Preparation of soft and hard biodegradable materials for tissue engineering

组织工程软硬生物降解材料的制备

基本信息

批准号：
15300167
负责人：
AOYAGI Takao
金额：
$ 9.79万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

1.Soft MaterialsWe designed the hydrogel which has biodegradability and a temperature response for the three-dimensional matrix in tissue engineering. The materials was derived from two kinds of prepolymer, that is, IPAAm copolymer with activated carboxyl groups and one with poly(Ala-co-Gly) grafted chains, respectively. Only mixing of each aqueous prepolymer instanlt formed hydrogel and resulting one showed a clear volume phase transition temperature around 37℃. Moreover, we confirmed the enzymatic degradation below and above the transition temperature. In the well-swelled condition, the hydrogel was degraded gradually. This result suggested the hydrogel would be used for three-dimensional cell culture system.To obtain the functional monomer with isopropylacrylamide backbone, we designed and synthesized new type of monomers. These are 2-hydroxyisopropylacrylamide, N,N-dimthylaminoisopropylacrylamide. The copolymer comprising IPAAm and these monomers would be effective to modulate the … More transition temperature or to improve the cell ineraction with the synthesized matrix.2.Hard MaterialsTo obtain the hard biodegradable materials with easiness to design the desirable shape, we prepared the light-cross-linkable poly(ε-caprolactone-co-lactide). Actually, thermal propertied such as the melting points (softening points) closely depended on the composition of the caprolactone and the lactide in the cross-linked materials. We checked the cell adhesion on the prepared membrane-shaped materials using the model cell, conventionally cultured HeLa cell. Interestingly, the cell adhesion and growth depended on the composition of the caprolactone and the lactide. The rate of the growth is comparable to that of the tissue culture polystyrene (TCPS) or sometimes better. We also investigated the protein adsorption on the cross-linked materials using model protein, albumin and bovine serum. From the results, More protein adhered on the the materials that showed more cell adhesion and growth.From these projects, we succeeded the two types of the polymeric materials, such as enzymatically degradable hydrogels and cross-linked poly(ε-caprolactone-co-lactide) and these are useful for the tissue engineering. Less

1.Soft材料设计的水凝胶具有生物降解性和组织工程中三维基质的温度响应。这些材料源自两种前聚合物，即ipaam共聚物与活化的羧基和一种分别带有聚（Ala-co-gly）接枝链的共聚物。仅将每种水性聚合物插入式盐凝胶形成水凝胶并结果的混合显示明显的体积相变温度左右37℃。此外，我们确认了下方和高于过渡温度的酶促降解。在良好的条件下，水凝胶逐渐降解。该结果表明，水凝胶将用于三维细胞培养系统。要获得具有异丙基酰胺基骨架的功能单体，我们设计并合成了新型的单体类型。这些是2-羟羟丙烯酰胺，N，N-二硫代氨基二异丙丙烯酰胺。兼容的共聚物IPAAM和这些单体将有效调节……更高的过渡温度或通过合成矩阵改善细胞非相对性。2.hardMaterate之类的材料之类的可轻松设计可观的材料，以设计理想的形状，我们准备了可轻度的折叠（ε-caprolactone-co-co-copertional-crossied offertrential offertrential the Protertial offertrential intermential intermentical offertional cottrantimentimallentimentimallentimentimentimallentimentials）。取决于交联材料中丙二酮和乳酸的组成。我们使用模型细胞（常规培养的HeLa细胞）检查了准备好的膜形材料的细胞粘附。有趣的是，细胞的粘附和生长取决于丙二酮和乳酸的组成。生长的速率与组织培养的聚苯乙烯（TCP）或有时更好的速率相当。我们还使用模型蛋白质，白蛋白和牛血清研究了在交联材料上的蛋白质吸附。从结果来看，更多的蛋白质粘附在显示出更多细胞粘附和生长的材料上。从这些项目中，我们成功地取代了聚合物材料的两种类型，例如酶上可降解的水凝胶和交联的聚（ε-磷酸酯-co-lactide），并且这些对组织工程有用。较少的