Collaborative Research: Evolutionary genomics of plant-fungal symbiosis: coevolution of Pinaceae and their ectomycorrhizal fungi in the genus Suillus

合作研究：植物-真菌共生的进化基因组学：松科及其外生菌根真菌在猪肝菌属中的共同进化

• 批准号: 1554181
• 负责人: Rytas Vilgalys
• 金额: $ 50.02万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-01 至 2022-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1554181&HistoricalAwards=false
• 关键词: Collaborative; Research; Evolutionary; genomics; plant

Bolete mushrooms (Suillus) are easily recognized fungi that live in close association (symbiosis) with pine tree in forests around the world, and they provide critical roles in the functioning of ecosystems. These ectomycorrhizal fungi provide their plant hosts with resources and protection from pathogens, and in exchange, the mushrooms receive plant-derived sugars. This research will enhance understanding of how these plants and fungi have co-evolved, and this will broadly inform the general area of plant-microbe interactions. The project will increase collaboration among a large network of fungal ecologists and systematists who study biology and ecology of these fungi. Through field collection, knowledge exchange, and participation in scientific workshops, professional networks will be built for scientists from a wide range of academic and industry settings, with attention to underrepresented groups. The research project is geared toward outreach to the general public through partnership with local mushroom clubs and amateur mycologists that will involve lectures, workshops, and participation in field sampling.The co-evolutionary history of Suillus-Pinaceae has not been well studied, which has prevented the full integration of genomics to understand molecular mechanisms of EMF-plant co-evolution. This proposed research will produce a robust and comprehensive molecular phylogeny for the genus Suillus using phylogenomics techniques novel to the field of mycology. Complementary broad sequencing of pine genomes will also provide useful data in which to advance genomics of pines both within and outside of this project. Additional research products will include the generation of least five more genomes for Suillus (only two are currently available) and transcriptomes for multiple members of Suillus and the pines from EMF-colonized roots. Collectively, these molecular resources would be relevant across multiple disciplines: metabolic and signaling networks that participate in co-evolution and host specificity as well as fungal-host recognition, communication, and nutrient acquisition and exchanges. These products will be important for the fields of mycology, botany, ecology, and evolutionary biology.

牛肝菌（Suillus）是一种很容易识别的真菌，与世界各地森林中的松树密切相关（共生），它们在生态系统的功能中发挥着关键作用。这些外生菌根真菌为它们的植物宿主提供资源和免受病原体侵害的保护，作为交换，蘑菇获得植物来源的糖。这项研究将加深对这些植物和真菌如何共同进化的理解，这将广泛地为植物-微生物相互作用的一般领域提供信息。该项目将增加研究这些真菌的生物学和生态学的真菌生态学家和系统学家的大型网络之间的合作。通过实地收集、知识交流和参加科学讲习班，将为来自广泛学术和工业背景的科学家建立专业网络，并关注代表性不足的群体。该研究项目是面向推广到广大公众通过与当地蘑菇俱乐部和业余真菌学家的合作伙伴关系，将涉及讲座，研讨会，并在现场sampling.The共同进化的历史Suillus-Pinaceae没有得到很好的研究，这已经阻止了基因组学的全面整合，以了解电磁场植物共同进化的分子机制。这项拟议的研究将产生一个强大的和全面的分子系统学属猪乳牛肝菌使用真菌学领域的新的基因组学技术。松树基因组的互补性广泛测序也将提供有用的数据，以推进该项目内外的松树基因组学。额外的研究产品将包括为Suillus产生至少五个基因组（目前只有两个可用）和多个Suillus成员的转录组以及来自EMF殖民根的松树。总的来说，这些分子资源将与多个学科相关：参与共同进化和宿主特异性以及真菌-宿主识别，通信和营养物质获取和交换的代谢和信号网络。这些产品对真菌学、植物学、生态学和进化生物学领域都很重要。