Ultrasonic Nano-imaging of Vascular Endothelial Cell

血管内皮细胞的超声纳米成像

• 批准号: 15300178
• 负责人: SAIJO Yoshifumi
• 金额: $ 8.06万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15300178/
• 关键词: acoustic microscopy; sound speed; attenuation; phase; ultrasonic tissue characterization; biomechanics; cell; 血管内皮細胞

For precise imaging of cells, pre-treatments such as antibody sensing are needed in electron scanning microscopy and laser scanning microscopy with fluorescence technique. Non-contact nano-imaging of cells without pre-treatment is anticipated for repeated measurements under various conditions. The objective of the present research is to develop a nano-imaging system that can measure sound speed, attenuation and acoustic impedance of cells with high frequency ultrasound. Further, we compare the distribution of acoustic properties within a cell and cytoskeleton such as actin filaments or tubulin in order to prove the usefulness of the system in the measurement of cellular biomechanics.An electric pulse with the pulse width of 2 ns was generated by high speed mechanical switching was directly input into the ultrasonic transducer. The pulse response was analyzed by a digital oscilloscope. Two servo linear motors controlled by a microcomputer board were used for the scanning in both x- and y-axis of the imaging system. The total system was controlled by a workstation and 300x300 points data acquisition was done within two minutes. The interference between reflections from the surface of the specimen and the interface between the specimen and a glass slide was analyzed in frequency domain. The algorithm for calculation of the thickness and the sound speed of the specimen was established and finally two-dimensional mapping of quantitative sound speed distribution was displayed.Cooling effect of pulmonary vascular endothelial cells was investigated with actin filaments staining, electric impedance measurement and nano-imaging system. The results indicated the usefulness of the system for investigation of cellular biomechanics.

在电子扫描显微镜和激光扫描显微镜中，为了对细胞进行精确成像，需要进行抗体传感等预处理。预期在各种条件下进行重复测量时，无需预处理即可对细胞进行非接触式纳米成像。本研究的目的是开发一种纳米成像系统，可以测量声速，衰减和声阻抗的细胞与高频超声。此外，我们还比较了细胞和细胞骨架（如肌动蛋白丝或微管蛋白）内声学特性的分布，以证明该系统在细胞生物力学测量中的实用性。通过高速机械开关产生脉宽为2 ns的电脉冲，直接输入超声换能器。通过数字示波器分析脉冲响应。该成像系统采用两台直线伺服电机，分别在X轴和Y轴上进行扫描。整个系统由工作站控制，在两分钟内完成300 × 300点数据采集。在频域中分析了来自样品表面的反射与样品与载玻片之间的界面之间的干涉。建立了计算试件厚度和声速的算法，并最终显示了二维声速定量分布图，采用肌动蛋白染色、电阻抗测量和纳米成像系统研究了肺血管内皮细胞的降温效应。结果表明，该系统的细胞生物力学研究的有用性。

项目成果

Influence of tissue preparation on the acoustic properties of tissue sections at high frequencies.

组织制备对高频组织切片声学特性的影响。

• 发表时间: 2003
• 期刊: Ultrasound Med Biol 29・9
• 作者: Saijo Y et al.;Saijo Y et al.;Saijo Y et al.
• 通讯作者: Saijo Y et al.

Tissue velocity imaging of coronary artery by rotating-type intravascular ultrasound.

• DOI: 10.1016/j.ultras.2003.11.022
• 发表时间: 2004-04
• 期刊: Ultrasonics
• 影响因子: 4.2
• 作者: Y. Saijo;A. Tanaka;N. Owada;Y. Akino;S. Nitta

Fundamental study of ultrasonic-measurement-integrated simulation of real blood flow in the aorta

• DOI: 10.1007/s10439-005-2495-2
• 发表时间: 2005-04-01
• 期刊: ANNALS OF BIOMEDICAL ENGINEERING
• 影响因子: 3.8
• 作者: Funamoto, K;Hayase, T;Yambe, T
• 通讯作者: Yambe, T

Vascular Ultrasound

• DOI: 10.1016/j.emc.2024.05.011
• 发表时间: 2024-11-01
• 期刊: EMERGENCY MEDICINE CLINICS OF NORTH AMERICA
• 影响因子: 1.2
• 作者: Frederick，Meaghan K.;Stolz，Lori A.;Duran-Gehring，Petra E.
• 通讯作者: Duran-Gehring，Petra E.

Saijo Y, Kobayashi K, Arai H, et al.: "Pocket-size echo connectable to a personal computer"Ultrasound Med Biol. 29・5s. S54 (2003)

Saijo Y、Kobayashi K、Arai H 等人：“可连接到个人计算机的袖珍型回声”Ultrasound Med Biol. 29・5s。