The systematic approach for the understanding of dynamic aspects of chromosome VI of S.cerevisiae

理解酿酒酵母 VI 号染色体动态方面的系统方法

基本信息

批准号：
15310136
负责人：
SHIRAHIGE Katsuhiko
金额：
$ 10.05万
依托单位：
Tokyo Institute of Technology (2004-2005)The Institute of Physical and Chemical Research (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

The goal of this proposal is to build up the system that enables us to study the dynamic aspects of chromosome behavior through the analyses of protein binding profile data (ChIP-chip data) of various DNA binding proteins on S.cerevisiae chromosome VI (280kb).We have developed the software for calculation and visual presentation of the protein binding profile on chromosome VI. All of these software are available through our web site (http://shirahigelab.bio.titech.ac.jp). Now we are trying to expand the system so that analyses at whole genome level is possible for S.cerevisiae, S.pombe, and H.sapiens. Our analytical method is 100% compatible with MIAME checklist and other research groups utilized our system for the publication of their own data. All these suggest that the system we have built is now going to be a standard of ChIP-chip technique world wide (Methods in Enzymology, 2006). Proto-type system for the comparison and classification of protein binding profile data is also launched, and we have made the new achievements as follows using this new system. 1) Discovery of regulator of cohesin complex (nature, 2006), 2) Identification of Scc2, a cohesin loader, as a repair protein, 3) Dynamics of cohesin during S-phase (coupling of cohesin binding to chromosome with chromosomal DNA replication), 4) Identification of specific binding profile of SMC5/6 (Molecular Cell, 2006), and so on. All of these new findings identified cohesin as a key molecule that links various chromosome functions.

该提案的目的是建立一个系统，使我们能够通过分析蛋白质结合谱数据（CHIP-CHIP数据）来研究染色体行为的动态方面，该系统对酿酒酵母VI的各种DNA结合蛋白（280KB）（280KB）。我们已经开发了用于蛋白质结合型蛋白质结合体的软件。所有这些软件均可通过我们的网站（http://shirahigelab.bio.titech.ac.jp）获得。现在，我们正在尝试扩展系统，以便S.cerevisiae，S.Pombe和H.Sapiens可以在整个基因组水平上进行分析。我们的分析方法与迈阿密清单100％兼容，其他研究小组则利用我们的系统发布自己的数据。所有这些都表明，我们建立的系统现在将成为全球芯片芯片技术的标准（酶学方法，2006年）。还启动了用于比较和分类的原型系统，并启动了蛋白质结合曲线数据的分类，我们已经使用此新系统做出了新的成就。 1) Discovery of regulator of cohesin complex (nature, 2006), 2) Identification of Scc2, a cohesin loader, as a repair protein, 3) Dynamics of cohesin during S-phase (coupling of cohesin binding to chromosome with chromosomal DNA replication), 4) Identification of specific binding profile of SMC5/6 (Molecular Cell, 2006), and so on.所有这些新发现都将粘着蛋白确定为连接各种染色体功能的关键分子。