Synthesis of Biohybrid Gels That Respond to Signal Biomolecules and Their Biomedical Applications

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In this study, we tried to prepare (a) biomolecule-crosslinked gels and (b) biomolecule-imprinted gels as biohybrid gels that undergo volume changes in response to signal biomolecules :(a) Biomolecule-crosslinked gelsReversibly glucose-responsive gels were prepared by using complex formation between lectins and polymers with pendant glucose. Biohybrid gels having antigen-antibody complexes as crosslinking points swelled immediately in the presence of a target antigen and shrank again in its absence. The antigen-responsive gels controlled the drug permeation in response to the antigen concentration. DNA-responsive gels were prepared by introducing duplex DNA at crosslinking points. Swelling behavior of the duplex DNA-crosslinked gels was strongly dependent upon the sequence of DNA. Measurements of crosslinking density of their biohybrid gels revealed that their biomolecule-responsive swelling is due to the dissociation of the biomolecular complexes in the presence of a target biomolecul … More e.(b) Biomolecule-imprinted gelsGlycoprotein-imprinted gels were prepared by biomolecular imprinting in which lectin and antibody were utilized as ligands for a target tumor-marker glycoprotein. The glycoprotein-imprinted gels only shrank when both lectin and antibody in the gels simultaneously recognized the saccharide and peptide chains of the target glycoprotein. The glycoprotein-responsive shrinking of the imprinted gel was due to formation of lectin-glycoprotein-antibody complexes that acted as reversible crosslinking points. Furthermore, DNA-imprinted gels having two ligand DNAs shrank in a buffer solution containing a target DNA but swelled a little in the presence of DNAs with different sequences. The responsive shrinking behavior of the bimolecule-imprinted gels is attributed to an increase in their crosslinking density by complex formation between ligands and target biomolecules.As biomolecule-responsive behavior of biomolecule-crosslinked gels and biomolecule-imprinted gels enables the accurate detection and recognition of target biomolecules, they have many potential applications as smart devices in sensing systems and as molecular diagnostics. Less