Collaborative Research: Evolutionary genomics of plant-fungal symbiosis: coevolution of Pinaceae and their ectomycorrhizal fungi in the genus Suillus

合作研究：植物-真菌共生的进化基因组学：松科及其外生菌根真菌在猪肝菌属中的共同进化

• 批准号: 1554181
• 负责人: Rytas Vilgalys
• 金额: $ 50.02万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-01 至 2022-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1554181&HistoricalAwards=false
• 关键词: Collaborative; Research; Evolutionary; genomics; plant

Bolete mushrooms (Suillus) are easily recognized fungi that live in close association (symbiosis) with pine tree in forests around the world, and they provide critical roles in the functioning of ecosystems. These ectomycorrhizal fungi provide their plant hosts with resources and protection from pathogens, and in exchange, the mushrooms receive plant-derived sugars. This research will enhance understanding of how these plants and fungi have co-evolved, and this will broadly inform the general area of plant-microbe interactions. The project will increase collaboration among a large network of fungal ecologists and systematists who study biology and ecology of these fungi. Through field collection, knowledge exchange, and participation in scientific workshops, professional networks will be built for scientists from a wide range of academic and industry settings, with attention to underrepresented groups. The research project is geared toward outreach to the general public through partnership with local mushroom clubs and amateur mycologists that will involve lectures, workshops, and participation in field sampling.The co-evolutionary history of Suillus-Pinaceae has not been well studied, which has prevented the full integration of genomics to understand molecular mechanisms of EMF-plant co-evolution. This proposed research will produce a robust and comprehensive molecular phylogeny for the genus Suillus using phylogenomics techniques novel to the field of mycology. Complementary broad sequencing of pine genomes will also provide useful data in which to advance genomics of pines both within and outside of this project. Additional research products will include the generation of least five more genomes for Suillus (only two are currently available) and transcriptomes for multiple members of Suillus and the pines from EMF-colonized roots. Collectively, these molecular resources would be relevant across multiple disciplines: metabolic and signaling networks that participate in co-evolution and host specificity as well as fungal-host recognition, communication, and nutrient acquisition and exchanges. These products will be important for the fields of mycology, botany, ecology, and evolutionary biology.

Bolete蘑菇（Suillus）很容易识别出与世界各地的森林中的松树密切关联（共生）的真菌，它们在生态系统的功能中提供了关键作用。这些外生肌肉真菌为其植物宿主提供了资源和防止病原体的保护，作为交换，蘑菇会接受植物衍生的糖。这项研究将增强对这些植物和真菌如何共同发展的理解，这将广泛地为植物 - 微生物相互作用的一般领域提供信息。该项目将增加研究这些真菌的生物学和生态学的大型真菌生态学家和系统主义者网络之间的合作。通过现场收集，知识交流和参与科学研讨会，将为来自广泛的学术和行业环境的科学家建立专业网络，并关注代表性不足的群体。该研究项目旨在通过与当地蘑菇俱乐部和业余真菌学家的合作伙伴关系，涉及讲座，研讨会和参与现场采样。这项拟议的研究将使用系统基因学领域的系统基因组学技术为Suillus属产生强大而全面的分子系统发育。松树基因组的互补广泛测序还将提供有用的数据，以推动该项目内外的松树基因组学。其他研究产品将包括Suillus的至少五个基因组（目前只有两个）以及为Suillus的多个成员以及EMF殖民化根的PINES的转录组。总的来说，这些分子资源将在多个学科中相关：参与共同进化和宿主特异性的代谢和信号网络以及真菌宿主识别，交流以及营养的获取和交流。这些产品对于真菌学，植物学，生态学和进化生物学的领域至关重要。