慢性関節リウマチ滑膜におけるリンパ濾胞の免疫学的検討

类风湿性关节炎滑膜淋巴滤泡的免疫学研究

• 批准号: 14770753
• 负责人: 竹村 清介
• 金额: $ 2.05万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14770753/
• 关键词: 関節リウマチ; リンパ濾胞; 免疫染色; 滑膜; HLA; 免疫組織染色

関節リウマチでは種々の免疫担当細胞が滑膜に浸潤し,主としてT細胞とB細胞により構成されるリンパ球集塊を形成することがある.リウマチ滑膜に見られるリンパ球集塊は,その中に濾胞樹状細胞を有するfollicular synovitisとそれを有さないaggregate synovitisに分類される.この二群の滑膜炎を免疫組織学的に比較検討した.アメリカリウマチ学会の関節リウマチの診断基準を満たす関節リウマチ患者23人,29関節の関節滑膜のパラフィン切片を使用した.滑膜の免疫組織染色(CD23)によりfollicular synovitis (F群)とaggregate synovitis(A群)に分類した.F群ではCD20陽性B細胞がその中心に凝集し,CD4陽性T細胞がそれを覆うように分布していた.このリンパ球集塊はリンパ節などの二次性リンパ器官にみられる胚中心に酷似した微細構造を形成していた.これに対してA群では,B細胞とCD4陽性T細胞が無秩序に分布していた.CD8陽性T細胞に関しては,両群ともにリンパ球集塊の周囲に散在するように分布していた.F群では主としてB細胞よりlymphotoxin (LT) βが産生されていたが,A群ではLTβ産生細胞は確認できなかった.新生血管の増生に関しては,二群間に有意差は認められなかった.関節リウマチ滑膜に見られるリンパ球集塊は,胚中心に酷似した微細構造を有するfollicular synovitisとそれを有さないaggregate synovitisの二群に分類される.これらの滑膜炎は酷似した組織学上同じ様相を呈しているが,免疫組織学的には全く異なった範疇に分類された.

The synovial membrane is infiltrated by immune cells, and T cells and B cells are formed into spherical aggregates. The synovial membrane is classified as follicular synovitis and aggregate synovitis. A comparative study of immunohistology of synovitis in two groups. Joint synovial sections from 23 patients were used. Immunohistochemical staining of synovium (CD23) classified as follicular synovitis (group F) and aggregate synovitis(group A). CD20-positive B cells in group F were aggregated in the center, CD4-positive T cells were distributed in the center. The spherical mass is divided into two parts: the first part is divided into two parts: the second part is divided into two parts: the first part is divided into three parts: the first part is divided into three parts: the second part is divided into three parts: the first part is divided into three parts: the first part is divided into four parts: the second part is divided into three parts: the first part is divided into four parts: the first part is divided into four parts: the second part is divided into four parts: the first part is divided into four parts: the first part is divided into four parts: the second part is divided into four parts: the first part is divided into four parts: the first part is divided into four parts: the second part is divided into four parts: the first part is divided into four parts: the second part is divided into four parts: the third part is divided into four parts: the third part is divided into four parts: the fourth part is divided into four In contrast,B cells and CD4 positive T cells in group A were randomly distributed. CD8 positive T cells in group B were distributed randomly. In group F, B cells and LT beta producing cells were mainly distributed. In group A, LT beta producing cells were confirmed. The growth of new blood vessels is related to the growth of new blood vessels. Joint synovium is classified into two groups: follicular synovitis and aggregate synovitis. The synovitis is histologically similar, but the immunohistologic classification is totally different.

项目成果

Seisuke Takemura, et al.: "HLA-DRB1 haplotype did not affect the middle-term results of total knee arthroplasty in patients with rheumatoid arthritis."Modern Rheumatology. (In press). (2004)

Seisuke Takemura等人：“HLA-DRB1单倍型并不影响类风湿性关节炎患者全膝关节置换术的中期结果。”现代风湿病学。