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Understanding of the circadian system

了解昼夜节律系统

基本信息

批准号：
15109004
负责人：
HONMA Ken-ichi
金额：
$ 72.3万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (S)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2007
项目状态：
已结题

项目摘要

The purpose of the preset study is to understand the functions of circadian clock in mammals as the multiple oscillator system from the molecular and cellular levels to behaviors.1. Molecular mechanism of circadian system in the suprachiasmatic nucleus (SCN)1) Cellular levels: The dynamics of the feedback loop consisting of clock genes, Per1, 2, Cry1, 2, Bmal1, and their protein product (core loop), the coupling of the Decl, Dec2 associate loop, the function of Bmal1 loop coupling with the core loop, and the output mechanism of core oscillating loop were elucidated.2) Tissue levels: The responses of circadian rhythms in Perl, 2, Decl, Dec2 expressions to photic stimulations in the whole area of SCN and the photoperiodic responses of the circadian rhythms in Per1, 2 expressions in the whole SCN areas were elucidated.2. Circadian oscillating system in the SCNThe circadian gene expression rhythms in the culture SCN under Tetrodotoxin treatment, the functions of circadian rhythm in the SCN … More from newborn mice in which the synaptic communications are immature, the photoperiodic responses of the circadian clock gene expression rhythms in the single SCN cells3. The circadian systems in the SCN and peripheral clocks were elucidated.1) Behavioral levels: The circadian behavioral rhythms and the SCN and peripheral circadian systems in the histamine synthetic enzyme knockout mice, the circadian clock gene expression rhythms in the discrete areas of brain in the methamphetamine treated mice, the differences in the non-photic entrainment of the central and peripheral clocks were elucidated.2) Tissue levels: The mechanism of Dexamethasone-induced circadian rhythm, the auto-feedback regulation in the peripheral circadian system, the responses of auto-feedback loop in the peripheral clock to the over-expression of clock gene were elucidated.4. Introduction of a bioluminescence reporter system for clock gene expressions into miceTransgenic mice were produced in which bioluminescence reporter system for Per1 and Bmal1 were produced. Less

本研究的目的是从分子水平、细胞水平和行为水平了解哺乳动物生物钟作为多振子系统的功能.视交叉上核（SCN）昼夜节律系统的分子机制1）细胞水平：时钟基因Per1，2，Cry1，2，Bmal1及其蛋白产物组成的反馈环的动力学（核心回路）、Dec1、Dec2关联回路的耦合、Bmal1回路与核心回路耦合的功能，并阐明了核心振荡回路的输出机制。2）组织水平：Per1，2，Dec1，Dec2表达的昼夜节律对SCN全区光刺激的反应和Per1，2阐明了SCN全脑区的表达. SCN中的昼夜节律振荡系统河豚毒素作用下培养SCN中基因表达的昼夜节律， ...更多信息来自突触通讯不成熟的新生小鼠，单个SCN细胞中生物钟基因表达节律的光周期反应3。阐明了SCN和外周生物钟的昼夜节律系统。1）行为水平：阐明了组胺合成酶基因敲除小鼠的昼夜行为节律和SCN及外周生物钟系统，甲基苯丙胺处理小鼠脑离散区域的昼夜生物钟基因表达节律，中枢和外周生物钟的非光诱导差异。2）组织水平：阐明地塞米松诱导昼夜节律的机制，外周昼夜节律系统的自反馈调节，外周时钟的自反馈环对时钟基因过表达的反应.将生物发光报告系统导入小鼠体内，建立了转基因小鼠，并在转基因小鼠中制备了Per1和Bmal 1的生物发光报告系统。少