Molecular and cellular diversity of aquaporin in a water-adaptation of the amphibians

两栖动物水适应中水通道蛋白的分子和细胞多样性

基本信息

  • 批准号:
    14540612
  • 负责人:
  • 金额:
    $ 2.3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2003
  • 项目状态:
    已结题

项目摘要

We cloned cDNAs encoding three distinct AQPs (AQP-h1,AQP-h2 and AQP-h3) from the ventral skin of the tree frog, Hyla japonica. AQP-h1 mRNA is expressed in a wide variety of tissues, and AQP-h2 in several tissues including anti-diuretic hormone-dependent tissues, but AQP-h3 mRNA exists only in the ventral skin. An immunofluorescence study showed that the AQP-h3 protein was only localized in the pelvic ventral skin. In the pelvic skin, AQP-h3 protein was more intense in the upper layer of the stratum granulosum, and was localized in both the apical and basolateral plasma membranes of the granular cells. AQP-h3 in the ventral skin may be essential in the land adaptation of the tree frog, but AQP-h2 protein was detected in the same sites as AQP-h3 in the plasma membrane of the granular cells in the adult ventral skin. In the urinary bladder, AQP-h2 protein was observed in the cytoplasm of the granular cells. Incubation of adult ventral skin with vasotocin enhanced the labeling density of both AQP-h2 and AQP-h3 in the apical plasma membrane in the skin. Taken together, the present data suggest that AQP-h3 is an intrinsic AQP in the ventral skin, and that it maintains a high permeability to water in the ventral skin in concert with AQP-h2 under the regulation of vasotocin. In the urinary bladder, AQP-h2 plays a pivotal role in the reabsorption of water. It is of interest that both AQP-h2-and AQP-h3-like proteins are expressed in the ventral skin of the terrestrial frog Bufo japonica and tree-adapted frog Hyla japonica. On the other hand, expression of AQP-h3-like gene in the ventral skins is observed in all the frogs from aquatic species to terrestrial dwellers, but no AQP-h2-like protein is expressed in the ventral skin in the aquatic and semi-terrestrial-adapted frogs.
我们从树蛙腹侧皮肤中克隆了编码三种不同的水通道蛋白(AQP-h1、AQP-h2和AQP-h3)的cDNA。AQP-H1mRNA在多种组织中表达,AQP-H2在几种组织中表达,包括抗利尿激素依赖的组织,但AQP-h3mRNA仅存在于腹侧皮肤。免疫荧光研究表明,AQP-h3蛋白仅定位于盆腔腹侧皮肤。在盆腔皮肤中,AQP-h3蛋白在颗粒层上层表达较强,并定位于颗粒细胞的顶端和基底外侧质膜。腹侧皮肤AQP-h3可能是树蛙陆地适应所必需的,但AQP-H2蛋白与成体腹侧皮肤颗粒细胞质膜上的AQP-h3蛋白存在于同一部位。在膀胱中,AQP-H2蛋白定位于颗粒细胞的胞浆中。成人腹侧皮肤与血管催产素孵育后,AQP-h2和AQP-h3在皮肤顶端质膜上的标记密度均增加。综上所述,本研究结果提示AQP-h3是腹侧皮肤固有的AQP,在血管催产素的调节下与AQP-H2共同维持腹侧皮肤对水的高通透性。在膀胱中,AQP-H2在水的重吸收中起着关键作用。有趣的是,AQP-h2和AQP-h3样蛋白在陆生蛙和树适应蛙的腹侧皮肤中都有表达。另一方面,AQP-h3类基因在从水生生物到陆栖动物的所有蛙类的腹面皮肤中都有表达,但在水生和半陆地适应的青蛙腹面皮肤中没有表达AQP-H2类蛋白。

项目成果

期刊论文数量(47)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yuki Tajika: "Immunohistochemical characterization of the intracellular pool of water channel aquaporin-2 in the kidney., 2002."Anatomical Science International. 77. 189-195
Yuki Tajika:“肾脏水通道 aquaporin-2 细胞内池的免疫组织化学特征。,2002 年。”国际解剖科学。
  • DOI:
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    0
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Reiko Okada: "Development of radioimmunoassay for bullfrog thyroid-stimulating hormone (TSH) : effects of hypothalamic releasing hormones on the release of TSH from the pituitary in vitro Involvement of arginine vasotocin in reproductive events in the mal
Reiko Okada:“牛蛙促甲状腺激素 (TSH) 放射免疫测定法的开发:下丘脑释放激素对体外垂体释放 TSH 的影响精氨酸加压素在马生殖事件中的作用
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    0
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Yuki Tajika: "Immunohistochemical characterization of the intracellular pool of water channel aquaporin-2 in the kidney."Anatomical Science International. 77. 189-195 (2002)
Yuki Tajika:“肾脏水通道 aquaporin-2 细胞内池的免疫组织化学特征。”国际解剖科学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Shigeyasu Tanaka: "Comparative aspects of intercellular proteolytic processing of peptide hormone precursors : studies of proopiomelanocortin processing."Zoological Science. 20. 1183-1198 (2003)
Shigeyasu Tanaka:“肽激素前体细胞间蛋白水解加工的比较方面:阿片黑皮素原加工的研究。”动物学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yuichi Yaoi: "Expression and localization of prohormone convertase PC1 in the calcitonin-producing cells of the bullfrog ultimobranchial gland."Journal of Histochemistry and Cytochemistry. 51. 1459-1466 (2003)
Yuichi Yaoi:“牛蛙上鳃腺降钙素生成细胞中激素原转化酶 PC1 的表达和定位。”组织化学和细胞化学杂志。
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    0
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TANAKA Shigeyasu其他文献

TANAKA Shigeyasu的其他文献

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{{ truncateString('TANAKA Shigeyasu', 18)}}的其他基金

Development of dopant concentration measuring technique for next generation semiconductor devices
开发下一代半导体器件的掺杂剂浓度测量技术
  • 批准号:
    25420285
  • 财政年份:
    2013
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of dopant concentration measurement technique for next-generation devices
开发下一代器件的掺杂剂浓度测量技术
  • 批准号:
    20560296
  • 财政年份:
    2008
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Water adaptation strategy in anuran amphibians and molecular diversity of aquaporin
无尾两栖动物的水适应策略和水通道蛋白的分子多样性
  • 批准号:
    19370024
  • 财政年份:
    2007
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Role of vascular endothelial growth factor (VEGF) in the formation of vascular system for regulating the adenohyphysial function
血管内皮生长因子(VEGF)在血管系统形成中调节腺垂体功能的作用
  • 批准号:
    12640649
  • 财政年份:
    2000
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Cellular organization and neuronal inducer (inhibitor) in Xenopus adenohypothesis.
非洲爪蟾腺假说中的细胞组织和神经元诱导剂(抑制剂)。
  • 批准号:
    08640841
  • 财政年份:
    1996
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Variation and immunocyochemical localization of processing enzymes in proopiomelanocortin cells during ontoneny
原阿黑皮素细胞在色调过程中加工酶的变化和免疫细胞化学定位
  • 批准号:
    04670002
  • 财政年份:
    1992
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Immunoelectron microscopical analysis of intracellular processing of hormones by use of ultracryosection.
使用超冷冻切片对细胞内激素加工进行免疫电子显微镜分析。
  • 批准号:
    02670004
  • 财政年份:
    1990
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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    20K15895
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