The effects of three-dimensional form on the healing process of three-dimensional alveolar bone defect.

本研究的目的是阐明牙周组织再生能力的修复过程，建立牙周组织再生能力的评价标准。实验动物为雄性食蟹猴6只，年龄2~3岁，体重3.8~5.7 kg。恒温恒湿(28±1℃，50%~60%)条件下，恒温恒湿条件下，恒温恒温条件下，恒温恒湿条件下，恒温恒温，恒温恒温，恒温恒湿条件下，恒温恒湿条件下，恒温恒温，恒温恒湿条件下，恒温恒温条件下，恒温恒湿条件下(28±1℃，50%~60%)，恒温恒温条件下，恒温恒湿条件下，恒温条件下，恒温恒湿条件下，恒温，恒温恒湿条件下，恒温恒湿在全身麻醉下每周进行一次口腔卫生检查，以尽可能保持牙周组织的健康状态。分别在2%利多卡因、肾上腺素局部麻醉下拔除左右下颌第二前磨牙和第一磨牙。3个月后，将垂直松开的全厚粘骨膜瓣隆起，造成不同大小的人工牙周围骨缺损，对照组在根面刮除皮瓣闭合。Boneject组植入人工骨材料(日本Koken株式会社Boneject^[Enlosed R])，关闭皮瓣。膜组使用可吸收膜(Resolut^[Enloded R]，日本Gore-Tex，日本)。术后3个月处死动物，制作组织切片。结果：各组骨组织均充填2x1x1骨缺损区。对照组2x2x2或2x2x4骨缺损均无骨愈合。这些结果提示，对于较小的骨缺损，但大于8 mm^3的骨缺损，一些再生疗法对牙周组织的再生是有帮助的。

The Purpose of this study was to clarify the healing process and establish the standard of the effect of periodontal tissue re-generation ability. The experimental animals for make 3-wall bone defects were six male crab-eating monkeys (Macaca fascicularis), each monkeys were approximately 2 to 3 years of age and weighting 3.8 to 5.7 kg. The monkeys were kept in separate metal cages under constant temperature and humidistat conditions (28 ± 1℃, 50% to 60%) with sufficient solid monkey dietand tap of water. The oral hygiene procedures were performed once a week under general anesthesia to maintain the periodontal tissues in as healthy condition as possible. The test sites were the second premolar and first molar teeth in the right and left mandible.The second premolar and the first molar in the right and left mandible were extracted under local anesthesia with 2% lidocine, including epinephrine for hemostasis. Three months after, full-thickness mucoperiosteai flap with vertical releaseing incisions were raised, and made various size of artificial bone defect surrounding teeth.Control group were scaled the surface of the root and close the flap. Boneject group were implant artificial bone material (Boneject^【encircled R】, Koken Co.Ltd., Japan) and close the flap. Membrane group were used resorbable membrane (Resolut^【encircled R】, Japan Gore-tex, Japan). Three month after surgery, animals were sacrificed and made tissue section. In the results, 2x1x1 bone defect were fully filled by bone tissue in all groups. In contrast, 2x2x2 or 2x2x4 bone defects, they were no bony healing in the control group. In these results suggest that, they are no effect on the small bony defect but more than 8mm^3 of bony defect, some regenerative therapy will be usefull to regenerate periodontal tissue.