室

• 发表时间: 2004
• 期刊: Development, Growth and Differentiation 46
• 作者: Hirao;A.;Aoyama;H.
• 通讯作者: H.

Three developmental compartments of the rib : the proximal rib develops depending on the ventro-axial tissues and the distal rib develops depending on the surface ectoderm including the sternal rib that depends on the lateral plate mesoderm.

肋骨的三个发育区室：近端肋骨的发育取决于腹轴组织，远端肋骨的发育取决于表面外胚层，包括取决于侧板中胚层的胸骨肋骨。

• 发表时间: 2005
• 期刊: International Journal of Developmental Biology (印刷中)
• 作者: Aoyama;H.;Mizutani-Koseki;Y.;Koseki;H.
• 通讯作者: H.

Somite development without influence of the surface ectoderm in the chick embryo : The compartments of a somite responsible for distal rib development.

不受鸡胚表面外胚层影响的体节发育：负责远端肋骨发育的体节区室。

• 发表时间: 2004
• 期刊: Development Growth Differentiation 46
• 作者: Hirao;A.;Aoyama;H.
• 通讯作者: H.