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Synthesis of Biohybrid Gels That Respond to Signal Biomolecules and Their Biomedical Applications

响应信号生物分子的生物混合凝胶的合成及其生物医学应用

基本信息

批准号：
15300173
负责人：
MIYATA Takashi
金额：
$ 10.56万
依托单位：
Kansai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

In this study, we tried to prepare (a) biomolecule-crosslinked gels and (b) biomolecule-imprinted gels as biohybrid gels that undergo volume changes in response to signal biomolecules :(a) Biomolecule-crosslinked gelsReversibly glucose-responsive gels were prepared by using complex formation between lectins and polymers with pendant glucose. Biohybrid gels having antigen-antibody complexes as crosslinking points swelled immediately in the presence of a target antigen and shrank again in its absence. The antigen-responsive gels controlled the drug permeation in response to the antigen concentration. DNA-responsive gels were prepared by introducing duplex DNA at crosslinking points. Swelling behavior of the duplex DNA-crosslinked gels was strongly dependent upon the sequence of DNA. Measurements of crosslinking density of their biohybrid gels revealed that their biomolecule-responsive swelling is due to the dissociation of the biomolecular complexes in the presence of a target biomolecul … More e.(b) Biomolecule-imprinted gelsGlycoprotein-imprinted gels were prepared by biomolecular imprinting in which lectin and antibody were utilized as ligands for a target tumor-marker glycoprotein. The glycoprotein-imprinted gels only shrank when both lectin and antibody in the gels simultaneously recognized the saccharide and peptide chains of the target glycoprotein. The glycoprotein-responsive shrinking of the imprinted gel was due to formation of lectin-glycoprotein-antibody complexes that acted as reversible crosslinking points. Furthermore, DNA-imprinted gels having two ligand DNAs shrank in a buffer solution containing a target DNA but swelled a little in the presence of DNAs with different sequences. The responsive shrinking behavior of the bimolecule-imprinted gels is attributed to an increase in their crosslinking density by complex formation between ligands and target biomolecules.As biomolecule-responsive behavior of biomolecule-crosslinked gels and biomolecule-imprinted gels enables the accurate detection and recognition of target biomolecules, they have many potential applications as smart devices in sensing systems and as molecular diagnostics. Less

在本研究中，我们尝试制备（a）生物分子交联凝胶和（B）生物分子印迹凝胶作为响应信号生物分子而发生体积变化的生物杂化凝胶：（a）生物分子交联凝胶通过利用凝集素和具有侧链葡萄糖的聚合物之间的复合物形成来制备可逆葡萄糖响应凝胶。具有抗原-抗体复合物作为交联点的生物杂交凝胶在靶抗原存在时立即膨胀，在靶抗原不存在时再次收缩。抗原响应性凝胶响应于抗原浓度控制药物渗透。通过在交联点引入双链体DNA制备DNA响应性凝胶。双链DNA交联凝胶的溶胀行为强烈依赖于DNA的序列。对它们的生物杂化凝胶的交联密度的测量表明，它们的生物分子响应性溶胀是由于在靶生物分子存在下生物分子复合物的解离。 ...更多信息 e. (b)生物分子印迹凝胶以凝集素和抗体为配体，通过生物分子印迹技术制备了糖蛋白印迹凝胶。只有当凝胶中的凝集素和抗体同时识别目标糖蛋白的糖链和肽链时，糖蛋白印迹凝胶才会收缩。印迹凝胶的糖蛋白响应性收缩是由于凝集素-糖蛋白-抗体复合物的形成，该复合物充当可逆交联点。此外，具有两个配体DNA的DNA印迹凝胶在含有靶DNA的缓冲溶液中收缩，但在具有不同序列的DNA存在下略微溶胀。双分子印迹凝胶的响应性收缩行为归因于配体与目标生物分子之间形成络合物而增加其交联密度，生物分子交联凝胶和生物分子印迹凝胶的生物分子响应性行为使得能够准确地检测和识别目标生物分子，因此它们在传感系统的智能器件和分子诊断方面具有许多潜在的应用。少