Synthesis of Biohybrid Gels That Respond to Signal Biomolecules and Their Biomedical Applications

在这项研究中，我们试图制备(A)生物分子交联凝胶和(B)生物分子印迹凝胶作为生物杂化凝胶，其体积随信号生物分子的变化而发生变化：(A)生物分子交联凝胶利用凝集素与含有侧链葡萄糖的聚合物之间的络合反应制备可逆的葡萄糖响应凝胶。以抗原-抗体复合体为交联点的生物杂化凝胶在靶抗原存在的情况下立即膨胀，在没有靶抗原的情况下再次收缩。抗原响应性凝胶控制药物对抗原浓度的渗透。通过在交联点引入双链DNA制备了DNA响应性凝胶。双链DNA交联凝胶的溶胀行为强烈依赖于DNA序列。生物杂化凝胶的交联度测量表明，它们的生物分子响应性溶胀是由于目标生物分子…存在下生物分子复合体的解离所致。生物分子印迹凝胶以凝集素和抗体为靶向肿瘤标志物糖蛋白的配体，通过生物分子印迹法制备了糖蛋白印迹凝胶。只有当凝胶中的凝集素和抗体同时识别目标糖蛋白的糖链和肽链时，糖蛋白印迹凝胶才会收缩。印迹凝胶的糖蛋白反应性收缩是由于凝集素-糖蛋白-抗体复合体的形成，凝集素-糖蛋白-抗体复合体作为可逆交联点。此外，含有两个配体DNA的DNA印迹凝胶在含有目标DNA的缓冲溶液中收缩，但在存在不同序列的DNA时略有膨胀。双分子印迹凝胶的响应性收缩行为归因于配体与目标生物分子之间形成的络合物增加了其交联密度。由于生物分子交联凝胶和生物分子印迹凝胶的生物分子响应行为使得对目标生物分子的准确检测和识别成为可能，它们在传感系统和分子诊断方面具有许多潜在的应用前景。较少

In this study, we tried to prepare (a) biomolecule-crosslinked gels and (b) biomolecule-imprinted gels as biohybrid gels that undergo volume changes in response to signal biomolecules :(a) Biomolecule-crosslinked gelsReversibly glucose-responsive gels were prepared by using complex formation between lectins and polymers with pendant glucose. Biohybrid gels having antigen-antibody complexes as crosslinking points swelled immediately in the presence of a target antigen and shrank again in its absence. The antigen-responsive gels controlled the drug permeation in response to the antigen concentration. DNA-responsive gels were prepared by introducing duplex DNA at crosslinking points. Swelling behavior of the duplex DNA-crosslinked gels was strongly dependent upon the sequence of DNA. Measurements of crosslinking density of their biohybrid gels revealed that their biomolecule-responsive swelling is due to the dissociation of the biomolecular complexes in the presence of a target biomolecul … More e.(b) Biomolecule-imprinted gelsGlycoprotein-imprinted gels were prepared by biomolecular imprinting in which lectin and antibody were utilized as ligands for a target tumor-marker glycoprotein. The glycoprotein-imprinted gels only shrank when both lectin and antibody in the gels simultaneously recognized the saccharide and peptide chains of the target glycoprotein. The glycoprotein-responsive shrinking of the imprinted gel was due to formation of lectin-glycoprotein-antibody complexes that acted as reversible crosslinking points. Furthermore, DNA-imprinted gels having two ligand DNAs shrank in a buffer solution containing a target DNA but swelled a little in the presence of DNAs with different sequences. The responsive shrinking behavior of the bimolecule-imprinted gels is attributed to an increase in their crosslinking density by complex formation between ligands and target biomolecules.As biomolecule-responsive behavior of biomolecule-crosslinked gels and biomolecule-imprinted gels enables the accurate detection and recognition of target biomolecules, they have many potential applications as smart devices in sensing systems and as molecular diagnostics. Less