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The study on the factors determining the effects of isolation rearing on social behavior in male laboratory mice

隔离饲养对雄性实验小鼠社会行为影响因素的研究

基本信息

批准号：
14510091
负责人：
KATO Katsunori
金额：
$ 2.24万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

Isolation and cohabitation with a female in male residents.ICR male mice were reared in isolation or with a female from seven weeks old. Two weeks later ain truder test was performed. The effects of isolation differed from those of cohabitation with a female.Social experiences and adult social behavior.ICR males were reared from four week old in the following conditions :(1)singly housed, or separated with the partition made of (2)transparent plastic board, (3)small- or (4)large-meshed wire net, or (5)paired. Five week later a social encounter test was performed. Compared with condition (5),aggression increased more in conditions (1)and (2)than in conditions (3)and (4),whereas timid responses increased in conditions (1)-(4). The results suggested that different experiences were involved in aggression and timid responses.Isolation and monoaminergic systems.ICR males were (1)singly housed from four weeks old, (2)paired with a female from seven weeks old, or (3)reared in a group from three weeks old. An encounter test was performed at nine weeks old. Four to seven days later the brains were removed and the contents of monoamines and their metabolites were measured. Isolation reduced monoamines and enhanced the mesolimbic dopaminergic activity. Adrenalin and serotonin reduced largely in the animals dominantly showing aggression or timid responses.Isolation and neural activities during social interaction.ICR males were reared in isolation or in a group from four weeks old. Six to seven weeks later they encountered with another male confined in a small wire-mesh cage, and then the brains were perfused and fixed, c-Fos expression was examined as a measure of neural activity. The result suggested that sensitization especially in the main olfactory pathway and excitability in the amygdala and its projecting regions might be enhanced by isolation.

在雄性居民中隔离和与雌性同居。ICR雄性小鼠从7周龄开始隔离饲养或与雌性小鼠同居。两周后，进行了一项更艰难的测试。从4周龄开始，ICR雄性在以下条件下饲养：(1)单独居住，或用(2)透明塑料板隔断，(3)小-或(4)大网眼铁丝网，或(5)成对饲养。五周后，进行了一项社交接触测试。与条件(5)相比，条件(1)和(2)的攻击性比条件(3)和(4)的增加更多，而胆小反应在条件(1)-(4)中增加。结果表明，不同的经历涉及攻击和胆怯反应、隔离和单胺系统。ICR雄性从4周龄开始单独饲养，(2)从7周龄开始与雌性配对，或(3)从3周龄开始集体饲养。在9周大的时候进行了一次相遇测试。4~7天后取脑，测定单胺类物质及其代谢产物的含量。分离减少了单胺类物质，增强了中脑边缘多巴胺能活性。在这些动物中，肾上腺素和5-羟色胺大量减少，主要表现为攻击性或胆小反应。隔离和社会互动中的神经活动。ICR雄性从四周起单独饲养或成群饲养。六到七周后，他们遇到了另一名被关在一个小铁丝网笼子里的男性，然后对大脑进行灌流和固定，检测c-Fos的表达作为神经活动的衡量标准。结果提示，孤束核可增强杏仁核及其投射区的敏感度，尤其是主要嗅觉通路的敏感度和兴奋性。