The role of the Aspm (Abnormal spindle microcephaly-associated) protein in symmetric versus asymmetric division of polarized neuroepithelial cells.

Aspm（异常纺锤体小头畸形相关）蛋白在极化神经上皮细胞对称与不对称分裂中的作用。

• 批准号: 5332940
• 负责人: Professor Dr. Wieland B. Huttner
• 金额: --
• 依托单位: Max-Planck-Institut für molekulare Zellbiologie und Genetik (MPI-CBG)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5332940?language=en
• 关键词: role; Aspm; Abnormal; spindle; microcephaly

Neuroepithelial cells, which give rise to all neurons of the mammalian central nervous system, do so by switching from symmetric, proliferative to asymmetric, neuron-generating division. The cell biological basis of this switch is unknown. However, extrapolating from the results of classical studies in Drosophila, the apical-basal polarity of mammalian neuroepithelial cells is likely to be of key importance for their asymmetric division. However, for mammalian neuroepithelial cells, very little is known about proteins of apical plasma membrane and their association with specific, cholesterol-based membrane microdomains, which in non-neural model cells have been shown to be crucial for signal transduction. It is therefore proposed to (i) identify apical plasma membrane proteins of mouse neuroepithelial cells, (ii) characterize their association with specific lipid microdomains using novel tools developed in our group, and (iii) investigate the regulation of these proteins and their association with lipid microdomains as neuroepithelial cells switch from symmetric, proliferative to asymmetric, neuron-generating division.

神经上皮细胞产生哺乳动物中枢神经系统的所有神经元，通过从对称的、增殖的分裂转变为不对称的、产生神经元的分裂来实现。这种转换的细胞生物学基础尚不清楚。然而，从果蝇的经典研究结果推断，哺乳动物神经上皮细胞的顶端-基底极性可能对其不对称分裂至关重要。然而，对于哺乳动物神经上皮细胞，很少有人知道顶端质膜的蛋白质及其与特定的，胆固醇为基础的膜微区，这在非神经模型细胞已被证明是至关重要的信号转导。因此，建议（i）确定小鼠神经上皮细胞的顶端质膜蛋白，（ii）使用我们小组开发的新工具表征它们与特定脂质微结构域的关联，以及（iii）研究这些蛋白质的调节及其与脂质微结构域的关联，因为神经上皮细胞从对称，增殖到不对称，神经元生成分裂。