Molecular phylogeography of myrmecophyte-ant-scale insect tripartite symbiosis in Southeast Asian tropical forests

东南亚热带森林密生植物-蚁介虫三方共生的分子系统发育地理学

• 批准号: 14540577
• 负责人: ITINO Takao
• 金额: $ 2.3万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540577/
• 关键词: ants; symbiosis; coevolution; ant-plants; molecular phylogeny; mitochondrial DNA; coccids; cospeciation; 共進分化

Many tropical plants house ants and thereby gain protection from herbivores. Most of these myrmecophyte-ant systems include scale insects as the third associate. Scale insects live inside the plant hollow stem, suck the plant sap, and provide honeydew to the ants. We investigate the evolution of the three-way interaction among myrmecophytic Macaranga plants, their inhabitant Crematogaster (Decacrema) ants, and the trophobiont Coccus scale insects. Previous studies show that these three genera have a tight association although a few other genera are sometimes involved.The mtDNA COI phylogenies of comprehensively-sampled ants and scales reveal ten and five well-supported lineages, respectively. Comparisons of the two phylogenies and a published Maearanga phylogeny indicate an absence of cospeciation. Whereas the ant-scale and plant-scale interactions are diffuse, the ant-plant one is specific in the sense that host shifts have been constrained by plant stem traits that are themselves correlated with Macaranga phylogeny. Applying COI divergence rates of 1.5% per My, the Decacrema ants and the Coccus scales from Macaranga are inferred to be at least 12, and 10 My old, respectively, indicating that they have been diversifying contemporaneously since the mid-Miocene in the wet forest community of Southeast Asia.

许多热带植物都栖息着蚂蚁，从而获得免受食草动物侵害的保护。大多数这些密生植物-蚂蚁系统都包括介壳虫作为第三种伙伴。介壳虫生活在植物空心茎内，吸食植物汁液，并向蚂蚁提供蜜露。我们研究了密生植物 Macaranga 植物、其栖息的 Crematogaster (Decacrema) 蚂蚁和营养生物球菌介壳虫之间三向相互作用的进化。先前的研究表明，这三个属具有紧密的关联性，尽管有时也涉及其他一些属。全面采样的蚂蚁和鳞片的 mtDNA COI 系统发育分别揭示了 10 个和 5 个得到充分支持的谱系。两种系统发育和已发表的 Maearanga 系统发育的比较表明不存在共种。尽管蚂蚁-规模和植物-规模的相互作用是分散的，但蚂蚁-植物之间的相互作用是特定的，因为宿主转移受到植物茎性状的限制，而植物茎性状本身与Macaranga系统发育相关。应用每 My 1.5% 的 COI 分歧率，推断来自 Macaranga 的 Decacrema 蚂蚁和 Coccus 鳞片分别至少为 12 My 和 10 My old，表明它们自中新世中期以来在东南亚的湿森林群落中一直在同时多样化。

项目成果

Hatada, A., Itioka, T., Yamaoka, R., Itino, T.: "Carbon and nitrogen contents of food bodies in three myrmecophytic species of Macaranga : implications for antiherbivore defense mechanisms."Journal of Plant Research. 115. 179-184 (2002)

Hatada, A.、Itioka, T.、Yamaoka, R.、Itino, T.：“Macaranga 三种密生植物中食物体的碳和氮含量：对反草食动物防御机制的影响。”植物研究杂志。

Murase, K., Itioka, T., Inui, Y., Itino, T.: "Species specificity in settling-plant selection by foundress ant queens in Macaranga -Crematogaster myrmecophytism in a Bornean dipterocarp forest."Journal of Ethology. 20. 19-24 (2002)

Murase, K.、Itioka, T.、Inui, Y.、Itino, T.：“Macaranga 的始祖蚁后选择定居植物的物种特异性 - 婆罗洲龙脑香科森林中的 Crematogaster myrmecophytism。”动物行为学杂志。

Itino, T., Itioka, T., Davies, S.J.: "Coadaptation and coevolution of Macaranga trees and their symbiotic ants."Genes, Behaviors and Evolution of Social Insects (eds. by Kikuchi, T., Higashi, S., and Azuma, N.)(Hokkaido University Press, Sapporo, Japan).

Itino, T.、Itioka, T.、Davies, S.J.：“Macaranga 树及其共生蚂蚁的共同适应和共同进化。”社会性昆虫的基因、行为和进化（Kikuchi, T.、Higashi, S. 和

Itino, T.: "Coevolution of ants and plants"In : Biodiversity of a Tropical Rainforest and Plant-animal Interaction(eds.by Momose, K., Hamid, A., Roubik, D.W.)Springer, New York. (in press). (2004)

Itino, T.：“蚂蚁和植物的共同进化”，载于：热带雨林的生物多样性和动植物相互作用（由 Momose, K.、Hamid, A.、Roubik, D.W. 编辑）Springer，纽约。

Hatada, A, Itioka, T., Yamaoka, R., Itino, T.: "Carbon and nitrogen contents of food bodies in three myrmecophytic species of Macaranga : implications for antiherbivore defense mechanisms."Journal of Plant Research. 115. 179-184 (2002)

Hatada, A, Itioka, T., Yamaoka, R., Itino, T.：“Macaranga 的三种密生植物中食物体的碳和氮含量：对反草食动物防御机制的影响。”植物研究杂志。