Synthesis of Biohybrid Gels That Respond to Signal Biomolecules and Their Biomedical Applications

响应信号生物分子的生物混合凝胶的合成及其生物医学应用

基本信息

批准号：
15300173
负责人：
MIYATA Takashi
金额：
$ 10.56万
依托单位：
Kansai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

In this study, we tried to prepare (a) biomolecule-crosslinked gels and (b) biomolecule-imprinted gels as biohybrid gels that undergo volume changes in response to signal biomolecules :(a) Biomolecule-crosslinked gelsReversibly glucose-responsive gels were prepared by using complex formation between lectins and polymers with pendant glucose. Biohybrid gels having antigen-antibody complexes as crosslinking points swelled immediately in the presence of a target antigen and shrank again in its absence. The antigen-responsive gels controlled the drug permeation in response to the antigen concentration. DNA-responsive gels were prepared by introducing duplex DNA at crosslinking points. Swelling behavior of the duplex DNA-crosslinked gels was strongly dependent upon the sequence of DNA. Measurements of crosslinking density of their biohybrid gels revealed that their biomolecule-responsive swelling is due to the dissociation of the biomolecular complexes in the presence of a target biomolecul … More e.(b) Biomolecule-imprinted gelsGlycoprotein-imprinted gels were prepared by biomolecular imprinting in which lectin and antibody were utilized as ligands for a target tumor-marker glycoprotein. The glycoprotein-imprinted gels only shrank when both lectin and antibody in the gels simultaneously recognized the saccharide and peptide chains of the target glycoprotein. The glycoprotein-responsive shrinking of the imprinted gel was due to formation of lectin-glycoprotein-antibody complexes that acted as reversible crosslinking points. Furthermore, DNA-imprinted gels having two ligand DNAs shrank in a buffer solution containing a target DNA but swelled a little in the presence of DNAs with different sequences. The responsive shrinking behavior of the bimolecule-imprinted gels is attributed to an increase in their crosslinking density by complex formation between ligands and target biomolecules.As biomolecule-responsive behavior of biomolecule-crosslinked gels and biomolecule-imprinted gels enables the accurate detection and recognition of target biomolecules, they have many potential applications as smart devices in sensing systems and as molecular diagnostics. Less

In this study, we tried to prepare (a) biomolecule-crosslinked gels and (b) biomolecule-imprinted gels as biohybrid gels that undergo volume changes in response to signal biomolecules :(a) Biomolecule-crosslinked gels Reversibly glucose-responsive gels were prepared by using complex formation between levers and polymers with pendant glucose.具有抗原抗体复合物的生物杂化凝胶，因为在靶抗原存在下，交联点立即膨胀，并在没有靶抗原的情况下再次收缩。抗原反应性凝胶响应抗原浓度控制了药物渗透。通过在交联点引入双链DNA来制备DNA响应性凝胶。双链DNA交联凝胶的肿胀行为很大程度上取决于DNA的序列。其生物杂化凝胶的交联密度的测量表明，它们的生物分子反应性肿胀是由于在存在靶标生物分子的存在下生物分子复合物的解离，而更多的生物分子……（b）生物分子 - 分子 - 分子 - 含量质蛋白质 - 固定型固定在其固定的凝胶固有的凝胶固有的凝胶固定在于饲养的固定型固定在于饲养的凝胶固有的凝胶，作为靶肿瘤标记糖蛋白的配体。只有当凝胶中的杠杆和抗体均简单地识别靶糖蛋白的糖和胡椒链时，糖蛋白的透明凝胶才会缩减。印迹凝胶的糖蛋白响应性收缩是由于形成了糖糖蛋白抗体复合物，该复合物充当可逆交联点。此外，在包含靶DNA的缓冲液中收缩了两个配体DNAS的DNA刻度凝胶，但在具有不同序列的DNA的情况下膨胀了一点。双子分子压印凝胶的响应萎缩行为归因于通过配体和靶性生物分子之间的复杂形成通过复杂的形成来增加其交联密度。由于生物分子反应性行为的生物分子反应性行为是生物分子 - 分子互联凝胶和生物分子式验证的质量识别的精确范围，并识别其精确的识别，并具有准确的识别，并识别了可观的探测，并构成了识别且能够识别的可观且可识别的可构成且识别的可观且可识别且可观的识别且可观的识别且可观的识别。作为传感系统中的智能设备和分子诊断。较少的