Synthesis of Biohybrid Gels That Respond to Signal Biomolecules and Their Biomedical Applications

在这项研究中，我们试图制备(a)生物分子交联凝胶和(b)生物分子印迹凝胶作为响应信号生物分子而发生体积变化的生物杂交凝胶：(a)生物分子交联凝胶通过利用凝集素和聚合物与悬垂葡萄糖之间形成复合物制备可逆葡萄糖反应凝胶。具有抗原-抗体复合物作为交联点的生物杂交凝胶在靶抗原存在时立即膨胀，在靶抗原不存在时再次收缩。抗原反应凝胶根据抗原浓度控制药物渗透。通过在交联点引入双链DNA制备DNA反应凝胶。双链DNA交联凝胶的膨胀行为强烈依赖于DNA的序列。他们的生物杂交凝胶的交联密度测量显示，其生物分子反应性肿胀是由于生物分子复合物在目标生物分子存在下解离所致。更多e.(b)生物分子印迹凝胶糖蛋白印迹凝胶是通过生物分子印迹制备的，其中凝集素和抗体作为靶肿瘤标记糖蛋白的配体。当凝胶中的凝集素和抗体同时识别目标糖蛋白的糖链和肽链时，糖蛋白印迹凝胶才会收缩。印迹凝胶的糖蛋白响应性收缩是由于凝集素-糖蛋白-抗体复合物的形成，作为可逆交联点。此外，具有两个配体DNA的DNA印迹凝胶在含有目标DNA的缓冲溶液中收缩，但在具有不同序列的DNA存在时略微膨胀。双分子印迹凝胶的响应性收缩行为是由于配体和靶生物分子之间形成的络合物增加了它们的交联密度。由于生物分子交联凝胶和生物分子印迹凝胶的生物分子响应行为能够准确地检测和识别目标生物分子，因此它们在传感系统和分子诊断方面具有许多潜在的应用前景。少

In this study, we tried to prepare (a) biomolecule-crosslinked gels and (b) biomolecule-imprinted gels as biohybrid gels that undergo volume changes in response to signal biomolecules :(a) Biomolecule-crosslinked gelsReversibly glucose-responsive gels were prepared by using complex formation between lectins and polymers with pendant glucose. Biohybrid gels having antigen-antibody complexes as crosslinking points swelled immediately in the presence of a target antigen and shrank again in its absence. The antigen-responsive gels controlled the drug permeation in response to the antigen concentration. DNA-responsive gels were prepared by introducing duplex DNA at crosslinking points. Swelling behavior of the duplex DNA-crosslinked gels was strongly dependent upon the sequence of DNA. Measurements of crosslinking density of their biohybrid gels revealed that their biomolecule-responsive swelling is due to the dissociation of the biomolecular complexes in the presence of a target biomolecul … More e.(b) Biomolecule-imprinted gelsGlycoprotein-imprinted gels were prepared by biomolecular imprinting in which lectin and antibody were utilized as ligands for a target tumor-marker glycoprotein. The glycoprotein-imprinted gels only shrank when both lectin and antibody in the gels simultaneously recognized the saccharide and peptide chains of the target glycoprotein. The glycoprotein-responsive shrinking of the imprinted gel was due to formation of lectin-glycoprotein-antibody complexes that acted as reversible crosslinking points. Furthermore, DNA-imprinted gels having two ligand DNAs shrank in a buffer solution containing a target DNA but swelled a little in the presence of DNAs with different sequences. The responsive shrinking behavior of the bimolecule-imprinted gels is attributed to an increase in their crosslinking density by complex formation between ligands and target biomolecules.As biomolecule-responsive behavior of biomolecule-crosslinked gels and biomolecule-imprinted gels enables the accurate detection and recognition of target biomolecules, they have many potential applications as smart devices in sensing systems and as molecular diagnostics. Less