The study on the factors determining the effects of isolation rearing on social behavior in male laboratory mice

隔离饲养对雄性实验小鼠社会行为影响因素的研究

基本信息

批准号：
14510091
负责人：
KATO Katsunori
金额：
$ 2.24万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

Isolation and cohabitation with a female in male residents.ICR male mice were reared in isolation or with a female from seven weeks old. Two weeks later ain truder test was performed. The effects of isolation differed from those of cohabitation with a female.Social experiences and adult social behavior.ICR males were reared from four week old in the following conditions :(1)singly housed, or separated with the partition made of (2)transparent plastic board, (3)small- or (4)large-meshed wire net, or (5)paired. Five week later a social encounter test was performed. Compared with condition (5),aggression increased more in conditions (1)and (2)than in conditions (3)and (4),whereas timid responses increased in conditions (1)-(4). The results suggested that different experiences were involved in aggression and timid responses.Isolation and monoaminergic systems.ICR males were (1)singly housed from four weeks old, (2)paired with a female from seven weeks old, or (3)reared in a group from three weeks old. An encounter test was performed at nine weeks old. Four to seven days later the brains were removed and the contents of monoamines and their metabolites were measured. Isolation reduced monoamines and enhanced the mesolimbic dopaminergic activity. Adrenalin and serotonin reduced largely in the animals dominantly showing aggression or timid responses.Isolation and neural activities during social interaction.ICR males were reared in isolation or in a group from four weeks old. Six to seven weeks later they encountered with another male confined in a small wire-mesh cage, and then the brains were perfused and fixed, c-Fos expression was examined as a measure of neural activity. The result suggested that sensitization especially in the main olfactory pathway and excitability in the amygdala and its projecting regions might be enhanced by isolation.

与男性居民中的女性隔离和同居。隔离男性小鼠或七周大的女性。两周后，进行了AIN Truder测试。隔离的影响不同于与女性的同居。在以下条件下，ICR雄性从四个星期大的情况下饲养：（1）单独安装或与（2）透明塑料板的分区分开，（3）小或（4）（4）大或（4）大的金属网或（5）配对。五个星期后，进行了社交遭遇测试。与条件（5）相比，条件（1）和（2）的侵略性比（3）和（4）的条件增加了，而胆怯的反应在条件下增加（1） - （4）。结果表明，侵略和胆怯的反应涉及不同的经历。隔离和单胺能系统。雄性（1）单独容纳了四个星期大，（2）与七个星期大的女性搭配，或者（3）在三个星期大的一组中饲养。在九周大的时间内进行了遭遇测试。四到七天后，去除大脑，并测量单胺及其代谢物的含量。分离减少了单胺并增强了中唇多巴胺能活性。肾上腺素和5-羟色胺在主要表现出侵略性或胆怯的反应的动物中大大降低。在社交互动过程中进行分离和神经活动。孤立或从四个星期大的一组中养育了ICR雄性。六到七个星期后，他们遇到了另一个被限制在小电线笼中的雄性，然后将大脑灌注并固定，C-FOS表达被检查为神经活动的量度。结果表明，尤其是在主要的嗅觉途径中的敏化和杏仁核及其投射区域的兴奋性可能会通过隔离来增强。