Metabolic regulation and intracellular transport of phospholipids in mammalian cells
哺乳动物细胞中磷脂的代谢调节和细胞内转运
基本信息
- 批准号:14380341
- 负责人:
- 金额:$ 9.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1)Study on the metabolic regulation of phospholipidsPhosphatidylserine (PS) in mammalian cells is synthesized through the action of ER membrane enzymes, PS synthase (PSS) 1 and 2, activities of which are post-translationally regulated by PS-mediated inhibition.(1)We purified Chinese hamster PSS 2 to near homogeneity. The kinetic analysis of the purified enzyme suggested that PSS 1 was inhibited by the binding of PS to a putative regulatory site of the enzyme.(2)To identify amino acid residues crucial for activity and/or regulation of PSS 1, we systematically introduced alanine replacement mutations into a Chinese hamster PSS 1 cDNA clone. On analysis of CHO cells transfected with each of the mutant clones, we identified 8 and 6 amino acid residues, respectively, as those crucial for the enzyme activity and regulation.(3)Membrane topology analysis of PSS 1 suggested that PSS 1 contained ten transmembrane segments, which placed the N and C termini in the cytosol. Furthermore, it was suggested that all of the eight amino acid residues important for the activity were placed from the center to luminal side of ER membrane, and that all of six amino acid residues important for enzyme regulation were exposed to the cytosol.2)Study on the intracellular transport of phospholipids(1)We identified a novel mammalian protein, MSBP1 involved in the PS transport from ER to mitochondria.(2)We isolated 〜40 candidates for yeast mutants that were defective in the intracellular transport of PS.
1)磷脂代谢调控的研究哺乳动物细胞中磷脂酰丝氨酸(PS)是通过内质网膜酶PS合成酶(PSS) 1和2的作用合成的,其活性通过PS介导的抑制作用在翻译后调控。(1)对中国仓鼠pss2进行纯化,达到接近同源性。纯化酶的动力学分析表明,PS与PSS 1的一个假定的调控位点结合可以抑制PSS 1。(2)为了鉴定对PSS 1活性和/或调控至关重要的氨基酸残基,我们系统地将丙氨酸替代突变引入中国仓鼠PSS 1 cDNA克隆中。通过对转染每个突变克隆的CHO细胞的分析,我们分别鉴定出8个和6个氨基酸残基,这些氨基酸残基对酶的活性和调控至关重要。(3) PSS 1的膜拓扑分析表明,PSS 1包含10个跨膜片段,这些跨膜片段将N和C末端置于细胞质中。此外,研究表明,所有8个对酶活性重要的氨基酸残基都被放置在内质网膜的中心到腔侧,所有6个对酶调节重要的氨基酸残基都暴露在细胞质溶胶中。(1)我们发现了一种新的哺乳动物蛋白MSBP1,它参与了磷脂从内质网到线粒体的转运。(2)我们分离了约40个存在PS细胞内转运缺陷的酵母突变体。
项目成果
期刊论文数量(18)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Functional analysis of Chinese hamster phosphatidylserine synthase 1 through synstematic alanine mutagenesis
通过系统丙氨酸诱变对中国仓鼠磷脂酰丝氨酸合酶1进行功能分析
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Ohsawa;T.;Nishijima;M.;Kuge;O.
- 通讯作者:O.
Requirement of an IkappaB-beta COOH terminal region protein for acidic-adaptation in CHO cells
CHO 细胞中酸性适应需要 IkappaB-beta COOH 末端区域蛋白
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Kuge;O;Qizong Lao
- 通讯作者:Qizong Lao
Biosynthetic regulation of phospholipids in mammalian cells
哺乳动物细胞中磷脂的生物合成调控
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Kuge;O
- 通讯作者:O
An IkappaB-beta COOH terminal region protein is essential for the proliferation of CHO cells under acidic stress
IkappaB-beta COOH 末端区域蛋白对于酸性胁迫下 CHO 细胞的增殖至关重要
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Kuge;O.;Qizong Lao
- 通讯作者:Qizong Lao
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KUGE Osamu其他文献
KUGE Osamu的其他文献
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{{ truncateString('KUGE Osamu', 18)}}的其他基金
Intracellular transport of phospholipids
磷脂的细胞内运输
- 批准号:
23590080 - 财政年份:2011
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on metabolic control and intracellular transport of phospholipids
磷脂代谢调控及细胞内转运研究
- 批准号:
19590068 - 财政年份:2007
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Biosymthetic Regulation and Intracellular Transport of Phosphatidylserine
磷脂酰丝氨酸的生物合成调节和细胞内运输
- 批准号:
11680644 - 财政年份:1999
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Genetic and Biochemical Study of Phosphatidylserine biosynthesis and its regulation in animal cells
动物细胞磷脂酰丝氨酸生物合成及其调控的遗传与生化研究
- 批准号:
07672411 - 财政年份:1995
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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